The characterization of epithelial and stromal subsets of candidate stem/progenitor cells in the human adult prostate.
Ceder, Jens LU ; Jansson, Linda LU ; Ehrnström, Roy LU ; Rönnstrand, Lars LU
and Abrahamsson, Per-Anders
LU
(2008)
In European Urology
53(3).
p.524-532
- Abstract
- OBJECTIVES: Questions regarding the cell source and mechanisms in the initiation and progression of prostate cancer are today still open for debate. Indeed, our knowledge regarding prostate cell regulation, self-renewal, and cytodifferentiation is presently rather limited. In this study, we investigated these processes in the normal adult human prostate. METHODS: Dynamic expression patterns in prostate stem/progenitor cells, intermediate/transit-amplifying cells, and cell lineages were immunohistochemically identified in an in situ explant renewal model of the human normal/benign adult prostate (n=6). RESULTS: Cells with a basal phenotype proliferated significantly in explant cultures, whereas luminal cells went into apoptosis. Results... (More)
- OBJECTIVES: Questions regarding the cell source and mechanisms in the initiation and progression of prostate cancer are today still open for debate. Indeed, our knowledge regarding prostate cell regulation, self-renewal, and cytodifferentiation is presently rather limited. In this study, we investigated these processes in the normal adult human prostate. METHODS: Dynamic expression patterns in prostate stem/progenitor cells, intermediate/transit-amplifying cells, and cell lineages were immunohistochemically identified in an in situ explant renewal model of the human normal/benign adult prostate (n=6). RESULTS: Cells with a basal phenotype proliferated significantly in explant cultures, whereas luminal cells went into apoptosis. Results further show down-regulation in tissue cultures of the basal and hypothetical stem cell marker Bcl-2 in the majority of cells, except in rare putative epithelial stem cells. Investigation of established (AC133) and novel candidate prostate stem/progenitor markers, including the cell surface receptor tyrosine kinase KIT and its ligand stem cell factor (SCF), showed that these rare epithelial cells are AC133(+)/CD133(low)/Bcl-2(high)/cytokeratin(+)/vimentin(-)/KIT(low)/SCF(low). In addition, we report on a stromal population that expresses the mesenchymal marker vimentin and that is AC133(-)/CD133(high)/Bcl-2(-)/cytokeratin(-)/KIT(high)/SCF(high). CONCLUSIONS: We provide evidence for epithelial renewal in response to tissue culture and for basal and epithelial stem/progenitor cell recruitment leading to an expansion of an intermediate luminal precursor phenotype. Data further suggest that SCF regulates prostate epithelial stem/progenitor cells in an autocrine manner and that all or a subset of the identified novel stromal phenotype represents prostate stromal progenitor cells or interstitial pacemaker cells or both. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1035729
- author
- Ceder, Jens
LU
; Jansson, Linda
LU
; Ehrnström, Roy
LU
; Rönnstrand, Lars
LU
and Abrahamsson, Per-Anders
LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cell differentiation, adult stem cells, mesenchymal stem cells, prostate gland
- in
- European Urology
- volume
- 53
- issue
- 3
- pages
- 524 - 532
- publisher
- Elsevier
- external identifiers
-
- pmid:18053634
- wos:000253619800009
- scopus:38749109282
- pmid:18053634
- ISSN
- 1873-7560
- DOI
- 10.1016/j.eururo.2007.11.028
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division of urological research (013243410), Molecular Psychiatry Unit (013024100), Pathology (Malmö) (013031000), Experimental Clinical Chemistry (013016010)
- id
- 48a3685a-0f4e-4c64-9635-615dada1b365 (old id 1035729)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18053634?dopt=Abstract
- date added to LUP
- 2016-04-01 15:07:06
- date last changed
- 2022-01-28 04:32:14
@article{48a3685a-0f4e-4c64-9635-615dada1b365,
abstract = {{OBJECTIVES: Questions regarding the cell source and mechanisms in the initiation and progression of prostate cancer are today still open for debate. Indeed, our knowledge regarding prostate cell regulation, self-renewal, and cytodifferentiation is presently rather limited. In this study, we investigated these processes in the normal adult human prostate. METHODS: Dynamic expression patterns in prostate stem/progenitor cells, intermediate/transit-amplifying cells, and cell lineages were immunohistochemically identified in an in situ explant renewal model of the human normal/benign adult prostate (n=6). RESULTS: Cells with a basal phenotype proliferated significantly in explant cultures, whereas luminal cells went into apoptosis. Results further show down-regulation in tissue cultures of the basal and hypothetical stem cell marker Bcl-2 in the majority of cells, except in rare putative epithelial stem cells. Investigation of established (AC133) and novel candidate prostate stem/progenitor markers, including the cell surface receptor tyrosine kinase KIT and its ligand stem cell factor (SCF), showed that these rare epithelial cells are AC133(+)/CD133(low)/Bcl-2(high)/cytokeratin(+)/vimentin(-)/KIT(low)/SCF(low). In addition, we report on a stromal population that expresses the mesenchymal marker vimentin and that is AC133(-)/CD133(high)/Bcl-2(-)/cytokeratin(-)/KIT(high)/SCF(high). CONCLUSIONS: We provide evidence for epithelial renewal in response to tissue culture and for basal and epithelial stem/progenitor cell recruitment leading to an expansion of an intermediate luminal precursor phenotype. Data further suggest that SCF regulates prostate epithelial stem/progenitor cells in an autocrine manner and that all or a subset of the identified novel stromal phenotype represents prostate stromal progenitor cells or interstitial pacemaker cells or both.}},
author = {{Ceder, Jens and Jansson, Linda and Ehrnström, Roy and Rönnstrand, Lars and Abrahamsson, Per-Anders}},
issn = {{1873-7560}},
keywords = {{cell differentiation; adult stem cells; mesenchymal stem cells; prostate gland}},
language = {{eng}},
number = {{3}},
pages = {{524--532}},
publisher = {{Elsevier}},
series = {{European Urology}},
title = {{The characterization of epithelial and stromal subsets of candidate stem/progenitor cells in the human adult prostate.}},
url = {{http://dx.doi.org/10.1016/j.eururo.2007.11.028}},
doi = {{10.1016/j.eururo.2007.11.028}},
volume = {{53}},
year = {{2008}},
}