DNA digestion and formation of DNA-network structures with Holliday junction-resolving enzyme Hjc_15-6 in conjunction with polymerase reactions
(2024) In Journal of Biotechnology- Abstract
- The recently identified novel Holliday junction-resolving enzyme, termed Hjc_15-6, activity investigation results imply DNA cleavage by Hjc_15-6 in a manner that potentially enhances the molecular self-assembly that may be exploited for creating DNA-networks and nanostructures. The study also demonstrates Pwo DNA polymerase acting in combination with Hjc_15-6 capability to produce large amounts of DNA that transforms into large DNA-network structures even without DNA template and primers. Furthermore, it is demonstrated that Hjc_15-6 prefers Holliday junction oligonucleotides as compared to Y-shaped oligonucleotides as well as efficiently cleaves typical branched products from isothermal DNA amplification of both linear and circular DNA... (More)
- The recently identified novel Holliday junction-resolving enzyme, termed Hjc_15-6, activity investigation results imply DNA cleavage by Hjc_15-6 in a manner that potentially enhances the molecular self-assembly that may be exploited for creating DNA-networks and nanostructures. The study also demonstrates Pwo DNA polymerase acting in combination with Hjc_15-6 capability to produce large amounts of DNA that transforms into large DNA-network structures even without DNA template and primers. Furthermore, it is demonstrated that Hjc_15-6 prefers Holliday junction oligonucleotides as compared to Y-shaped oligonucleotides as well as efficiently cleaves typical branched products from isothermal DNA amplification of both linear and circular DNA templates amplified by phi29-like DNA polymerase. The assembly of large DNA network structures was observed in real time, by transmission electron microscopy, on negative stained grids that were freshly prepared, and also on the same grids after incubation for 4 days under constant cooling. Hence, Hjc_15-6 is a promising molecular tool for efficient production of various DNA origamis that may be implemented for a wide range of applications such as within medical biomaterials, catalytic materials, molecular devices and biosensors. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3aff0f7f-e3ab-43f8-953d-2dca839e1fc7
- author
- Ahlqvist, Josefin LU ; Tymecka-Mulik, Joanna ; Burkiewicz, Katarzyna ; Jasilionis, Andrius LU ; Wallenberg, Reine LU ; Nordberg Karlsson, Eva LU and Dabrowski, Slawomir
- organization
- publishing date
- 2024-02-24
- type
- Contribution to journal
- publication status
- epub
- subject
- in
- Journal of Biotechnology
- publisher
- Elsevier
- external identifiers
-
- scopus:85187198518
- ISSN
- 1873-4863
- DOI
- 10.1016/j.jbiotec.2024.02.012
- language
- English
- LU publication?
- yes
- id
- 3aff0f7f-e3ab-43f8-953d-2dca839e1fc7
- date added to LUP
- 2024-02-25 18:35:47
- date last changed
- 2024-04-03 09:48:53
@article{3aff0f7f-e3ab-43f8-953d-2dca839e1fc7, abstract = {{The recently identified novel Holliday junction-resolving enzyme, termed Hjc_15-6, activity investigation results imply DNA cleavage by Hjc_15-6 in a manner that potentially enhances the molecular self-assembly that may be exploited for creating DNA-networks and nanostructures. The study also demonstrates Pwo DNA polymerase acting in combination with Hjc_15-6 capability to produce large amounts of DNA that transforms into large DNA-network structures even without DNA template and primers. Furthermore, it is demonstrated that Hjc_15-6 prefers Holliday junction oligonucleotides as compared to Y-shaped oligonucleotides as well as efficiently cleaves typical branched products from isothermal DNA amplification of both linear and circular DNA templates amplified by phi29-like DNA polymerase. The assembly of large DNA network structures was observed in real time, by transmission electron microscopy, on negative stained grids that were freshly prepared, and also on the same grids after incubation for 4 days under constant cooling. Hence, Hjc_15-6 is a promising molecular tool for efficient production of various DNA origamis that may be implemented for a wide range of applications such as within medical biomaterials, catalytic materials, molecular devices and biosensors.}}, author = {{Ahlqvist, Josefin and Tymecka-Mulik, Joanna and Burkiewicz, Katarzyna and Jasilionis, Andrius and Wallenberg, Reine and Nordberg Karlsson, Eva and Dabrowski, Slawomir}}, issn = {{1873-4863}}, language = {{eng}}, month = {{02}}, publisher = {{Elsevier}}, series = {{Journal of Biotechnology}}, title = {{DNA digestion and formation of DNA-network structures with Holliday junction-resolving enzyme Hjc_15-6 in conjunction with polymerase reactions}}, url = {{http://dx.doi.org/10.1016/j.jbiotec.2024.02.012}}, doi = {{10.1016/j.jbiotec.2024.02.012}}, year = {{2024}}, }