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Mechanical degradation and changes in conformation of hydrophobically modified starch

Nilsson, Lars LU ; Leeman, Mats LU ; Wahlund, Karl-Gustav LU and Bergenståhl, Björn LU (2006) In Biomacromolecules 7(9). p.2671-2679
Abstract
In this paper, we study the mechanical degradation and changes in conformation of a branched ultrahigh molar mass biomacromolecule, hydrophobically modified starch, as caused by high-pressure homogenization. The characterization was performed with asymmetrical flow field-flow fractionation (AsFlFFF) with multiangle light scattering (MALS) and refractive index detection. The starch which had been chemically modified with octenyl succinate anhydride (OSA) proved to be very large and polydisperse. Upon high-pressure homogenization, the molar mass and rms radius (r(rms)) decreased, and the extent of these changes was related to the turbulent flow conditions during homogenization. The treatment also induced an increase and scaling with size in... (More)
In this paper, we study the mechanical degradation and changes in conformation of a branched ultrahigh molar mass biomacromolecule, hydrophobically modified starch, as caused by high-pressure homogenization. The characterization was performed with asymmetrical flow field-flow fractionation (AsFlFFF) with multiangle light scattering (MALS) and refractive index detection. The starch which had been chemically modified with octenyl succinate anhydride (OSA) proved to be very large and polydisperse. Upon high-pressure homogenization, the molar mass and rms radius (r(rms)) decreased, and the extent of these changes was related to the turbulent flow conditions during homogenization. The treatment also induced an increase and scaling with size in the apparent density of the macromolecules. To further study the changes in conformation, it was necessary to calculate the hydrodynamic radii (r(h)). This can be determined numerically from the elution times in the analysis and the flow conditions in the AsFlFFF channel. The results showed that the treatment can cause a dramatic decrease in the quotient between r(rms) and r(h), suggesting major conformational changes. These results together could be interpreted as degradation and "crumpling" of the macromolecule, which would give a decrease in rrms and an increase in apparent density, together with a "fraying" of more outer parts of the macromolecule, which could give rise to the increase in r(h). (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biomacromolecules
volume
7
issue
9
pages
2671 - 2679
publisher
The American Chemical Society (ACS)
external identifiers
  • wos:000240403300028
  • scopus:33749543203
  • pmid:16961331
ISSN
1526-4602
DOI
10.1021/bm060367h
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Food Technology (011001017), Analytical Chemistry (S/LTH) (011001004)
id
0587f91b-a76c-4a8a-a383-fbc6758d0ed0 (old id 394278)
date added to LUP
2016-04-01 12:31:19
date last changed
2021-04-20 01:05:15
@article{0587f91b-a76c-4a8a-a383-fbc6758d0ed0,
  abstract     = {In this paper, we study the mechanical degradation and changes in conformation of a branched ultrahigh molar mass biomacromolecule, hydrophobically modified starch, as caused by high-pressure homogenization. The characterization was performed with asymmetrical flow field-flow fractionation (AsFlFFF) with multiangle light scattering (MALS) and refractive index detection. The starch which had been chemically modified with octenyl succinate anhydride (OSA) proved to be very large and polydisperse. Upon high-pressure homogenization, the molar mass and rms radius (r(rms)) decreased, and the extent of these changes was related to the turbulent flow conditions during homogenization. The treatment also induced an increase and scaling with size in the apparent density of the macromolecules. To further study the changes in conformation, it was necessary to calculate the hydrodynamic radii (r(h)). This can be determined numerically from the elution times in the analysis and the flow conditions in the AsFlFFF channel. The results showed that the treatment can cause a dramatic decrease in the quotient between r(rms) and r(h), suggesting major conformational changes. These results together could be interpreted as degradation and "crumpling" of the macromolecule, which would give a decrease in rrms and an increase in apparent density, together with a "fraying" of more outer parts of the macromolecule, which could give rise to the increase in r(h).},
  author       = {Nilsson, Lars and Leeman, Mats and Wahlund, Karl-Gustav and Bergenståhl, Björn},
  issn         = {1526-4602},
  language     = {eng},
  number       = {9},
  pages        = {2671--2679},
  publisher    = {The American Chemical Society (ACS)},
  series       = {Biomacromolecules},
  title        = {Mechanical degradation and changes in conformation of hydrophobically modified starch},
  url          = {http://dx.doi.org/10.1021/bm060367h},
  doi          = {10.1021/bm060367h},
  volume       = {7},
  year         = {2006},
}