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Identification of tumor margins using diffuse reflectance spectroscopy with an extended-wavelength spectrum in a porcine model

Dahlstrand, U LU ; Sheikh, R LU ; Nguyen, C D LU ; Hult, J LU ; Reistad, N LU and Malmsjö, M LU (2018) In Skin Research and Technology 24(4). p.667-671
Abstract

OBJECTIVE: A novel extended-wavelength diffuse reflectance spectroscopy (EWDRS) technique is being developed for future clinical non-invasive tumor margin delineation. In this study, the ability of EWDRS to identify the margins of pigmented skin lesions in an in vivo pig model was evaluated.

MATERIALS AND METHODS: Extended-wavelength diffuse reflectance spectroscopy recordings (350-1550 nm) were made on 13 pigmented skin lesions and non-pigmented skin, as a reference. The hand-held probe was swept toward the pigmented area until the signal changed, thus indicating that the margin had been identified. A needle was inserted as a marker, and tissue samples were sent for histological analysis. The distance between the EWDRS-defined... (More)

OBJECTIVE: A novel extended-wavelength diffuse reflectance spectroscopy (EWDRS) technique is being developed for future clinical non-invasive tumor margin delineation. In this study, the ability of EWDRS to identify the margins of pigmented skin lesions in an in vivo pig model was evaluated.

MATERIALS AND METHODS: Extended-wavelength diffuse reflectance spectroscopy recordings (350-1550 nm) were made on 13 pigmented skin lesions and non-pigmented skin, as a reference. The hand-held probe was swept toward the pigmented area until the signal changed, thus indicating that the margin had been identified. A needle was inserted as a marker, and tissue samples were sent for histological analysis. The distance between the EWDRS-defined border and the histological border was measured by 3 independent examiners.

RESULTS: The median difference between the EWDRS-defined border and the histological border was 70 μm toward the pigmented tissue (range: -579 to 538 μm). A Pearson correlation coefficient of .95 was obtained for the examiners.

CONCLUSIONS: Extended-wavelength diffuse reflectance spectroscopy can be used in vivo to delineate the border of pigmented skin lesions in a porcine model with high accuracy, indicating that it may be a useful tool for non-invasive tumor margin delineation in the future.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Journal Article
in
Skin Research and Technology
volume
24
issue
4
pages
5 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:85054294356
ISSN
0909-752X
DOI
10.1111/srt.12583
language
English
LU publication?
yes
id
42b93ce3-0a41-4cbf-9ae0-cabff80c9afe
date added to LUP
2018-05-22 21:18:47
date last changed
2019-02-03 05:32:42
@article{42b93ce3-0a41-4cbf-9ae0-cabff80c9afe,
  abstract     = {<p>OBJECTIVE: A novel extended-wavelength diffuse reflectance spectroscopy (EWDRS) technique is being developed for future clinical non-invasive tumor margin delineation. In this study, the ability of EWDRS to identify the margins of pigmented skin lesions in an in vivo pig model was evaluated.</p><p>MATERIALS AND METHODS: Extended-wavelength diffuse reflectance spectroscopy recordings (350-1550 nm) were made on 13 pigmented skin lesions and non-pigmented skin, as a reference. The hand-held probe was swept toward the pigmented area until the signal changed, thus indicating that the margin had been identified. A needle was inserted as a marker, and tissue samples were sent for histological analysis. The distance between the EWDRS-defined border and the histological border was measured by 3 independent examiners.</p><p>RESULTS: The median difference between the EWDRS-defined border and the histological border was 70 μm toward the pigmented tissue (range: -579 to 538 μm). A Pearson correlation coefficient of .95 was obtained for the examiners.</p><p>CONCLUSIONS: Extended-wavelength diffuse reflectance spectroscopy can be used in vivo to delineate the border of pigmented skin lesions in a porcine model with high accuracy, indicating that it may be a useful tool for non-invasive tumor margin delineation in the future.</p>},
  author       = {Dahlstrand, U and Sheikh, R and Nguyen, C D and Hult, J and Reistad, N and Malmsjö, M},
  issn         = {0909-752X},
  keyword      = {Journal Article},
  language     = {eng},
  number       = {4},
  pages        = {667--671},
  publisher    = {Wiley-Blackwell},
  series       = {Skin Research and Technology},
  title        = {Identification of tumor margins using diffuse reflectance spectroscopy with an extended-wavelength spectrum in a porcine model},
  url          = {http://dx.doi.org/10.1111/srt.12583},
  volume       = {24},
  year         = {2018},
}