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Targeted Proteomic Analyses of Nasal Lavage Fluid in Persulfate-Challenged Hairdressers with Bleaching Powder-Associated Rhinitis

Mörtstedt, Harriet LU ; Ali, Neserin LU ; Kåredal, Monica LU ; Jacobsson, Helene; Rietz, Emelie LU ; Diab, Kerstin LU ; Nielsen, Jörn LU ; Jönsson, Bo A LU and Lindh, Christian LU (2015) In Journal of Proteome Research 14(2). p.860-873
Abstract
Hairdressers have an increased risk for developing airway symptoms, for example, asthma and rhinitis. Persulfates, which are oxidizing agents in bleaching powder, are considered important causal agents for these symptoms. However, the underlying mechanisms are unclear. The aim was therefore to measure proteomic changes in nasal lavage fluid from persulfate-challenged subjects to identify proteins potentially involved in the pathogenesis of bleaching powder-associated rhinitis or candidate effect biomarkers for persulfate. Also, oxidized peptides were measured to evaluate their usefulness as biomarkers for persulfate exposure or effect, for example, oxidative stress. Samples from hairdressers with and without bleaching powder-associated... (More)
Hairdressers have an increased risk for developing airway symptoms, for example, asthma and rhinitis. Persulfates, which are oxidizing agents in bleaching powder, are considered important causal agents for these symptoms. However, the underlying mechanisms are unclear. The aim was therefore to measure proteomic changes in nasal lavage fluid from persulfate-challenged subjects to identify proteins potentially involved in the pathogenesis of bleaching powder-associated rhinitis or candidate effect biomarkers for persulfate. Also, oxidized peptides were measured to evaluate their usefulness as biomarkers for persulfate exposure or effect, for example, oxidative stress. Samples from hairdressers with and without bleaching powder-associated rhinitis were analyzed with liquid chromatography tandem mass spectrometry using selected reaction monitoring to target 246 proteins and five oxidized peptides. Pathway analysis was applied to obtain a functional overview of the proteins. Several proteins involved in biologically meaningful pathways, functions, or disorders, for example, inflammatory responses, oxidative stress, epithelium integrity, and dermatological disorders, changed after the persulfate challenge. A list with nine proteins that appeared to be affected by the persulfate challenge and should be followed up was defined. An albumin peptide containing oxidized tryptophan increased 2 h and 5 h after the challenge but not after 20 min, which indicates that such peptides may be useful as oxidative stress biomarkers. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
nasal lavage fluid, selected reaction monitoring, persulfate, hairdresser, proteomics, oxidation, mass spectrometry, pathway analysis, airways, LC-MS/MS
in
Journal of Proteome Research
volume
14
issue
2
pages
860 - 873
publisher
The American Chemical Society
external identifiers
  • wos:000349276400026
  • scopus:84922665433
ISSN
1535-3893
DOI
10.1021/pr5009306
language
English
LU publication?
yes
id
2fc4478e-5c82-418e-8d92-e0de7ccc8097 (old id 5160280)
date added to LUP
2015-04-01 07:39:32
date last changed
2017-04-23 03:08:44
@article{2fc4478e-5c82-418e-8d92-e0de7ccc8097,
  abstract     = {Hairdressers have an increased risk for developing airway symptoms, for example, asthma and rhinitis. Persulfates, which are oxidizing agents in bleaching powder, are considered important causal agents for these symptoms. However, the underlying mechanisms are unclear. The aim was therefore to measure proteomic changes in nasal lavage fluid from persulfate-challenged subjects to identify proteins potentially involved in the pathogenesis of bleaching powder-associated rhinitis or candidate effect biomarkers for persulfate. Also, oxidized peptides were measured to evaluate their usefulness as biomarkers for persulfate exposure or effect, for example, oxidative stress. Samples from hairdressers with and without bleaching powder-associated rhinitis were analyzed with liquid chromatography tandem mass spectrometry using selected reaction monitoring to target 246 proteins and five oxidized peptides. Pathway analysis was applied to obtain a functional overview of the proteins. Several proteins involved in biologically meaningful pathways, functions, or disorders, for example, inflammatory responses, oxidative stress, epithelium integrity, and dermatological disorders, changed after the persulfate challenge. A list with nine proteins that appeared to be affected by the persulfate challenge and should be followed up was defined. An albumin peptide containing oxidized tryptophan increased 2 h and 5 h after the challenge but not after 20 min, which indicates that such peptides may be useful as oxidative stress biomarkers.},
  author       = {Mörtstedt, Harriet and Ali, Neserin and Kåredal, Monica and Jacobsson, Helene and Rietz, Emelie and Diab, Kerstin and Nielsen, Jörn and Jönsson, Bo A and Lindh, Christian},
  issn         = {1535-3893},
  keyword      = {nasal lavage fluid,selected reaction monitoring,persulfate,hairdresser,proteomics,oxidation,mass spectrometry,pathway analysis,airways,LC-MS/MS},
  language     = {eng},
  number       = {2},
  pages        = {860--873},
  publisher    = {The American Chemical Society},
  series       = {Journal of Proteome Research},
  title        = {Targeted Proteomic Analyses of Nasal Lavage Fluid in Persulfate-Challenged Hairdressers with Bleaching Powder-Associated Rhinitis},
  url          = {http://dx.doi.org/10.1021/pr5009306},
  volume       = {14},
  year         = {2015},
}