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Increased C1q, C4 and C3 deposition on platelets in patients with systemic lupus erythematosus - a possible link to venous thrombosis?

Lood, Christian LU ; Eriksson, Sam LU ; Gullstrand, Birgitta LU ; Jönsen, Andreas LU ; Sturfelt, Gunnar LU ; Truedsson, Lennart LU and Bengtsson, Anders LU (2012) In Lupus 21(13). p.1423-1432
Abstract
Objective:

Patients with systemic lupus erythematosus (SLE) have an increased risk of developing vascular diseases (VD) such as myocardial infarction, stroke and venous thrombosis, which can only partly be explained by traditional risk factors. The role of platelets in this process has not been extensively studied. Platelet activation supports complement binding to the platelet surface, and increased C4d has been seen on platelets in SLE patients as well as in non-rheumatic patients with stroke. In this study we investigated in vivo platelet deposition of the classical complement pathway components C1q, C4d and C3d in relation to VD in SLE patients. Furthermore, the ability of serum to support in vitro complement deposition on... (More)
Objective:

Patients with systemic lupus erythematosus (SLE) have an increased risk of developing vascular diseases (VD) such as myocardial infarction, stroke and venous thrombosis, which can only partly be explained by traditional risk factors. The role of platelets in this process has not been extensively studied. Platelet activation supports complement binding to the platelet surface, and increased C4d has been seen on platelets in SLE patients as well as in non-rheumatic patients with stroke. In this study we investigated in vivo platelet deposition of the classical complement pathway components C1q, C4d and C3d in relation to VD in SLE patients. Furthermore, the ability of serum to support in vitro complement deposition on fixed heterologous platelets was analyzed.



Methods:

Blood from 69 SLE patients and age- and sex-matched healthy individuals was collected in sodium-citrate tubes and platelets isolated by centrifugation. Complement deposition on platelets was detected by flow cytometry.



Results:

We could demonstrate that SLE patients had increased C1q, C3d and C4d deposition on platelets as compared to healthy controls (p < 0.0001). SLE patients with a history of venous thrombosis had increased complement deposition on platelets as compared to SLE patients without this manifestation (p < 0.05). In vitro studies demonstrated that serum from patients with lupus anticoagulant, venous thrombosis or antiphospholipid antibody syndrome supported increased platelet C4d deposition in vitro as compared to SLE patients without these manifestations (p < 0.05). Our data support the hypothesis that platelet activation and the subsequent complement deposition on platelets are central in the development of venous thrombosis in SLE.



Conclusions:

Altogether we suggest that complement deposition on platelets could reflect important pathogenetic events related to the development of venous thrombosis in SLE and might be used as a marker for venous thrombosis in SLE. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Lupus
volume
21
issue
13
pages
1423 - 1432
publisher
SAGE Publications Ltd
external identifiers
  • wos:000310160700007
  • pmid:22878256
  • scopus:84867809391
ISSN
0961-2033
DOI
10.1177/0961203312457210
language
English
LU publication?
yes
id
5e4bb54b-b47d-4e06-93f9-adf606f71014 (old id 3047672)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22878256?dopt=Abstract
date added to LUP
2012-09-04 21:58:51
date last changed
2017-11-12 03:14:20
@article{5e4bb54b-b47d-4e06-93f9-adf606f71014,
  abstract     = {Objective: <br/><br>
Patients with systemic lupus erythematosus (SLE) have an increased risk of developing vascular diseases (VD) such as myocardial infarction, stroke and venous thrombosis, which can only partly be explained by traditional risk factors. The role of platelets in this process has not been extensively studied. Platelet activation supports complement binding to the platelet surface, and increased C4d has been seen on platelets in SLE patients as well as in non-rheumatic patients with stroke. In this study we investigated in vivo platelet deposition of the classical complement pathway components C1q, C4d and C3d in relation to VD in SLE patients. Furthermore, the ability of serum to support in vitro complement deposition on fixed heterologous platelets was analyzed. <br/><br>
<br/><br>
Methods: <br/><br>
Blood from 69 SLE patients and age- and sex-matched healthy individuals was collected in sodium-citrate tubes and platelets isolated by centrifugation. Complement deposition on platelets was detected by flow cytometry. <br/><br>
<br/><br>
Results: <br/><br>
We could demonstrate that SLE patients had increased C1q, C3d and C4d deposition on platelets as compared to healthy controls (p &lt; 0.0001). SLE patients with a history of venous thrombosis had increased complement deposition on platelets as compared to SLE patients without this manifestation (p &lt; 0.05). In vitro studies demonstrated that serum from patients with lupus anticoagulant, venous thrombosis or antiphospholipid antibody syndrome supported increased platelet C4d deposition in vitro as compared to SLE patients without these manifestations (p &lt; 0.05). Our data support the hypothesis that platelet activation and the subsequent complement deposition on platelets are central in the development of venous thrombosis in SLE. <br/><br>
<br/><br>
Conclusions: <br/><br>
Altogether we suggest that complement deposition on platelets could reflect important pathogenetic events related to the development of venous thrombosis in SLE and might be used as a marker for venous thrombosis in SLE.},
  author       = {Lood, Christian and Eriksson, Sam and Gullstrand, Birgitta and Jönsen, Andreas and Sturfelt, Gunnar and Truedsson, Lennart and Bengtsson, Anders},
  issn         = {0961-2033},
  language     = {eng},
  number       = {13},
  pages        = {1423--1432},
  publisher    = {SAGE Publications Ltd},
  series       = {Lupus},
  title        = {Increased C1q, C4 and C3 deposition on platelets in patients with systemic lupus erythematosus - a possible link to venous thrombosis?},
  url          = {http://dx.doi.org/10.1177/0961203312457210},
  volume       = {21},
  year         = {2012},
}