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Label-free separation of leukocyte subpopulations using high throughput multiplex acoustophoresis

Urbansky, Anke LU ; Olm, Franziska LU orcid ; Scheding, Stefan LU ; Laurell, Thomas LU and Lenshof, Andreas LU orcid (2019) In Lab on a Chip 19(8). p.1406-1416
Abstract


Multiplex separation of mixed cell samples is required in a variety of clinical and research applications. Herein, we present an acoustic microchip with multiple outlets and integrated pre-alignment channel to enable high performance and label-free separation of three different cell or particle fractions simultaneously at high sample throughput. By implementing a new cooling system for rigorous temperature control and minimal acoustic energy losses, we were able to operate the system isothermally and sort suspensions of 3, 5 and 7 μm beads with high efficiencies (>95.4%) and purities (>96.3%) at flow rates up to 500 μL min
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Multiplex separation of mixed cell samples is required in a variety of clinical and research applications. Herein, we present an acoustic microchip with multiple outlets and integrated pre-alignment channel to enable high performance and label-free separation of three different cell or particle fractions simultaneously at high sample throughput. By implementing a new cooling system for rigorous temperature control and minimal acoustic energy losses, we were able to operate the system isothermally and sort suspensions of 3, 5 and 7 μm beads with high efficiencies (>95.4%) and purities (>96.3%) at flow rates up to 500 μL min
-1
corresponding to a throughput of ∼2.5 × 10
6
beads per min. Also, human viable white blood cells were successfully fractionated into lymphocytes, monocytes and granulocytes with high purities of 96.5 ± 1.6%, 71.8 ± 10.1% and 98.8 ± 0.5%, respectively, as well as high efficiencies (96.8 ± 3.3%, 66.7 ± 3.2% and 99.0 ± 0.7%) at flow rates up to 100 μL min
-1
(∼100000 cells per min). By increasing the flow rate up to 300 μL min
-1
(∼300000 cells per min) both lymphocytes and granulocytes were still recovered with high purities (92.8 ± 1.9%, 98.2 ± 1.0%), whereas the monocyte purity decreased to 20.9 ± 10.3%. The proposed isothermal multiplex acoustophoresis platform offers efficient fractionation of complex samples in a label-free and continuous manner at thus far unreached high sample throughput rates.

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Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Lab on a Chip
volume
19
issue
8
pages
11 pages
publisher
Royal Society of Chemistry
external identifiers
  • pmid:30869100
  • scopus:85064181703
ISSN
1473-0197
DOI
10.1039/c9lc00181f
language
English
LU publication?
yes
id
4ee3cab5-1138-49fb-be40-488cf1b2db37
date added to LUP
2019-04-25 14:50:39
date last changed
2025-10-16 17:49:19
@article{4ee3cab5-1138-49fb-be40-488cf1b2db37,
  abstract     = {{<p><br>
                                                         Multiplex separation of mixed cell samples is required in a variety of clinical and research applications. Herein, we present an acoustic microchip with multiple outlets and integrated pre-alignment channel to enable high performance and label-free separation of three different cell or particle fractions simultaneously at high sample throughput. By implementing a new cooling system for rigorous temperature control and minimal acoustic energy losses, we were able to operate the system isothermally and sort suspensions of 3, 5 and 7 μm beads with high efficiencies (&gt;95.4%) and purities (&gt;96.3%) at flow rates up to 500 μL min                             <br>
                            <sup>-1</sup><br>
                                                          corresponding to a throughput of ∼2.5 × 10                             <br>
                            <sup>6</sup><br>
                                                          beads per min. Also, human viable white blood cells were successfully fractionated into lymphocytes, monocytes and granulocytes with high purities of 96.5 ± 1.6%, 71.8 ± 10.1% and 98.8 ± 0.5%, respectively, as well as high efficiencies (96.8 ± 3.3%, 66.7 ± 3.2% and 99.0 ± 0.7%) at flow rates up to 100 μL min                             <br>
                            <sup>-1</sup><br>
                                                          (∼100000 cells per min). By increasing the flow rate up to 300 μL min                             <br>
                            <sup>-1</sup><br>
                                                          (∼300000 cells per min) both lymphocytes and granulocytes were still recovered with high purities (92.8 ± 1.9%, 98.2 ± 1.0%), whereas the monocyte purity decreased to 20.9 ± 10.3%. The proposed isothermal multiplex acoustophoresis platform offers efficient fractionation of complex samples in a label-free and continuous manner at thus far unreached high sample throughput rates.                         <br>
                        </p>}},
  author       = {{Urbansky, Anke and Olm, Franziska and Scheding, Stefan and Laurell, Thomas and Lenshof, Andreas}},
  issn         = {{1473-0197}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{8}},
  pages        = {{1406--1416}},
  publisher    = {{Royal Society of Chemistry}},
  series       = {{Lab on a Chip}},
  title        = {{Label-free separation of leukocyte subpopulations using high throughput multiplex acoustophoresis}},
  url          = {{http://dx.doi.org/10.1039/c9lc00181f}},
  doi          = {{10.1039/c9lc00181f}},
  volume       = {{19}},
  year         = {{2019}},
}