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Variations of wettability and protein adsorption on solid siliceous carriers grafted with poly(N-isopropylacrylamide)

Ivanov, Alexander LU ; Ekeroth, J ; Nilsson, Lars LU ; Mattiasson, Bo LU ; Bergenståhl, Björn LU and Galaev, Igor LU (2006) In Journal of Colloid and Interface Science 296(2). p.538-544
Abstract
Poly(N-isopropylacrylamide), a thermally responsive polymer, was end-grafted to mercaptopropyl derivatives of silica gel, plane glass sheets and glass capillary tubing by free radical polymerization of the monomer in 1,4-dioxane at 100 degrees C. The polymer monolayer attached to the glass carriers provided them with thermally controlled wettability registered by two independent methods: direct measurements of the water contact angle and capillary rise. The water Contact angle changed from 54 +/- 3 degrees to 68 +/- 3 degrees in the temperature range from 20 to 50 degrees C. The polymer grafting to silica gel (pore diameter 100 A, particle size 5 pm) resulted in 15-30-fold reduction in protein adsorption on the carrier at 35 degrees C.... (More)
Poly(N-isopropylacrylamide), a thermally responsive polymer, was end-grafted to mercaptopropyl derivatives of silica gel, plane glass sheets and glass capillary tubing by free radical polymerization of the monomer in 1,4-dioxane at 100 degrees C. The polymer monolayer attached to the glass carriers provided them with thermally controlled wettability registered by two independent methods: direct measurements of the water contact angle and capillary rise. The water Contact angle changed from 54 +/- 3 degrees to 68 +/- 3 degrees in the temperature range from 20 to 50 degrees C. The polymer grafting to silica gel (pore diameter 100 A, particle size 5 pm) resulted in 15-30-fold reduction in protein adsorption on the carrier at 35 degrees C. Adsorption isotherms of myoglobin indicate completely different characters of the protein adsorption to silica gel and its polyNIPAM-grafted derivative. Cooling of the grafted carrier containing adsorbed myoglobin to 9 degrees C led to a partial release of the protein to the contacting solution, whereas heating of the system to 35 degrees C resulted in reversible binding of the protein. Adsorption of myoglobin on polyNIPAM-coated silica was ca. 2-fold higher at 35 than at 9 degrees C, most probably (c) 2005 Elsevier Inc. All rights reserved. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
thermoresponsive polymers, silica, grafting, protein adsorption
in
Journal of Colloid and Interface Science
volume
296
issue
2
pages
538 - 544
publisher
Elsevier
external identifiers
  • wos:000236424600019
  • scopus:33644996060
  • pmid:16243347
ISSN
1095-7103
DOI
10.1016/j.jcis.2005.09.064
language
English
LU publication?
yes
id
e1d2c3b1-6022-4100-b510-38324d166687 (old id 414790)
date added to LUP
2016-04-01 12:18:36
date last changed
2021-08-04 01:01:15
@article{e1d2c3b1-6022-4100-b510-38324d166687,
  abstract     = {Poly(N-isopropylacrylamide), a thermally responsive polymer, was end-grafted to mercaptopropyl derivatives of silica gel, plane glass sheets and glass capillary tubing by free radical polymerization of the monomer in 1,4-dioxane at 100 degrees C. The polymer monolayer attached to the glass carriers provided them with thermally controlled wettability registered by two independent methods: direct measurements of the water contact angle and capillary rise. The water Contact angle changed from 54 +/- 3 degrees to 68 +/- 3 degrees in the temperature range from 20 to 50 degrees C. The polymer grafting to silica gel (pore diameter 100 A, particle size 5 pm) resulted in 15-30-fold reduction in protein adsorption on the carrier at 35 degrees C. Adsorption isotherms of myoglobin indicate completely different characters of the protein adsorption to silica gel and its polyNIPAM-grafted derivative. Cooling of the grafted carrier containing adsorbed myoglobin to 9 degrees C led to a partial release of the protein to the contacting solution, whereas heating of the system to 35 degrees C resulted in reversible binding of the protein. Adsorption of myoglobin on polyNIPAM-coated silica was ca. 2-fold higher at 35 than at 9 degrees C, most probably (c) 2005 Elsevier Inc. All rights reserved.},
  author       = {Ivanov, Alexander and Ekeroth, J and Nilsson, Lars and Mattiasson, Bo and Bergenståhl, Björn and Galaev, Igor},
  issn         = {1095-7103},
  language     = {eng},
  number       = {2},
  pages        = {538--544},
  publisher    = {Elsevier},
  series       = {Journal of Colloid and Interface Science},
  title        = {Variations of wettability and protein adsorption on solid siliceous carriers grafted with poly(N-isopropylacrylamide)},
  url          = {http://dx.doi.org/10.1016/j.jcis.2005.09.064},
  doi          = {10.1016/j.jcis.2005.09.064},
  volume       = {296},
  year         = {2006},
}