Advanced

Straightforward rapid spectrophotometric quantification of total cyanogenic glycosides in fresh and processed cassava products.

Tivana, Lucas LU ; Da Cruz Francisco, Jose; Zelder, Felix; Bergenståhl, Björn LU and Dejmek, Petr LU (2014) In Food Chemistry 158. p.20-27
Abstract
In this study, we extend pioneering studies and demonstrate straightforward applicability of the corrin-based chemosensor, aquacyanocobyrinic acid (ACCA), for the instantaneous detection and rapid quantification of endogenous cyanide in fresh and processed cassava roots. Hydrolytically liberated endogenous cyanide from cyanogenic glycosides (CNp) reacts with ACCA to form dicyanocobyrinic acid (DCCA), accompanied by a change of colour from orange to violet. The method was successfully tested on various cassava samples containing between 6 and 200mgequiv.HCN/kg as verified with isonicotinate/1,3-dimethylbarbiturate as an independent method. The affinity of ACCA sensor to cyanide is high, coordination occurs fast and the colorimetric response... (More)
In this study, we extend pioneering studies and demonstrate straightforward applicability of the corrin-based chemosensor, aquacyanocobyrinic acid (ACCA), for the instantaneous detection and rapid quantification of endogenous cyanide in fresh and processed cassava roots. Hydrolytically liberated endogenous cyanide from cyanogenic glycosides (CNp) reacts with ACCA to form dicyanocobyrinic acid (DCCA), accompanied by a change of colour from orange to violet. The method was successfully tested on various cassava samples containing between 6 and 200mgequiv.HCN/kg as verified with isonicotinate/1,3-dimethylbarbiturate as an independent method. The affinity of ACCA sensor to cyanide is high, coordination occurs fast and the colorimetric response can therefore be instantaneously monitored with spectrophotometric methods. Direct applications of the sensor without need of extensive and laborious extraction processes are demonstrated in water-extracted samples, in acid-extracted samples, and directly on juice drops. ACCA showed high precision with a standard deviation (STDV) between 0.03 and 0.06 and high accuracy (93-96%). Overall, the ACCA procedure is straightforward, safe and easily performed. In a proof-of-concept study, rapid screening of ten samples within 20min has been tested. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Food Chemistry
volume
158
pages
20 - 27
publisher
Elsevier
external identifiers
  • pmid:24731309
  • wos:000335805600004
  • scopus:84896501186
ISSN
1873-7072
DOI
10.1016/j.foodchem.2014.02.066
language
English
LU publication?
yes
id
408c4935-1945-4344-abea-5feb2690509e (old id 4430068)
date added to LUP
2014-05-07 17:50:15
date last changed
2017-09-10 04:17:27
@article{408c4935-1945-4344-abea-5feb2690509e,
  abstract     = {In this study, we extend pioneering studies and demonstrate straightforward applicability of the corrin-based chemosensor, aquacyanocobyrinic acid (ACCA), for the instantaneous detection and rapid quantification of endogenous cyanide in fresh and processed cassava roots. Hydrolytically liberated endogenous cyanide from cyanogenic glycosides (CNp) reacts with ACCA to form dicyanocobyrinic acid (DCCA), accompanied by a change of colour from orange to violet. The method was successfully tested on various cassava samples containing between 6 and 200mgequiv.HCN/kg as verified with isonicotinate/1,3-dimethylbarbiturate as an independent method. The affinity of ACCA sensor to cyanide is high, coordination occurs fast and the colorimetric response can therefore be instantaneously monitored with spectrophotometric methods. Direct applications of the sensor without need of extensive and laborious extraction processes are demonstrated in water-extracted samples, in acid-extracted samples, and directly on juice drops. ACCA showed high precision with a standard deviation (STDV) between 0.03 and 0.06 and high accuracy (93-96%). Overall, the ACCA procedure is straightforward, safe and easily performed. In a proof-of-concept study, rapid screening of ten samples within 20min has been tested.},
  author       = {Tivana, Lucas and Da Cruz Francisco, Jose and Zelder, Felix and Bergenståhl, Björn and Dejmek, Petr},
  issn         = {1873-7072},
  language     = {eng},
  pages        = {20--27},
  publisher    = {Elsevier},
  series       = {Food Chemistry},
  title        = {Straightforward rapid spectrophotometric quantification of total cyanogenic glycosides in fresh and processed cassava products.},
  url          = {http://dx.doi.org/10.1016/j.foodchem.2014.02.066},
  volume       = {158},
  year         = {2014},
}