Evaluation of multiple oxidation products for monitoring effects of antioxidants in Fenton oxidation of 2 '-deoxyguanosine
(2006) In Journal of Agricultural and Food Chemistry 54(6). p.2350-2358- Abstract
- The objective of this study was to investigate the influence of the two antioxidants, ascorbic acid and (+)catechin, on the oxidation of 2'-deoxyguanosine (dG), using an iron-mediated Fenton reaction. The oxidation products 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8,5'-cyclo-2'-deoxyguanosine, together with the secondary oxidation products guanidinohydantoin and dehydroguanidinohydantoin, were identified and quantified through the use of an LC-MS/MS system. The results obtained showed that catechin inhibited the oxidation better than ascorbic acid did, indicating that the chelating ability of catechin rather than the radical scavenging mechanism alone is vital for the observed antioxidative efficiency. The correlation between the... (More)
- The objective of this study was to investigate the influence of the two antioxidants, ascorbic acid and (+)catechin, on the oxidation of 2'-deoxyguanosine (dG), using an iron-mediated Fenton reaction. The oxidation products 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8,5'-cyclo-2'-deoxyguanosine, together with the secondary oxidation products guanidinohydantoin and dehydroguanidinohydantoin, were identified and quantified through the use of an LC-MS/MS system. The results obtained showed that catechin inhibited the oxidation better than ascorbic acid did, indicating that the chelating ability of catechin rather than the radical scavenging mechanism alone is vital for the observed antioxidative efficiency. The correlation between the different oxidation products was found to be quite low, primarily because of the instability of 8-oxodG, making it prone to further oxidation. This led to apparent anti- and pro-oxidative results being obtained, emphasizing the potential problems in evaluating oxidative stress, by use of a single marker. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/415179
- author
- Grey, Carl LU and Adlercreutz, Patrick LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- ascorbic acid, Fenton reaction, oxidation, 2 '-deoxyguanosine, catechin
- in
- Journal of Agricultural and Food Chemistry
- volume
- 54
- issue
- 6
- pages
- 2350 - 2358
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000236227100057
- pmid:16536618
- scopus:33645792170
- ISSN
- 0021-8561
- DOI
- 10.1021/jf052202q
- language
- English
- LU publication?
- yes
- id
- 0a0114f5-b7e0-4ccc-a508-dc26a47e2988 (old id 415179)
- date added to LUP
- 2016-04-01 12:26:51
- date last changed
- 2024-10-09 10:47:37
@article{0a0114f5-b7e0-4ccc-a508-dc26a47e2988, abstract = {{The objective of this study was to investigate the influence of the two antioxidants, ascorbic acid and (+)catechin, on the oxidation of 2'-deoxyguanosine (dG), using an iron-mediated Fenton reaction. The oxidation products 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8,5'-cyclo-2'-deoxyguanosine, together with the secondary oxidation products guanidinohydantoin and dehydroguanidinohydantoin, were identified and quantified through the use of an LC-MS/MS system. The results obtained showed that catechin inhibited the oxidation better than ascorbic acid did, indicating that the chelating ability of catechin rather than the radical scavenging mechanism alone is vital for the observed antioxidative efficiency. The correlation between the different oxidation products was found to be quite low, primarily because of the instability of 8-oxodG, making it prone to further oxidation. This led to apparent anti- and pro-oxidative results being obtained, emphasizing the potential problems in evaluating oxidative stress, by use of a single marker.}}, author = {{Grey, Carl and Adlercreutz, Patrick}}, issn = {{0021-8561}}, keywords = {{ascorbic acid; Fenton reaction; oxidation; 2 '-deoxyguanosine; catechin}}, language = {{eng}}, number = {{6}}, pages = {{2350--2358}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Journal of Agricultural and Food Chemistry}}, title = {{Evaluation of multiple oxidation products for monitoring effects of antioxidants in Fenton oxidation of 2 '-deoxyguanosine}}, url = {{http://dx.doi.org/10.1021/jf052202q}}, doi = {{10.1021/jf052202q}}, volume = {{54}}, year = {{2006}}, }