Inflammatory reaction in the retina after focal non-convulsive status epilepticus in mice investigated with high resolution magnetic resonance and diffusion tensor imaging
(2021) In Epilepsy Research 176.- Abstract
- Pathophysiological consequences of focal non-convulsive status epilepticus (fNCSE) have been difficult to demonstrate in humans. In rats fNCSE pathology has been identified in the eyes. Here we evaluated the use of high-resolution 7 T structural T1-weighted magnetic resonance imaging (MRI) and 9.4 T diffusion tensor imaging (DTI) for detecting hippocampal fNCSE-induced retinal pathology ex vivo in mice. Seven weeks post-fNCSE, increased number of Iba1+ microglia were evident in the retina ipsilateral to the hemisphere with fNCSE, and morphologically more activated microglia were found in both ipsi- and contralateral retina compared to non-stimulated control mice. T1-weighted intensity measurements of the contralateral retina showed a minor... (More)
- Pathophysiological consequences of focal non-convulsive status epilepticus (fNCSE) have been difficult to demonstrate in humans. In rats fNCSE pathology has been identified in the eyes. Here we evaluated the use of high-resolution 7 T structural T1-weighted magnetic resonance imaging (MRI) and 9.4 T diffusion tensor imaging (DTI) for detecting hippocampal fNCSE-induced retinal pathology ex vivo in mice. Seven weeks post-fNCSE, increased number of Iba1+ microglia were evident in the retina ipsilateral to the hemisphere with fNCSE, and morphologically more activated microglia were found in both ipsi- and contralateral retina compared to non-stimulated control mice. T1-weighted intensity measurements of the contralateral retina showed a minor increase within the outer nuclear and plexiform layers of the lateral retina. T1-weighted measurements were not performed in the ipsilateral retina due to technical difficulties. DTI fractional anisotropy(FA) values were discretely altered in the lateral part of the ipsilateral retina and unaltered in the contralateral retina. No changes were observed in the distal part of the optic nerve. The sensitivity of both imaging techniques for identifying larger retinal alteration was confirmed ex vivo in retinitis pigmentosa mice where a substantial neurodegeneration of the outer retinal layers is evident. With MR imaging a 50 % decrease in DTI FA values and significantly thinner retina in T1-weighted images were detected. We conclude that retinal pathology after fNCSE in mice is subtle and present bilaterally. High-resolution T1-weighted MRI and DTI independently did not detect the entire pathological retinal changes after fNCSE, but the combination of the two techniques indicated minor patchy structural changes. (Less)
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https://lup.lub.lu.se/record/320f31d5-1898-4609-89a5-e82228179d29
- author
- organization
-
- Clinical Neurophysiology
- Inflammation and Stemcell Therapy Group (research group)
- Neurogenesis and cell therapy (research group)
- Clinical Epilepsy Research (research group)
- Neurology, Lund
- WCMM-Wallenberg Centre for Molecular Medicine
- Nanostructures in neural systems (research group)
- Ophthalmology, Lund
- Epilepsy Center
- publishing date
- 2021-07-24
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- DTI, MRI, non-convulsive status epilepticus, Epilepsy, Retina, Inflammation
- in
- Epilepsy Research
- volume
- 176
- article number
- 106730
- publisher
- Elsevier
- external identifiers
-
- pmid:34364020
- scopus:85111808466
- ISSN
- 0920-1211
- DOI
- 10.1016/j.eplepsyres.2021.106730
- language
- English
- LU publication?
- yes
- id
- 320f31d5-1898-4609-89a5-e82228179d29
- date added to LUP
- 2021-09-07 11:48:26
- date last changed
- 2022-04-27 03:42:26
@article{320f31d5-1898-4609-89a5-e82228179d29,
abstract = {{Pathophysiological consequences of focal non-convulsive status epilepticus (fNCSE) have been difficult to demonstrate in humans. In rats fNCSE pathology has been identified in the eyes. Here we evaluated the use of high-resolution 7 T structural T1-weighted magnetic resonance imaging (MRI) and 9.4 T diffusion tensor imaging (DTI) for detecting hippocampal fNCSE-induced retinal pathology ex vivo in mice. Seven weeks post-fNCSE, increased number of Iba1+ microglia were evident in the retina ipsilateral to the hemisphere with fNCSE, and morphologically more activated microglia were found in both ipsi- and contralateral retina compared to non-stimulated control mice. T1-weighted intensity measurements of the contralateral retina showed a minor increase within the outer nuclear and plexiform layers of the lateral retina. T1-weighted measurements were not performed in the ipsilateral retina due to technical difficulties. DTI fractional anisotropy(FA) values were discretely altered in the lateral part of the ipsilateral retina and unaltered in the contralateral retina. No changes were observed in the distal part of the optic nerve. The sensitivity of both imaging techniques for identifying larger retinal alteration was confirmed ex vivo in retinitis pigmentosa mice where a substantial neurodegeneration of the outer retinal layers is evident. With MR imaging a 50 % decrease in DTI FA values and significantly thinner retina in T1-weighted images were detected. We conclude that retinal pathology after fNCSE in mice is subtle and present bilaterally. High-resolution T1-weighted MRI and DTI independently did not detect the entire pathological retinal changes after fNCSE, but the combination of the two techniques indicated minor patchy structural changes.}},
author = {{Ahl, Matilda and Lindbäck, Una and Chary, Karthic and Shibata, Keisuke and Chugh, Deepti and Linden Mickelsson, Pernilla and Kettunen, Mikko and COMPAGNO STRANDBERG, MARIA and Englund Johansson, Ulrica and Sierra, Alejandra and Ekdahl, Christine T}},
issn = {{0920-1211}},
keywords = {{DTI; MRI; non-convulsive status epilepticus; Epilepsy; Retina; Inflammation}},
language = {{eng}},
month = {{07}},
publisher = {{Elsevier}},
series = {{Epilepsy Research}},
title = {{Inflammatory reaction in the retina after focal non-convulsive status epilepticus in mice investigated with high resolution magnetic resonance and diffusion tensor imaging}},
url = {{https://lup.lub.lu.se/search/files/102107750/Ahl_M_et_al_2021.pdf}},
doi = {{10.1016/j.eplepsyres.2021.106730}},
volume = {{176}},
year = {{2021}},
}