Retained heterodisomy is associated with high gene expression in hyperhaploid inflammatory leiomyosarcoma.
(2012) In Neoplasia 14(9). p.807-812- Abstract
- Inflammatory leiomyosarcoma (ILMS) is a soft tissue tumor that morphologically resembles conventional leiomyosarcoma (LMS) admixed with a prominent inflammatory infiltrate. Genetic data on ILMS are still limited but have suggested that this entity is characterized by hyperhaploidy (24-34 chromosomes). This low chromosome number is otherwise uncommon in neoplasia and has been found only in 0.2% to 0.3% of cytogenetically investigated tumors. Here, three ILMS were investigated using cytogenetic, single-nucleotide polymorphism (SNP) array, and global gene expression analyses. All cases displayed a hyperhaploid origin. Combined with previously reported cases, hyperhaploidy has been found in six of seven cytogenetically investigated ILMS. The... (More)
- Inflammatory leiomyosarcoma (ILMS) is a soft tissue tumor that morphologically resembles conventional leiomyosarcoma (LMS) admixed with a prominent inflammatory infiltrate. Genetic data on ILMS are still limited but have suggested that this entity is characterized by hyperhaploidy (24-34 chromosomes). This low chromosome number is otherwise uncommon in neoplasia and has been found only in 0.2% to 0.3% of cytogenetically investigated tumors. Here, three ILMS were investigated using cytogenetic, single-nucleotide polymorphism (SNP) array, and global gene expression analyses. All cases displayed a hyperhaploid origin. Combined with previously reported cases, hyperhaploidy has been found in six of seven cytogenetically investigated ILMS. The copy number distribution of individual chromosomes is clearly nonrandom; the hyperhaploid clones of all six cases displayed disomy for chromosomes 5 and 20, and two copies of chromosomes 18, 21, and 22 were also common. All chromosomes identified as disomic showed a biparental origin by SNP array analysis; whether this is of pathogenetic importance is not known. Compared with conventional LMS, ILMS had a distinct gene expression signature. Furthermore, the number of chromosome copies correlated well with gene expression levels; disomic chromosomes showed higher gene expression levels than monosomic chromosomes, a finding that has not previously been reported for hyperhaploid tumors. Taken together, our findings suggest that disomy for some chromosomes, notably 5 and 20, as well as distorted gene expression achieved through massive loss of other chromosomes are essential features of ILMS. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3123527
- author
- Hansén Nord, Karolin LU ; Paulsson, Kajsa LU ; Veerla, Srinivas LU ; Wejde, Johan ; Brosjö, Otte ; Mandahl, Nils LU and Mertens, Fredrik LU
- organization
-
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
- Division of Clinical Genetics
- Breast/lung cancer (research group)
- Division of Translational Cancer Research
- Breast and Ovarian Cancer Genomics (research group)
- Research Group Lung Cancer (research group)
- Breast/lungcancer
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Neoplasia
- volume
- 14
- issue
- 9
- pages
- 807 - 812
- publisher
- Neoplasia Press
- external identifiers
-
- wos:000313345200004
- pmid:23019412
- scopus:84866550537
- ISSN
- 1522-8002
- language
- English
- LU publication?
- yes
- id
- 87892d99-132d-48eb-8a96-d388da4d309c (old id 3123527)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/23019412?dopt=Abstract
- date added to LUP
- 2016-04-04 08:24:33
- date last changed
- 2024-02-11 00:10:29
@article{87892d99-132d-48eb-8a96-d388da4d309c, abstract = {{Inflammatory leiomyosarcoma (ILMS) is a soft tissue tumor that morphologically resembles conventional leiomyosarcoma (LMS) admixed with a prominent inflammatory infiltrate. Genetic data on ILMS are still limited but have suggested that this entity is characterized by hyperhaploidy (24-34 chromosomes). This low chromosome number is otherwise uncommon in neoplasia and has been found only in 0.2% to 0.3% of cytogenetically investigated tumors. Here, three ILMS were investigated using cytogenetic, single-nucleotide polymorphism (SNP) array, and global gene expression analyses. All cases displayed a hyperhaploid origin. Combined with previously reported cases, hyperhaploidy has been found in six of seven cytogenetically investigated ILMS. The copy number distribution of individual chromosomes is clearly nonrandom; the hyperhaploid clones of all six cases displayed disomy for chromosomes 5 and 20, and two copies of chromosomes 18, 21, and 22 were also common. All chromosomes identified as disomic showed a biparental origin by SNP array analysis; whether this is of pathogenetic importance is not known. Compared with conventional LMS, ILMS had a distinct gene expression signature. Furthermore, the number of chromosome copies correlated well with gene expression levels; disomic chromosomes showed higher gene expression levels than monosomic chromosomes, a finding that has not previously been reported for hyperhaploid tumors. Taken together, our findings suggest that disomy for some chromosomes, notably 5 and 20, as well as distorted gene expression achieved through massive loss of other chromosomes are essential features of ILMS.}}, author = {{Hansén Nord, Karolin and Paulsson, Kajsa and Veerla, Srinivas and Wejde, Johan and Brosjö, Otte and Mandahl, Nils and Mertens, Fredrik}}, issn = {{1522-8002}}, language = {{eng}}, number = {{9}}, pages = {{807--812}}, publisher = {{Neoplasia Press}}, series = {{Neoplasia}}, title = {{Retained heterodisomy is associated with high gene expression in hyperhaploid inflammatory leiomyosarcoma.}}, url = {{http://www.ncbi.nlm.nih.gov/pubmed/23019412?dopt=Abstract}}, volume = {{14}}, year = {{2012}}, }