Determination of free and conjugated bile acids in serum of Apoe(−/−) mice fed different lingonberry fractions by UHPLC-MS
Ghaffarzadegan, Tannaz LU ; Essén, Sofia LU ; Verbrugghe, Phebe LU ; Marungruang, Nittaya LU ; Hållenius, Frida LU
; Nyman, Margareta
LU
and Sandahl, Margareta
LU
(2019)
In Scientific Reports
9.
- Abstract
- Bile acids (BAs) are known to be involved in cholesterol metabolism but interactions between the diet, BA profiles, gut microbiota and lipid metabolism have not been extensively explored. In the present study, primary and secondary BAs including their glycine and taurine-conjugated forms were quantified in serum of Apoe−/− mice by protein precipitation followed by reversed phase ultra-high-performance liquid chromatography and QTOF mass spectrometry. The mice were fed different lingonberry fractions (whole, insoluble and soluble) in a high-fat setting or cellulose in a high and low-fat setting. Serum concentrations of BAs in mice fed cellulose were higher with the high-fat diet compared to the low-fat diet (20–70%). Among the lingonberry... (More)
- Bile acids (BAs) are known to be involved in cholesterol metabolism but interactions between the diet, BA profiles, gut microbiota and lipid metabolism have not been extensively explored. In the present study, primary and secondary BAs including their glycine and taurine-conjugated forms were quantified in serum of Apoe−/− mice by protein precipitation followed by reversed phase ultra-high-performance liquid chromatography and QTOF mass spectrometry. The mice were fed different lingonberry fractions (whole, insoluble and soluble) in a high-fat setting or cellulose in a high and low-fat setting. Serum concentrations of BAs in mice fed cellulose were higher with the high-fat diet compared to the low-fat diet (20–70%). Among the lingonberry diets, the diet containing whole lingonberries had the highest concentration of chenodeoxycholic acid (CDCA), ursodeoxycholic acid (UDCA), tauro-ursodeoxycholic acid (T-UDCA), α and ω-muricholic acids (MCA) and tauro-α-MCA (T-α-MCA), and the lowest concentration of tauro-cholic acid (T-CA), deoxycholic acid (DCA) and tauro-deoxycholic acid (T-DCA). The glycine-conjugated BAs were very similar with all diets. CDCA, UDCA and α-MCA correlated positively with Bifidobacterium and Prevotella, and T-UDCA, T-α-MCA and ω-MCA with Bacteroides and Parabacteroides. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3c0455a6-3b05-4c73-91e6-ef58ed628096
- author
- Ghaffarzadegan, Tannaz
LU
; Essén, Sofia
LU
; Verbrugghe, Phebe
LU
; Marungruang, Nittaya
LU
; Hållenius, Frida
LU
; Nyman, Margareta
LU
and Sandahl, Margareta
LU
- organization
- publishing date
- 2019-03-07
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Bile acid, Lingonberry, UHPLC-MS, Mice
- in
- Scientific Reports
- volume
- 9
- article number
- 3800
- pages
- 13 pages
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:85062591393
- pmid:30846721
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-019-40272-8
- language
- English
- LU publication?
- yes
- id
- 3c0455a6-3b05-4c73-91e6-ef58ed628096
- date added to LUP
- 2019-03-08 09:07:28
- date last changed
- 2024-02-14 19:07:25
@article{3c0455a6-3b05-4c73-91e6-ef58ed628096,
abstract = {{Bile acids (BAs) are known to be involved in cholesterol metabolism but interactions between the diet, BA profiles, gut microbiota and lipid metabolism have not been extensively explored. In the present study, primary and secondary BAs including their glycine and taurine-conjugated forms were quantified in serum of Apoe−/− mice by protein precipitation followed by reversed phase ultra-high-performance liquid chromatography and QTOF mass spectrometry. The mice were fed different lingonberry fractions (whole, insoluble and soluble) in a high-fat setting or cellulose in a high and low-fat setting. Serum concentrations of BAs in mice fed cellulose were higher with the high-fat diet compared to the low-fat diet (20–70%). Among the lingonberry diets, the diet containing whole lingonberries had the highest concentration of chenodeoxycholic acid (CDCA), ursodeoxycholic acid (UDCA), tauro-ursodeoxycholic acid (T-UDCA), α and ω-muricholic acids (MCA) and tauro-α-MCA (T-α-MCA), and the lowest concentration of tauro-cholic acid (T-CA), deoxycholic acid (DCA) and tauro-deoxycholic acid (T-DCA). The glycine-conjugated BAs were very similar with all diets. CDCA, UDCA and α-MCA correlated positively with Bifidobacterium and Prevotella, and T-UDCA, T-α-MCA and ω-MCA with Bacteroides and Parabacteroides.}},
author = {{Ghaffarzadegan, Tannaz and Essén, Sofia and Verbrugghe, Phebe and Marungruang, Nittaya and Hållenius, Frida and Nyman, Margareta and Sandahl, Margareta}},
issn = {{2045-2322}},
keywords = {{Bile acid; Lingonberry; UHPLC-MS; Mice}},
language = {{eng}},
month = {{03}},
publisher = {{Nature Publishing Group}},
series = {{Scientific Reports}},
title = {{Determination of free and conjugated bile acids in serum of Apoe(−/−) mice fed different lingonberry fractions by UHPLC-MS}},
url = {{https://lup.lub.lu.se/search/files/61275162/Ghaffarzadegan_et.al_2019.pdf}},
doi = {{10.1038/s41598-019-40272-8}},
volume = {{9}},
year = {{2019}},
}