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Production of arabinoxylan-oligosaccharide mixtures of varying composition from rye bran by combination of process conditions and type of xylanase.

Falck, Peter LU ; Aronsson, Anna LU ; Grey, Carl LU ; Stålbrand, Henrik LU ; Nordberg Karlsson, Eva LU and Adlercreutz, Patrick LU (2014) In Bioresource Technology 174. p.118-125
Abstract
The aim was to study arabinoxylan-oligosaccharide production from rye bran using heat pretreatment and enzymatic hydrolysis. Due to the potential application in foods, the purity of arabinoxylan was also assessed. Rye bran was heat pretreated to improve xylanase-catalyzed hydrolysis of arabinoxylan into arabinoxylan-oligosaccharides. Enzymatic removal of starch and proteins before or after heat pretreatment increased the purity, although at lower yield. The most attractive process resulted in 62% (w/w) arabinoxylan content after ethanol precipitation. Using xylanases from two glycoside hydrolase families (RmXyn10A from GH10 and Pentopan Mono BG from GH11), different mixtures of unsubstituted and arabinose-substituted xylooligosaccharides... (More)
The aim was to study arabinoxylan-oligosaccharide production from rye bran using heat pretreatment and enzymatic hydrolysis. Due to the potential application in foods, the purity of arabinoxylan was also assessed. Rye bran was heat pretreated to improve xylanase-catalyzed hydrolysis of arabinoxylan into arabinoxylan-oligosaccharides. Enzymatic removal of starch and proteins before or after heat pretreatment increased the purity, although at lower yield. The most attractive process resulted in 62% (w/w) arabinoxylan content after ethanol precipitation. Using xylanases from two glycoside hydrolase families (RmXyn10A from GH10 and Pentopan Mono BG from GH11), different mixtures of unsubstituted and arabinose-substituted xylooligosaccharides were produced. GH10 gave a higher yield of short oligosaccharides (60% w/w) with xylobiose as the main product; xylobiose and xylotriose were the main products with GH11 (40% w/w). Thus, heat pretreatment combined with enzymatic hydrolysis can be used to produce arabinoxylan-oligosaccharides from rye bran that are potentially useful in functional foods. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Heat pretreatment, Xylooligosaccharides, Arabinoxylooligosaccharides, GH10, GH11
in
Bioresource Technology
volume
174
pages
118 - 125
publisher
Elsevier
external identifiers
  • pmid:25463790
  • wos:000345014900016
  • scopus:84908317360
ISSN
1873-2976
DOI
10.1016/j.biortech.2014.09.139
language
English
LU publication?
yes
id
4a4ed6df-540e-4aff-b3bb-69fdebc382c0 (old id 4755295)
date added to LUP
2014-11-07 16:57:31
date last changed
2017-10-01 03:11:35
@article{4a4ed6df-540e-4aff-b3bb-69fdebc382c0,
  abstract     = {The aim was to study arabinoxylan-oligosaccharide production from rye bran using heat pretreatment and enzymatic hydrolysis. Due to the potential application in foods, the purity of arabinoxylan was also assessed. Rye bran was heat pretreated to improve xylanase-catalyzed hydrolysis of arabinoxylan into arabinoxylan-oligosaccharides. Enzymatic removal of starch and proteins before or after heat pretreatment increased the purity, although at lower yield. The most attractive process resulted in 62% (w/w) arabinoxylan content after ethanol precipitation. Using xylanases from two glycoside hydrolase families (RmXyn10A from GH10 and Pentopan Mono BG from GH11), different mixtures of unsubstituted and arabinose-substituted xylooligosaccharides were produced. GH10 gave a higher yield of short oligosaccharides (60% w/w) with xylobiose as the main product; xylobiose and xylotriose were the main products with GH11 (40% w/w). Thus, heat pretreatment combined with enzymatic hydrolysis can be used to produce arabinoxylan-oligosaccharides from rye bran that are potentially useful in functional foods.},
  author       = {Falck, Peter and Aronsson, Anna and Grey, Carl and Stålbrand, Henrik and Nordberg Karlsson, Eva and Adlercreutz, Patrick},
  issn         = {1873-2976},
  keyword      = {Heat pretreatment,Xylooligosaccharides,Arabinoxylooligosaccharides,GH10,GH11},
  language     = {eng},
  pages        = {118--125},
  publisher    = {Elsevier},
  series       = {Bioresource Technology},
  title        = {Production of arabinoxylan-oligosaccharide mixtures of varying composition from rye bran by combination of process conditions and type of xylanase.},
  url          = {http://dx.doi.org/10.1016/j.biortech.2014.09.139},
  volume       = {174},
  year         = {2014},
}