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Effects of barley variety, dietary fiber and β-glucan content on bile acid composition in cecum of rats fed low- and high-fat diets

Ghaffarzadegan, Tannaz LU ; Zhong, Yadong LU ; Fåk Hållenius, Frida and Nyman, Margareta LU (2018) In Journal of Nutritional Biochemistry 53. p.104-110
Abstract

Diet-induced obesity and insulin resistance have been linked to changes in bile acid (BA) profiles, which in turn are highly dependent on the dietary composition and activity of the gut microbiota. The objective of the present study was to investigate whether the type and level of fiber had an effect on cecal BA composition when included in low- and high-fat diets. Groups of rats were fed two barley varieties, which resulted in three test diets containing three levels of β-glucans and two levels of dietary fiber. BAs were preconcentrated using hollow fiber liquid-phase microextraction and quantified by gas chromatography. The amount of the secondary BAs, lithocholic-, deoxycholic- and hyodexycholic acids was generally higher in groups... (More)

Diet-induced obesity and insulin resistance have been linked to changes in bile acid (BA) profiles, which in turn are highly dependent on the dietary composition and activity of the gut microbiota. The objective of the present study was to investigate whether the type and level of fiber had an effect on cecal BA composition when included in low- and high-fat diets. Groups of rats were fed two barley varieties, which resulted in three test diets containing three levels of β-glucans and two levels of dietary fiber. BAs were preconcentrated using hollow fiber liquid-phase microextraction and quantified by gas chromatography. The amount of the secondary BAs, lithocholic-, deoxycholic- and hyodexycholic acids was generally higher in groups fed high-fat diets compared with corresponding acids in groups fed low-fat diets (P<.05). In contrast, most of the primary and the secondary BAs, ursodeoxycholic acid and β- and ω-muricholic acids, were two to five times higher (P<.05) in groups fed low-fat diets than in groups fed high-fat diets. This was particularly true for groups fed the highest level of β-glucans and in some cases also the medium level. The BA profile in the gut was strongly dependent on the amount and type of dietary fiber in the diet, which may be useful in the prevention/treatment of diseases associated with changes in BA profiles.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Barley, Bile acids, Cellulose, Dietary fiber, Gas chromatography, β-Glucans
in
Journal of Nutritional Biochemistry
volume
53
pages
7 pages
publisher
Elsevier
external identifiers
  • scopus:85036555668
ISSN
0955-2863
DOI
language
English
LU publication?
yes
id
47b8254f-842e-4216-95e7-82a8698eb915
date added to LUP
2018-02-08 07:46:10
date last changed
2018-05-29 10:40:10
@article{47b8254f-842e-4216-95e7-82a8698eb915,
  abstract     = {<p>Diet-induced obesity and insulin resistance have been linked to changes in bile acid (BA) profiles, which in turn are highly dependent on the dietary composition and activity of the gut microbiota. The objective of the present study was to investigate whether the type and level of fiber had an effect on cecal BA composition when included in low- and high-fat diets. Groups of rats were fed two barley varieties, which resulted in three test diets containing three levels of β-glucans and two levels of dietary fiber. BAs were preconcentrated using hollow fiber liquid-phase microextraction and quantified by gas chromatography. The amount of the secondary BAs, lithocholic-, deoxycholic- and hyodexycholic acids was generally higher in groups fed high-fat diets compared with corresponding acids in groups fed low-fat diets (P&lt;.05). In contrast, most of the primary and the secondary BAs, ursodeoxycholic acid and β- and ω-muricholic acids, were two to five times higher (P&lt;.05) in groups fed low-fat diets than in groups fed high-fat diets. This was particularly true for groups fed the highest level of β-glucans and in some cases also the medium level. The BA profile in the gut was strongly dependent on the amount and type of dietary fiber in the diet, which may be useful in the prevention/treatment of diseases associated with changes in BA profiles.</p>},
  author       = {Ghaffarzadegan, Tannaz and Zhong, Yadong and Fåk Hållenius, Frida and Nyman, Margareta},
  issn         = {0955-2863},
  keyword      = {Barley,Bile acids,Cellulose,Dietary fiber,Gas chromatography,β-Glucans},
  language     = {eng},
  month        = {03},
  pages        = {104--110},
  publisher    = {Elsevier},
  series       = {Journal of Nutritional Biochemistry},
  title        = {Effects of barley variety, dietary fiber and β-glucan content on bile acid composition in cecum of rats fed low- and high-fat diets},
  url          = {http://dx.doi.org/},
  volume       = {53},
  year         = {2018},
}