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Use of PLL-g-PEG in micro-fluidic devices for localizing selective and specific protein binding

Marie, Rodolphe ; Beech, Jason LU ; Voeroes, Janos ; Tegenfeldt, Jonas LU orcid and Höök, Fredrik (2006) In Langmuir 22(24). p.10103-10108
Abstract
By utilizing flow-controlled PLL-g-PEG and PLL-g-PEGbiotin modification of predefined regions of a poly-(dimethylsiloxane) (PDMS) micro-fluidic device, with an intentionally chosen large (similar to 1 cm(2)) internal surface area, we report rapid (10 min), highly localized (6 x 10(-6) cm(2)), and specific surface-based protein capture from a sample volume (100 mu L) containing a low amount of protein (160 attomol in pure buffer and 400 attomol in serum). The design criteria for this surface modification were achieved using QCM-D (quartz crystal microbalance with energy dissipation monitoring) of serum protein adsorption onto PLL-g-PEG-modified oxidized PDMS. Equally good, or almost as good, results were obtained for oxidized SU-8, Topas,... (More)
By utilizing flow-controlled PLL-g-PEG and PLL-g-PEGbiotin modification of predefined regions of a poly-(dimethylsiloxane) (PDMS) micro-fluidic device, with an intentionally chosen large (similar to 1 cm(2)) internal surface area, we report rapid (10 min), highly localized (6 x 10(-6) cm(2)), and specific surface-based protein capture from a sample volume (100 mu L) containing a low amount of protein (160 attomol in pure buffer and 400 attomol in serum). The design criteria for this surface modification were achieved using QCM-D (quartz crystal microbalance with energy dissipation monitoring) of serum protein adsorption onto PLL-g-PEG-modified oxidized PDMS. Equally good, or almost as good, results were obtained for oxidized SU-8, Topas, and poly(methyl metacrylate) (PMMA), demonstrating the generic potential of PLL-g-PEG for surface modification in various micro-fluidic applications. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
22
issue
24
pages
10103 - 10108
publisher
The American Chemical Society (ACS)
external identifiers
  • wos:000242022100044
  • scopus:33846121311
  • pmid:17107006
ISSN
0743-7463
DOI
10.1021/la060198m
language
English
LU publication?
yes
id
8c0875d3-e6a3-4816-8586-d59bf7dff685 (old id 376914)
date added to LUP
2016-04-01 11:37:28
date last changed
2021-09-15 05:25:36
@article{8c0875d3-e6a3-4816-8586-d59bf7dff685,
  abstract     = {By utilizing flow-controlled PLL-g-PEG and PLL-g-PEGbiotin modification of predefined regions of a poly-(dimethylsiloxane) (PDMS) micro-fluidic device, with an intentionally chosen large (similar to 1 cm(2)) internal surface area, we report rapid (10 min), highly localized (6 x 10(-6) cm(2)), and specific surface-based protein capture from a sample volume (100 mu L) containing a low amount of protein (160 attomol in pure buffer and 400 attomol in serum). The design criteria for this surface modification were achieved using QCM-D (quartz crystal microbalance with energy dissipation monitoring) of serum protein adsorption onto PLL-g-PEG-modified oxidized PDMS. Equally good, or almost as good, results were obtained for oxidized SU-8, Topas, and poly(methyl metacrylate) (PMMA), demonstrating the generic potential of PLL-g-PEG for surface modification in various micro-fluidic applications.},
  author       = {Marie, Rodolphe and Beech, Jason and Voeroes, Janos and Tegenfeldt, Jonas and Höök, Fredrik},
  issn         = {0743-7463},
  language     = {eng},
  number       = {24},
  pages        = {10103--10108},
  publisher    = {The American Chemical Society (ACS)},
  series       = {Langmuir},
  title        = {Use of PLL-g-PEG in micro-fluidic devices for localizing selective and specific protein binding},
  url          = {http://dx.doi.org/10.1021/la060198m},
  doi          = {10.1021/la060198m},
  volume       = {22},
  year         = {2006},
}