Macromolecular characterization of high β-glucan oat lines
(2024) In Heliyon 10(2).- Abstract
Oat (Avena sativa) is a cereal grain rich in fibers, proteins, vitamins and minerals. Oats have been linked to several health benefits, such as lowering blood cholesterol levels, counteracting cardiovascular disease and regulating blood sugar levels. This study aimed to characterize two new oat lines with high β-glucan content emanating from ethyl methyl sulphonate mutagenesis on the Lantmännen elite variety Belinda. Two of the mutated lines, and the mother variety Belinda, were profiled for β-glucan, arabinoxylan, total dietary fiber and starch composition. In addition, total lipid and protein content, amino acid composition and β-glucan molecular weights were analyzed. The high levels of β-glucan resulted in a significant increase in... (More)
Oat (Avena sativa) is a cereal grain rich in fibers, proteins, vitamins and minerals. Oats have been linked to several health benefits, such as lowering blood cholesterol levels, counteracting cardiovascular disease and regulating blood sugar levels. This study aimed to characterize two new oat lines with high β-glucan content emanating from ethyl methyl sulphonate mutagenesis on the Lantmännen elite variety Belinda. Two of the mutated lines, and the mother variety Belinda, were profiled for β-glucan, arabinoxylan, total dietary fiber and starch composition. In addition, total lipid and protein content, amino acid composition and β-glucan molecular weights were analyzed. The high levels of β-glucan resulted in a significant increase in total dietary fiber, but no correlation could be established between higher or lower levels of the assayed macromolecules, i.e., between arabinoxylan-, starch-, lipid- or protein levels in the mutated lines compared to the reference. The results indicate separate biosynthetic pathways for β-glucans and other macromolecules and an independent regulation of the different polysaccharides studied. Therefore, ethyl methyl sulphonate mutagenesis can be used to increase levels of multiple macromolecules in the same line.
(Less)
- author
- Majumdar, Abhinav LU ; Gil-González, Aina Belén ; Barjuan Grau, Anna LU ; Sardari, Roya R.R. LU ; Larsson, Olof LU ; Thyagarajan, Aishwarya ; Hansson, Andreas LU ; Hernández-Hernández, Oswaldo ; Olsson, Olof LU and Zambrano, José Alfredo LU
- organization
- publishing date
- 2024-01-30
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Amino acid analysis, Avena sativa, Dietary fibers, Macromolecular quantifications, Molecular weight determination, β-Glucan
- in
- Heliyon
- volume
- 10
- issue
- 2
- article number
- e24552
- publisher
- Elsevier
- external identifiers
-
- pmid:38312573
- scopus:85183039329
- ISSN
- 2405-8440
- DOI
- 10.1016/j.heliyon.2024.e24552
- language
- English
- LU publication?
- yes
- id
- 402bc79e-0cf6-4482-900b-5b20550594ce
- date added to LUP
- 2024-04-10 14:17:44
- date last changed
- 2024-04-24 17:13:52
@article{402bc79e-0cf6-4482-900b-5b20550594ce,
abstract = {{<p>Oat (Avena sativa) is a cereal grain rich in fibers, proteins, vitamins and minerals. Oats have been linked to several health benefits, such as lowering blood cholesterol levels, counteracting cardiovascular disease and regulating blood sugar levels. This study aimed to characterize two new oat lines with high β-glucan content emanating from ethyl methyl sulphonate mutagenesis on the Lantmännen elite variety Belinda. Two of the mutated lines, and the mother variety Belinda, were profiled for β-glucan, arabinoxylan, total dietary fiber and starch composition. In addition, total lipid and protein content, amino acid composition and β-glucan molecular weights were analyzed. The high levels of β-glucan resulted in a significant increase in total dietary fiber, but no correlation could be established between higher or lower levels of the assayed macromolecules, i.e., between arabinoxylan-, starch-, lipid- or protein levels in the mutated lines compared to the reference. The results indicate separate biosynthetic pathways for β-glucans and other macromolecules and an independent regulation of the different polysaccharides studied. Therefore, ethyl methyl sulphonate mutagenesis can be used to increase levels of multiple macromolecules in the same line.</p>}},
author = {{Majumdar, Abhinav and Gil-González, Aina Belén and Barjuan Grau, Anna and Sardari, Roya R.R. and Larsson, Olof and Thyagarajan, Aishwarya and Hansson, Andreas and Hernández-Hernández, Oswaldo and Olsson, Olof and Zambrano, José Alfredo}},
issn = {{2405-8440}},
keywords = {{Amino acid analysis; Avena sativa; Dietary fibers; Macromolecular quantifications; Molecular weight determination; β-Glucan}},
language = {{eng}},
month = {{01}},
number = {{2}},
publisher = {{Elsevier}},
series = {{Heliyon}},
title = {{Macromolecular characterization of high β-glucan oat lines}},
url = {{http://dx.doi.org/10.1016/j.heliyon.2024.e24552}},
doi = {{10.1016/j.heliyon.2024.e24552}},
volume = {{10}},
year = {{2024}},
}