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A compact multifunctional microfluidic platform for exploring cellular dynamics in real-time using electrochemical detection

Zor, K.; Heiskanen, A.; Caviglia, C.; Vergani, M.; Landini, E.; Shah, F.; Carminati, M.; Martinez-Serrano, A.; Ramos Moreno, Tania LU and Kokaia, Merab LU , et al. (2014) In RSC Advances 4(109). p.63761-63771
Abstract
Downscaling of microfluidic cell culture and detection devices for electrochemical monitoring has mostly focused on miniaturization of the microfluidic chips which are often designed for specific applications and therefore lack functional flexibility. We present a compact microfluidic cell culture and electrochemical analysis platform with in-built fluid handling and detection, enabling complete cell based assays comprising on-line electrode cleaning, sterilization, surface functionalization, cell seeding, cultivation and electrochemical real-time monitoring of cellular dynamics. To demonstrate the versatility and multifunctionality of the platform, we explored amperometric monitoring of intracellular redox activity in yeast (Saccharomyces... (More)
Downscaling of microfluidic cell culture and detection devices for electrochemical monitoring has mostly focused on miniaturization of the microfluidic chips which are often designed for specific applications and therefore lack functional flexibility. We present a compact microfluidic cell culture and electrochemical analysis platform with in-built fluid handling and detection, enabling complete cell based assays comprising on-line electrode cleaning, sterilization, surface functionalization, cell seeding, cultivation and electrochemical real-time monitoring of cellular dynamics. To demonstrate the versatility and multifunctionality of the platform, we explored amperometric monitoring of intracellular redox activity in yeast (Saccharomyces cerevisiae) and detection of exocytotically released dopamine from rat pheochromocytoma cells (PC12). Electrochemical impedance spectroscopy was used in both applications for monitoring cell sedimentation and adhesion as well as proliferation in the case of PC12 cells. The influence of flow rate on the signal amplitude in the detection of redox metabolism as well as the effect of mechanical stimulation on dopamine release were demonstrated using the programmable fluid handling capability. The here presented platform is aimed at applications utilizing cell based assays, ranging from e.g. monitoring of drug effects in pharmacological studies, characterization of neural stem cell differentiation, and screening of genetically modified microorganisms to environmental monitoring. (Less)
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publication status
published
subject
in
RSC Advances
volume
4
issue
109
pages
63761 - 63771
publisher
Royal Society of Chemistry
external identifiers
  • wos:000345702900020
  • scopus:84914133160
ISSN
2046-2069
DOI
10.1039/c4ra12632g
language
English
LU publication?
yes
id
ff8d7c1c-da00-49a4-bce4-a40df63c7b05 (old id 4965775)
date added to LUP
2015-02-03 07:17:20
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2017-11-12 03:48:43
@article{ff8d7c1c-da00-49a4-bce4-a40df63c7b05,
  abstract     = {Downscaling of microfluidic cell culture and detection devices for electrochemical monitoring has mostly focused on miniaturization of the microfluidic chips which are often designed for specific applications and therefore lack functional flexibility. We present a compact microfluidic cell culture and electrochemical analysis platform with in-built fluid handling and detection, enabling complete cell based assays comprising on-line electrode cleaning, sterilization, surface functionalization, cell seeding, cultivation and electrochemical real-time monitoring of cellular dynamics. To demonstrate the versatility and multifunctionality of the platform, we explored amperometric monitoring of intracellular redox activity in yeast (Saccharomyces cerevisiae) and detection of exocytotically released dopamine from rat pheochromocytoma cells (PC12). Electrochemical impedance spectroscopy was used in both applications for monitoring cell sedimentation and adhesion as well as proliferation in the case of PC12 cells. The influence of flow rate on the signal amplitude in the detection of redox metabolism as well as the effect of mechanical stimulation on dopamine release were demonstrated using the programmable fluid handling capability. The here presented platform is aimed at applications utilizing cell based assays, ranging from e.g. monitoring of drug effects in pharmacological studies, characterization of neural stem cell differentiation, and screening of genetically modified microorganisms to environmental monitoring.},
  author       = {Zor, K. and Heiskanen, A. and Caviglia, C. and Vergani, M. and Landini, E. and Shah, F. and Carminati, M. and Martinez-Serrano, A. and Ramos Moreno, Tania and Kokaia, Merab and Benayahu, D. and Keresztes, Zs. and Papkovsky, D. and Wollenberger, U. and Svendsen, W. E. and Dimaki, M. and Ferrari, G. and Raiteri, R. and Sampietro, M. and Dufva, M. and Emneus, J.},
  issn         = {2046-2069},
  language     = {eng},
  number       = {109},
  pages        = {63761--63771},
  publisher    = {Royal Society of Chemistry},
  series       = {RSC Advances},
  title        = {A compact multifunctional microfluidic platform for exploring cellular dynamics in real-time using electrochemical detection},
  url          = {http://dx.doi.org/10.1039/c4ra12632g},
  volume       = {4},
  year         = {2014},
}