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Pre-treatment with antibiotics and Escherichia coli to equalize the gut microbiota in conventional mice.

Linninge, Caroline LU ; Ahrné, Siv LU and Molin, Göran LU (2015) In Antonie van Leeuwenhoek 107(1). p.149-156
Abstract
The composition of the gut microbiota can vary widely between individual mice of the same batch and thereby affect the resulting outcome in experimental studies. Therefore, an efficient method is needed to equalize the gut microbiota prior to the start of critical experiments. In order to minimize variations in gut microbiota between animals and provide the animals with a Gram-negative flora exposing lipopolysaccharides in the cell-walls, C57BL/6 mice were given a mixture of ampicillin, metronidazole and clindamycin in the drinking water for 3 days and then Escherichia coli for two additional days. Treatment with antibiotics alone or with antibiotics in combination with E. coli was well tolerated by all animals. Body weight and liver... (More)
The composition of the gut microbiota can vary widely between individual mice of the same batch and thereby affect the resulting outcome in experimental studies. Therefore, an efficient method is needed to equalize the gut microbiota prior to the start of critical experiments. In order to minimize variations in gut microbiota between animals and provide the animals with a Gram-negative flora exposing lipopolysaccharides in the cell-walls, C57BL/6 mice were given a mixture of ampicillin, metronidazole and clindamycin in the drinking water for 3 days and then Escherichia coli for two additional days. Treatment with antibiotics alone or with antibiotics in combination with E. coli was well tolerated by all animals. Body weight and liver weight were not affected, although higher hepatic fat content was found in treated animals (p < 0.05). The diversity of the gut microbiota was strongly reduced in animals treated with antibiotics and antibiotics in combination with E. coli (p < 0.01), without affecting the total amount of bacteria. Cloned and sequenced 16S rRNA genes showed high presence of Enterobacteriaceae and Porphymonadaceae in the treated animals. Analysis with Principal Component Analysis gave a clear separation of the composition in microbiota between different treatment groups. The described treatment efficiently equalized the gut microbiota and provided the animals with a strong abundance of Enterobacteriaceae without changing the total load of bacteria. This is a straightforward, lenient and efficient method of pre-treatment to equalize the gut microbiota of mice as a starting procedure of animal studies. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Antonie van Leeuwenhoek
volume
107
issue
1
pages
149 - 156
publisher
Springer
external identifiers
  • pmid:25358796
  • wos:000347249500016
  • scopus:84938561068
ISSN
1572-9699
DOI
10.1007/s10482-014-0312-3
language
English
LU publication?
yes
id
b8c1d2e1-8125-472e-80e0-fcd5d0bbe2e6 (old id 4820491)
date added to LUP
2014-12-10 09:44:04
date last changed
2017-09-10 03:02:15
@article{b8c1d2e1-8125-472e-80e0-fcd5d0bbe2e6,
  abstract     = {The composition of the gut microbiota can vary widely between individual mice of the same batch and thereby affect the resulting outcome in experimental studies. Therefore, an efficient method is needed to equalize the gut microbiota prior to the start of critical experiments. In order to minimize variations in gut microbiota between animals and provide the animals with a Gram-negative flora exposing lipopolysaccharides in the cell-walls, C57BL/6 mice were given a mixture of ampicillin, metronidazole and clindamycin in the drinking water for 3 days and then Escherichia coli for two additional days. Treatment with antibiotics alone or with antibiotics in combination with E. coli was well tolerated by all animals. Body weight and liver weight were not affected, although higher hepatic fat content was found in treated animals (p &lt; 0.05). The diversity of the gut microbiota was strongly reduced in animals treated with antibiotics and antibiotics in combination with E. coli (p &lt; 0.01), without affecting the total amount of bacteria. Cloned and sequenced 16S rRNA genes showed high presence of Enterobacteriaceae and Porphymonadaceae in the treated animals. Analysis with Principal Component Analysis gave a clear separation of the composition in microbiota between different treatment groups. The described treatment efficiently equalized the gut microbiota and provided the animals with a strong abundance of Enterobacteriaceae without changing the total load of bacteria. This is a straightforward, lenient and efficient method of pre-treatment to equalize the gut microbiota of mice as a starting procedure of animal studies.},
  author       = {Linninge, Caroline and Ahrné, Siv and Molin, Göran},
  issn         = {1572-9699},
  language     = {eng},
  number       = {1},
  pages        = {149--156},
  publisher    = {Springer},
  series       = {Antonie van Leeuwenhoek},
  title        = {Pre-treatment with antibiotics and Escherichia coli to equalize the gut microbiota in conventional mice.},
  url          = {http://dx.doi.org/10.1007/s10482-014-0312-3},
  volume       = {107},
  year         = {2015},
}