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Fluorescently labeled xylosides offer insight into the biosynthetic pathways of glycosaminoglycans

Mastio, Roberto LU ; Frankel Willén, Daniel LU ; Söderlund, Zackarias LU orcid ; Westergren-Thorsson, Gunilla LU ; Manner, Sophie LU ; Tykesson, Emil LU orcid and Ellervik, Ulf LU (2021) In RSC Advances 11(60). p.38283-38292
Abstract

Five novel xylosides tagged with the fluorescent probe Pacific Blue™ were synthesized and found to act as substrates for β4GalT7, a bottleneck enzyme in the biosynthetic pathways leading to glycosaminoglycans. By confocal microscopy of A549 cells, we showed that the xylosides were taken up by the cells, but did not enter the Golgi apparatus where most of the glycosaminoglycan biosynthesis occurs. Instead, after a possible double galactosylation by β4GalT7 and β3GalT6, the biosynthesis was terminated. We hypothesize this is due to the charge of the fluorescent probe, which is required for fluorescent ability and stability under physiological conditions.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
RSC Advances
volume
11
issue
60
pages
10 pages
publisher
Royal Society of Chemistry
external identifiers
  • scopus:85119918793
  • pmid:35498069
ISSN
2046-2069
DOI
10.1039/d1ra06320k
language
English
LU publication?
yes
additional info
This journal is © The Royal Society of Chemistry.
id
46822025-20a6-47d1-91a9-b96463ee8ead
date added to LUP
2023-08-11 08:09:23
date last changed
2024-03-22 23:04:16
@article{46822025-20a6-47d1-91a9-b96463ee8ead,
  abstract     = {{<p>Five novel xylosides tagged with the fluorescent probe Pacific Blue™ were synthesized and found to act as substrates for β4GalT7, a bottleneck enzyme in the biosynthetic pathways leading to glycosaminoglycans. By confocal microscopy of A549 cells, we showed that the xylosides were taken up by the cells, but did not enter the Golgi apparatus where most of the glycosaminoglycan biosynthesis occurs. Instead, after a possible double galactosylation by β4GalT7 and β3GalT6, the biosynthesis was terminated. We hypothesize this is due to the charge of the fluorescent probe, which is required for fluorescent ability and stability under physiological conditions.</p>}},
  author       = {{Mastio, Roberto and Frankel Willén, Daniel and Söderlund, Zackarias and Westergren-Thorsson, Gunilla and Manner, Sophie and Tykesson, Emil and Ellervik, Ulf}},
  issn         = {{2046-2069}},
  language     = {{eng}},
  month        = {{11}},
  number       = {{60}},
  pages        = {{38283--38292}},
  publisher    = {{Royal Society of Chemistry}},
  series       = {{RSC Advances}},
  title        = {{Fluorescently labeled xylosides offer insight into the biosynthetic pathways of glycosaminoglycans}},
  url          = {{http://dx.doi.org/10.1039/d1ra06320k}},
  doi          = {{10.1039/d1ra06320k}},
  volume       = {{11}},
  year         = {{2021}},
}