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Yippee like 4 (Ypel4) is essential for normal mouse red blood cell membrane integrity

Mattebo, Alexander LU ; Sen, Taha LU ; Jassinskaja, Maria LU ; Pimková, Kristýna LU ; Prieto González-Albo, Isabel ; Alattar, Abdul Ghani LU orcid ; Ramakrishnan, Ramprasad LU ; Lang, Stefan LU orcid ; Järås, Marcus LU and Hansson, Jenny LU orcid , et al. (2021) In Scientific Reports 11(1).
Abstract

The YPEL family genes are highly conserved across a diverse range of eukaryotic organisms and thus potentially involved in essential cellular processes. Ypel4, one of five YPEL family gene orthologs in mouse and human, is highly and specifically expressed in late terminal erythroid differentiation (TED). In this study, we investigated the role of Ypel4 in murine erythropoiesis, providing for the first time an in-depth description of a Ypel4-null phenotype in vivo. We demonstrated that the Ypel4-null mice displayed a secondary polycythemia with macro- and reticulocytosis. While lack of Ypel4 did not affect steady-state TED in the bone marrow or spleen, the anemia-recovering capacity of Ypel4-null cells was diminished. Furthermore,... (More)

The YPEL family genes are highly conserved across a diverse range of eukaryotic organisms and thus potentially involved in essential cellular processes. Ypel4, one of five YPEL family gene orthologs in mouse and human, is highly and specifically expressed in late terminal erythroid differentiation (TED). In this study, we investigated the role of Ypel4 in murine erythropoiesis, providing for the first time an in-depth description of a Ypel4-null phenotype in vivo. We demonstrated that the Ypel4-null mice displayed a secondary polycythemia with macro- and reticulocytosis. While lack of Ypel4 did not affect steady-state TED in the bone marrow or spleen, the anemia-recovering capacity of Ypel4-null cells was diminished. Furthermore, Ypel4-null red blood cells (RBC) were cleared from the circulation at an increased rate, demonstrating an intrinsic defect of RBCs. Scanning electron micrographs revealed an ovalocytic morphology of Ypel4-null RBCs and functional testing confirmed reduced deformability. Even though Band 3 protein levels were shown to be reduced in Ypel4-null RBC membranes, we could not find support for a physical interaction between YPEL4 and the Band 3 protein. In conclusion, our findings provide crucial insights into the role of Ypel4 in preserving normal red cell membrane integrity.

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publishing date
type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
11
issue
1
article number
15898
publisher
Nature Publishing Group
external identifiers
  • pmid:34354145
  • scopus:85112003992
ISSN
2045-2322
DOI
10.1038/s41598-021-95291-1
language
English
LU publication?
yes
id
3a841e96-d9f7-4a45-b6a6-aef390a07474
date added to LUP
2021-09-03 11:36:07
date last changed
2024-06-01 14:52:33
@article{3a841e96-d9f7-4a45-b6a6-aef390a07474,
  abstract     = {{<p>The YPEL family genes are highly conserved across a diverse range of eukaryotic organisms and thus potentially involved in essential cellular processes. Ypel4, one of five YPEL family gene orthologs in mouse and human, is highly and specifically expressed in late terminal erythroid differentiation (TED). In this study, we investigated the role of Ypel4 in murine erythropoiesis, providing for the first time an in-depth description of a Ypel4-null phenotype in vivo. We demonstrated that the Ypel4-null mice displayed a secondary polycythemia with macro- and reticulocytosis. While lack of Ypel4 did not affect steady-state TED in the bone marrow or spleen, the anemia-recovering capacity of Ypel4-null cells was diminished. Furthermore, Ypel4-null red blood cells (RBC) were cleared from the circulation at an increased rate, demonstrating an intrinsic defect of RBCs. Scanning electron micrographs revealed an ovalocytic morphology of Ypel4-null RBCs and functional testing confirmed reduced deformability. Even though Band 3 protein levels were shown to be reduced in Ypel4-null RBC membranes, we could not find support for a physical interaction between YPEL4 and the Band 3 protein. In conclusion, our findings provide crucial insights into the role of Ypel4 in preserving normal red cell membrane integrity.</p>}},
  author       = {{Mattebo, Alexander and Sen, Taha and Jassinskaja, Maria and Pimková, Kristýna and Prieto González-Albo, Isabel and Alattar, Abdul Ghani and Ramakrishnan, Ramprasad and Lang, Stefan and Järås, Marcus and Hansson, Jenny and Soneji, Shamit and Singbrant, Sofie and van den Akker, Emile and Flygare, Johan}},
  issn         = {{2045-2322}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Scientific Reports}},
  title        = {{Yippee like 4 (Ypel4) is essential for normal mouse red blood cell membrane integrity}},
  url          = {{http://dx.doi.org/10.1038/s41598-021-95291-1}},
  doi          = {{10.1038/s41598-021-95291-1}},
  volume       = {{11}},
  year         = {{2021}},
}