Short and long-range cyclic patterns in flows of DNA solutions in microfluidic obstacle arrays
(2023) In Lab on a Chip 23. p.1779-1793- Abstract
We observe regular patterns emerging across multiple length scales with high-concentration DNA solutions in microfluidic pillar arrays at low Reynolds numbers and high Deborah numbers. Interacting vortices between pillars lead to long-range order in the form of large travelling waves consisting of DNA at high concentration and extension. Waves are formed in quadratic arrays of pillars, while randomizing the position of the pillar in each unit cell of a quadratic array leads to suppression of the long-range patterns. We find that concentrations exceeding the overlap concentration of the DNA enables the waves, and exploring the behavior of the waves as a function of flow rate, buffer composition, concentration and molecular length, we... (More)
We observe regular patterns emerging across multiple length scales with high-concentration DNA solutions in microfluidic pillar arrays at low Reynolds numbers and high Deborah numbers. Interacting vortices between pillars lead to long-range order in the form of large travelling waves consisting of DNA at high concentration and extension. Waves are formed in quadratic arrays of pillars, while randomizing the position of the pillar in each unit cell of a quadratic array leads to suppression of the long-range patterns. We find that concentrations exceeding the overlap concentration of the DNA enables the waves, and exploring the behavior of the waves as a function of flow rate, buffer composition, concentration and molecular length, we identify elastic effects as central to the origin of the waves. Our work may not only help increase the low throughput that often limits sample processing in microfluidics, it may also provide a platform for further studies of the underlying viscoelastic mechanisms.
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- author
- Ström, Oskar E. LU ; Beech, Jason P. LU and Tegenfeldt, Jonas O. LU
- organization
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Lab on a Chip
- volume
- 23
- pages
- 15 pages
- publisher
- Royal Society of Chemistry
- external identifiers
-
- scopus:85148869161
- pmid:36807458
- ISSN
- 1473-0197
- DOI
- 10.1039/d2lc01051h
- language
- English
- LU publication?
- yes
- additional info
- Funding Information: This research was funded by the European Union within Horizon2020, grant number 634890 (project name BeyondSeq), EuroNanoMed (project name NanoDiaBac), by the Swedish Research council, grant number 2016-05739 and NanoLund, grant numbers p20-2019 and staff01-2020. All device processing was conducted within Lund Nano Lab. Publisher Copyright: © 2023 The Royal Society of Chemistry.
- id
- 417da42d-569e-48d1-a436-e1889a8ae26c
- date added to LUP
- 2023-03-09 19:31:08
- date last changed
- 2024-09-20 00:00:14
@article{417da42d-569e-48d1-a436-e1889a8ae26c, abstract = {{<p>We observe regular patterns emerging across multiple length scales with high-concentration DNA solutions in microfluidic pillar arrays at low Reynolds numbers and high Deborah numbers. Interacting vortices between pillars lead to long-range order in the form of large travelling waves consisting of DNA at high concentration and extension. Waves are formed in quadratic arrays of pillars, while randomizing the position of the pillar in each unit cell of a quadratic array leads to suppression of the long-range patterns. We find that concentrations exceeding the overlap concentration of the DNA enables the waves, and exploring the behavior of the waves as a function of flow rate, buffer composition, concentration and molecular length, we identify elastic effects as central to the origin of the waves. Our work may not only help increase the low throughput that often limits sample processing in microfluidics, it may also provide a platform for further studies of the underlying viscoelastic mechanisms.</p>}}, author = {{Ström, Oskar E. and Beech, Jason P. and Tegenfeldt, Jonas O.}}, issn = {{1473-0197}}, language = {{eng}}, pages = {{1779--1793}}, publisher = {{Royal Society of Chemistry}}, series = {{Lab on a Chip}}, title = {{Short and long-range cyclic patterns in flows of DNA solutions in microfluidic obstacle arrays}}, url = {{http://dx.doi.org/10.1039/d2lc01051h}}, doi = {{10.1039/d2lc01051h}}, volume = {{23}}, year = {{2023}}, }