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Development of a Hybrid Nanoprobe for Triple-Modality MR/SPECT/Optical Fluorescence Imaging

Madru, Renata LU ; Svenmarker, Pontus LU ; Ingvar, Christian LU ; Ståhlberg, Freddy LU ; Andersson-Engels, Stefan LU ; Knutsson, Linda LU and Strand, Sven-Erik LU (2014) In Diagnostics 4(1). p.13-26
Abstract (Swedish)
Hybrid clinical imaging is an emerging technology, which improves disease diagnosis by combining already existing technologies. With the combination of high-resolution morphological imaging, i.e., MRI/CT, and high-sensitive molecular detection offered by SPECT/PET/Optical, physicians can detect disease progression at an early stage and design patient-specific treatments. To fully exploit the possibilities of hybrid imaging a hybrid probe compatible with each imaging technology is required. Here, we present a hybrid nanoprobe for triple modality MR/SPECT/Fluorescence imaging. Our imaging agent is comprised of superparamagnetic iron oxide nanoparticles (SPIONs), labeled with 99mTc and an Alexa fluorophore (AF), together forming... (More)
Hybrid clinical imaging is an emerging technology, which improves disease diagnosis by combining already existing technologies. With the combination of high-resolution morphological imaging, i.e., MRI/CT, and high-sensitive molecular detection offered by SPECT/PET/Optical, physicians can detect disease progression at an early stage and design patient-specific treatments. To fully exploit the possibilities of hybrid imaging a hybrid probe compatible with each imaging technology is required. Here, we present a hybrid nanoprobe for triple modality MR/SPECT/Fluorescence imaging. Our imaging agent is comprised of superparamagnetic iron oxide nanoparticles (SPIONs), labeled with 99mTc and an Alexa fluorophore (AF), together forming 99mTc-AF-SPIONs. The agent was stable in human serum, and, after subcutaneous injection in the hind paw of Wistar rats, showed to be highly specific by accumulating in the sentinel lymph node. All three modalities clearly visualized the imaging agent. Our results show that a single imaging agent can be used for hybrid imaging. The use of a single hybrid contrast agent permits simultaneous hybrid imaging and, more conventionally, allow for single modality imaging at different time points. For example, a hybrid contrast agent enables pre-operative planning, intra-operative guidance, and post-operative evaluation with the same contrast agent.
Keywords: SPION, SPECT, optical, fluorescence imaging, magnetic resonance imaging, MR, SLN (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Diagnostics
volume
4
issue
1
pages
13 - 26
publisher
MDPI AG
ISSN
2075-4418
DOI
10.3390/diagnostics4010013
language
English
LU publication?
yes
id
3233a2c3-0ab8-437b-b7e8-73beea6a7241
date added to LUP
2017-03-09 11:39:59
date last changed
2017-03-10 14:50:17
@article{3233a2c3-0ab8-437b-b7e8-73beea6a7241,
  abstract     = {Hybrid clinical imaging is an emerging technology, which improves disease diagnosis by combining already existing technologies. With the combination of high-resolution morphological imaging, i.e., MRI/CT, and high-sensitive molecular detection offered by SPECT/PET/Optical, physicians can detect disease progression at an early stage and design patient-specific treatments. To fully exploit the possibilities of hybrid imaging a hybrid probe compatible with each imaging technology is required. Here, we present a hybrid nanoprobe for triple modality MR/SPECT/Fluorescence imaging. Our imaging agent is comprised of superparamagnetic iron oxide nanoparticles (SPIONs), labeled with 99mTc and an Alexa fluorophore (AF), together forming 99mTc-AF-SPIONs. The agent was stable in human serum, and, after subcutaneous injection in the hind paw of Wistar rats, showed to be highly specific by accumulating in the sentinel lymph node. All three modalities clearly visualized the imaging agent. Our results show that a single imaging agent can be used for hybrid imaging. The use of a single hybrid contrast agent permits simultaneous hybrid imaging and, more conventionally, allow for single modality imaging at different time points. For example, a hybrid contrast agent enables pre-operative planning, intra-operative guidance, and post-operative evaluation with the same contrast agent.<br/>Keywords: SPION, SPECT, optical, fluorescence imaging, magnetic resonance imaging, MR, SLN},
  author       = {Madru, Renata and Svenmarker, Pontus and Ingvar, Christian and Ståhlberg, Freddy and Andersson-Engels, Stefan and Knutsson, Linda and Strand, Sven-Erik},
  issn         = {2075-4418},
  language     = {eng},
  month        = {03},
  number       = {1},
  pages        = {13--26},
  publisher    = {MDPI AG},
  series       = {Diagnostics},
  title        = {Development of a Hybrid Nanoprobe for Triple-Modality MR/SPECT/Optical Fluorescence Imaging},
  url          = {http://dx.doi.org/10.3390/diagnostics4010013},
  volume       = {4},
  year         = {2014},
}