Epithelial G protein-coupled receptor kinases regulate the initial inflammatory response during mycobacterial infection.
Håkansson, Gisela LU ; Lutay, Nataliya LU ; Andersson, Märta LU ; Hallgren, Oskar LU ; Westergren-Thorsson, Gunilla LU
; Svensson, Majlis
LU
and Godaly, Gabriela
LU
(2013)
In Immunobiology
218(7).
p.984-994
- Abstract
- The interaction between mycobacteria and epithelium is unexplored, but may determine the outcome of the infection. We have analyzed the role of two G protein-coupled receptors, CXCR1 and CXCR2 that are important regulators of many pulmonary diseases. We found that mycobacteria significantly increased the expression of both CXCR1 and CXCR2 on alveolar epithelial cells and both receptors were found to be important for neutrophil diapedesis across primary endothelial cells towards infected mucosa. Mycobacteria, lipoarabinomannan or 19-kDa glycolipoprotein up-regulated the inhibitory G protein-coupled receptor kinase (GRK)2, while GRK3 was less affected. Mycobacteria-induced GRK2 up-regulation decreased chemokine transcription and secretion... (More)
- The interaction between mycobacteria and epithelium is unexplored, but may determine the outcome of the infection. We have analyzed the role of two G protein-coupled receptors, CXCR1 and CXCR2 that are important regulators of many pulmonary diseases. We found that mycobacteria significantly increased the expression of both CXCR1 and CXCR2 on alveolar epithelial cells and both receptors were found to be important for neutrophil diapedesis across primary endothelial cells towards infected mucosa. Mycobacteria, lipoarabinomannan or 19-kDa glycolipoprotein up-regulated the inhibitory G protein-coupled receptor kinase (GRK)2, while GRK3 was less affected. Mycobacteria-induced GRK2 up-regulation decreased chemokine transcription and secretion thereby affecting the neutrophil recruitment to infected mucosa. These events were completely abolished by blocking these receptors prior to infection as the blocking increased epithelial immune responses. We have identified novel interactions occurring in the initial phase of mycobacterial infections by which mycobacterial manipulate epithelial inflammatory responses. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3438783
- author
- Håkansson, Gisela
LU
; Lutay, Nataliya
LU
; Andersson, Märta
LU
; Hallgren, Oskar
LU
; Westergren-Thorsson, Gunilla
LU
; Svensson, Majlis
LU
and Godaly, Gabriela
LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Immunobiology
- volume
- 218
- issue
- 7
- pages
- 984 - 994
- publisher
- Elsevier
- external identifiers
-
- wos:000320485300007
- pmid:23312955
- scopus:84877141728
- pmid:23312955
- ISSN
- 1878-3279
- DOI
- 10.1016/j.imbio.2012.11.001
- language
- English
- LU publication?
- yes
- id
- 29c9581f-d903-4c78-a9fb-342992c1763f (old id 3438783)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/23312955?dopt=Abstract
- date added to LUP
- 2016-04-01 10:35:25
- date last changed
- 2024-04-07 13:35:13
@article{29c9581f-d903-4c78-a9fb-342992c1763f,
abstract = {{The interaction between mycobacteria and epithelium is unexplored, but may determine the outcome of the infection. We have analyzed the role of two G protein-coupled receptors, CXCR1 and CXCR2 that are important regulators of many pulmonary diseases. We found that mycobacteria significantly increased the expression of both CXCR1 and CXCR2 on alveolar epithelial cells and both receptors were found to be important for neutrophil diapedesis across primary endothelial cells towards infected mucosa. Mycobacteria, lipoarabinomannan or 19-kDa glycolipoprotein up-regulated the inhibitory G protein-coupled receptor kinase (GRK)2, while GRK3 was less affected. Mycobacteria-induced GRK2 up-regulation decreased chemokine transcription and secretion thereby affecting the neutrophil recruitment to infected mucosa. These events were completely abolished by blocking these receptors prior to infection as the blocking increased epithelial immune responses. We have identified novel interactions occurring in the initial phase of mycobacterial infections by which mycobacterial manipulate epithelial inflammatory responses.}},
author = {{Håkansson, Gisela and Lutay, Nataliya and Andersson, Märta and Hallgren, Oskar and Westergren-Thorsson, Gunilla and Svensson, Majlis and Godaly, Gabriela}},
issn = {{1878-3279}},
language = {{eng}},
number = {{7}},
pages = {{984--994}},
publisher = {{Elsevier}},
series = {{Immunobiology}},
title = {{Epithelial G protein-coupled receptor kinases regulate the initial inflammatory response during mycobacterial infection.}},
url = {{http://dx.doi.org/10.1016/j.imbio.2012.11.001}},
doi = {{10.1016/j.imbio.2012.11.001}},
volume = {{218}},
year = {{2013}},
}