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Different expression levels of glycans on leukemic cells—a novel screening method with molecularly imprinted polymers (MIP) targeting sialic acid

El-Schich, Zahra; Abdullah, Mohammad; Shinde, Sudhirkumar; Dizeyi, Nishtman LU ; Rosén, Anders; Sellergren, Börje and Wingren, Anette Gjörloff LU (2016) In Tumor Biology 37(10). p.13763-13768
Abstract

Sialic acid (SA) is normally expressed on the cell membranes and is located at the terminal position of the sugar chains. SA plays an important role for regulation of the innate immunity, function as markers of the cells and can be recognized by a variety of receptors. Interestingly, the level of SA expression is increased on metastatic cancer cells. The availability of specific antibodies against SA is limited and, therefore, biomarker tools for detection of SA are lacking. We have recently presented a novel method for specific fluorescence labeling of SA molecular imprinted polymers (MIP). Here, we have performed an extended screening of SA expression by using SA-MIP and included four different chronic lymphocytic leukemia (CLL) cell... (More)

Sialic acid (SA) is normally expressed on the cell membranes and is located at the terminal position of the sugar chains. SA plays an important role for regulation of the innate immunity, function as markers of the cells and can be recognized by a variety of receptors. Interestingly, the level of SA expression is increased on metastatic cancer cells. The availability of specific antibodies against SA is limited and, therefore, biomarker tools for detection of SA are lacking. We have recently presented a novel method for specific fluorescence labeling of SA molecular imprinted polymers (MIP). Here, we have performed an extended screening of SA expression by using SA-MIP and included four different chronic lymphocytic leukemia (CLL) cell lines, conveniently analyzed by flow cytometry and fluorescence microscopy. SA expression was detected in four cell lines at different levels, and the SA expression were verified with lectin-FITC. These results show that SA-MIP can be used as a plastic antibody for detection of SA using both flow cytometry and fluorescence microscopy. We suggest that SA-MIP can be used for screening of different tumor cells of various stages, including CLL cells.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Chronic lymphocytic leukemia, Lectin, Molecular imprinting polymers, Sialic acid
in
Tumor Biology
volume
37
issue
10
pages
13763 - 13768
publisher
Springer
external identifiers
  • scopus:84980037288
  • wos:000387538700076
ISSN
1010-4283
DOI
10.1007/s13277-016-5280-y
language
English
LU publication?
yes
id
40aff94a-dae8-4e04-9e8b-71be357c8ab0
date added to LUP
2016-12-19 13:17:32
date last changed
2017-09-18 11:32:13
@article{40aff94a-dae8-4e04-9e8b-71be357c8ab0,
  abstract     = {<p>Sialic acid (SA) is normally expressed on the cell membranes and is located at the terminal position of the sugar chains. SA plays an important role for regulation of the innate immunity, function as markers of the cells and can be recognized by a variety of receptors. Interestingly, the level of SA expression is increased on metastatic cancer cells. The availability of specific antibodies against SA is limited and, therefore, biomarker tools for detection of SA are lacking. We have recently presented a novel method for specific fluorescence labeling of SA molecular imprinted polymers (MIP). Here, we have performed an extended screening of SA expression by using SA-MIP and included four different chronic lymphocytic leukemia (CLL) cell lines, conveniently analyzed by flow cytometry and fluorescence microscopy. SA expression was detected in four cell lines at different levels, and the SA expression were verified with lectin-FITC. These results show that SA-MIP can be used as a plastic antibody for detection of SA using both flow cytometry and fluorescence microscopy. We suggest that SA-MIP can be used for screening of different tumor cells of various stages, including CLL cells.</p>},
  author       = {El-Schich, Zahra and Abdullah, Mohammad and Shinde, Sudhirkumar and Dizeyi, Nishtman and Rosén, Anders and Sellergren, Börje and Wingren, Anette Gjörloff},
  issn         = {1010-4283},
  keyword      = {Chronic lymphocytic leukemia,Lectin,Molecular imprinting polymers,Sialic acid},
  language     = {eng},
  number       = {10},
  pages        = {13763--13768},
  publisher    = {Springer},
  series       = {Tumor Biology},
  title        = {Different expression levels of glycans on leukemic cells—a novel screening method with molecularly imprinted polymers (MIP) targeting sialic acid},
  url          = {http://dx.doi.org/10.1007/s13277-016-5280-y},
  volume       = {37},
  year         = {2016},
}