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Macromolecular characterization of high β-glucan oat lines

Majumdar, Abhinav LU ; Gil-González, Aina Belén ; Barjuan Grau, Anna LU ; Sardari, Roya R.R. LU ; Larsson, Olof LU ; Thyagarajan, Aishwarya ; Hansson, Andreas LU ; Hernández-Hernández, Oswaldo ; Olsson, Olof LU and Zambrano, José Alfredo LU (2024) In Heliyon 10(2).
Abstract

Oat (Avena sativa) is a cereal grain rich in fibers, proteins, vitamins and minerals. Oats have been linked to several health benefits, such as lowering blood cholesterol levels, counteracting cardiovascular disease and regulating blood sugar levels. This study aimed to characterize two new oat lines with high β-glucan content emanating from ethyl methyl sulphonate mutagenesis on the Lantmännen elite variety Belinda. Two of the mutated lines, and the mother variety Belinda, were profiled for β-glucan, arabinoxylan, total dietary fiber and starch composition. In addition, total lipid and protein content, amino acid composition and β-glucan molecular weights were analyzed. The high levels of β-glucan resulted in a significant increase in... (More)

Oat (Avena sativa) is a cereal grain rich in fibers, proteins, vitamins and minerals. Oats have been linked to several health benefits, such as lowering blood cholesterol levels, counteracting cardiovascular disease and regulating blood sugar levels. This study aimed to characterize two new oat lines with high β-glucan content emanating from ethyl methyl sulphonate mutagenesis on the Lantmännen elite variety Belinda. Two of the mutated lines, and the mother variety Belinda, were profiled for β-glucan, arabinoxylan, total dietary fiber and starch composition. In addition, total lipid and protein content, amino acid composition and β-glucan molecular weights were analyzed. The high levels of β-glucan resulted in a significant increase in total dietary fiber, but no correlation could be established between higher or lower levels of the assayed macromolecules, i.e., between arabinoxylan-, starch-, lipid- or protein levels in the mutated lines compared to the reference. The results indicate separate biosynthetic pathways for β-glucans and other macromolecules and an independent regulation of the different polysaccharides studied. Therefore, ethyl methyl sulphonate mutagenesis can be used to increase levels of multiple macromolecules in the same line.

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; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Amino acid analysis, Avena sativa, Dietary fibers, Macromolecular quantifications, Molecular weight determination, β-Glucan
in
Heliyon
volume
10
issue
2
article number
e24552
publisher
Elsevier
external identifiers
  • scopus:85183039329
  • pmid:38312573
ISSN
2405-8440
DOI
10.1016/j.heliyon.2024.e24552
language
English
LU publication?
yes
id
402bc79e-0cf6-4482-900b-5b20550594ce
date added to LUP
2024-04-10 14:17:44
date last changed
2024-06-19 22:19:56
@article{402bc79e-0cf6-4482-900b-5b20550594ce,
  abstract     = {{<p>Oat (Avena sativa) is a cereal grain rich in fibers, proteins, vitamins and minerals. Oats have been linked to several health benefits, such as lowering blood cholesterol levels, counteracting cardiovascular disease and regulating blood sugar levels. This study aimed to characterize two new oat lines with high β-glucan content emanating from ethyl methyl sulphonate mutagenesis on the Lantmännen elite variety Belinda. Two of the mutated lines, and the mother variety Belinda, were profiled for β-glucan, arabinoxylan, total dietary fiber and starch composition. In addition, total lipid and protein content, amino acid composition and β-glucan molecular weights were analyzed. The high levels of β-glucan resulted in a significant increase in total dietary fiber, but no correlation could be established between higher or lower levels of the assayed macromolecules, i.e., between arabinoxylan-, starch-, lipid- or protein levels in the mutated lines compared to the reference. The results indicate separate biosynthetic pathways for β-glucans and other macromolecules and an independent regulation of the different polysaccharides studied. Therefore, ethyl methyl sulphonate mutagenesis can be used to increase levels of multiple macromolecules in the same line.</p>}},
  author       = {{Majumdar, Abhinav and Gil-González, Aina Belén and Barjuan Grau, Anna and Sardari, Roya R.R. and Larsson, Olof and Thyagarajan, Aishwarya and Hansson, Andreas and Hernández-Hernández, Oswaldo and Olsson, Olof and Zambrano, José Alfredo}},
  issn         = {{2405-8440}},
  keywords     = {{Amino acid analysis; Avena sativa; Dietary fibers; Macromolecular quantifications; Molecular weight determination; β-Glucan}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{2}},
  publisher    = {{Elsevier}},
  series       = {{Heliyon}},
  title        = {{Macromolecular characterization of high β-glucan oat lines}},
  url          = {{http://dx.doi.org/10.1016/j.heliyon.2024.e24552}},
  doi          = {{10.1016/j.heliyon.2024.e24552}},
  volume       = {{10}},
  year         = {{2024}},
}