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Fluorescence and absorption assessment of a lipid mTHPC formulation following topical application in a non-melanotic skin tumor model.

Johansson, Ann LU ; Svensson, Jenny LU ; Bendsöe, Niels LU ; Svanberg, Katarina LU ; Alexandratou, Eleni; Kyriazi, Maria; Yova, Dido; Grafe, Susanna; Trebst, Tilmann and Andersson-Engels, Stefan LU (2007) In Journal of Biomedical Optics 12(3). p.034026-034026
Abstract
Although the benefits of topical sensitizer administration have been confirmed for photodynamic therapy (PDT) ALA-induced, protoporphyrin IX is the only sensitizer clinically used with this administration route. Unfortunately, ALA-PDT results in poor treatment response for thicker lesions. Here, selectivity and depth distribution of the highly potent sensitizer meso-tetra(hydroxyphenyl)chlorin (mTHPC), supplied in a novel liposome formulation was investigated following topical administration for 4 and 6 h in a murine skin tumor model. Extraction data indicated an average [standard deviation (SD)] mTHPC concentration within lesions of 6.0(+/- 3.1) ng/mg tissue with no significant difference (p<0.05) between 4- and 6-h application times... (More)
Although the benefits of topical sensitizer administration have been confirmed for photodynamic therapy (PDT) ALA-induced, protoporphyrin IX is the only sensitizer clinically used with this administration route. Unfortunately, ALA-PDT results in poor treatment response for thicker lesions. Here, selectivity and depth distribution of the highly potent sensitizer meso-tetra(hydroxyphenyl)chlorin (mTHPC), supplied in a novel liposome formulation was investigated following topical administration for 4 and 6 h in a murine skin tumor model. Extraction data indicated an average [standard deviation (SD)] mTHPC concentration within lesions of 6.0(+/- 3.1) ng/mg tissue with no significant difference (p<0.05) between 4- and 6-h application times and undetectable levels of generalized photosensitivity. Absorption spectroscopy and chemical extraction both indicated a significant selectivity between lesion and normal surrounding skin at 4 and 6 h, whereas the more sensitive fluorescence imaging setup revealed significant selectivity only for the 4-h application time. Absorption data showed a significant correlation with extraction, whereas the results from the fluorescence imaging setup did not correlate with the other methods. Our results indicate that this sensitizer formulation and administration path could be interesting for topical mTHPC-PDT, decreasing the effects of extended skin photosensitivity associated with systemic mTHPC administration. (C) 2007 Society of Photo-Optical instrumentation Engineers. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
mTHPC, photodynamic therapy, absorption spectroscopy, pharmacokinetics, fluorescence imaging
in
Journal of Biomedical Optics
volume
12
issue
3
pages
034026 - 034026
publisher
Published by SPIE--the International Society for Optical Engineering in cooperation with International Biomedical Optics Society
external identifiers
  • wos:000248504500030
  • scopus:34547904584
ISSN
1083-3668
DOI
10.1117/1.2743080
language
English
LU publication?
yes
id
3fcad7bc-52e4-44a8-9c48-79882014645b (old id 540994)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17614734&dopt=Abstract
date added to LUP
2008-10-28 10:44:43
date last changed
2017-01-01 05:00:34
@article{3fcad7bc-52e4-44a8-9c48-79882014645b,
  abstract     = {Although the benefits of topical sensitizer administration have been confirmed for photodynamic therapy (PDT) ALA-induced, protoporphyrin IX is the only sensitizer clinically used with this administration route. Unfortunately, ALA-PDT results in poor treatment response for thicker lesions. Here, selectivity and depth distribution of the highly potent sensitizer meso-tetra(hydroxyphenyl)chlorin (mTHPC), supplied in a novel liposome formulation was investigated following topical administration for 4 and 6 h in a murine skin tumor model. Extraction data indicated an average [standard deviation (SD)] mTHPC concentration within lesions of 6.0(+/- 3.1) ng/mg tissue with no significant difference (p&lt;0.05) between 4- and 6-h application times and undetectable levels of generalized photosensitivity. Absorption spectroscopy and chemical extraction both indicated a significant selectivity between lesion and normal surrounding skin at 4 and 6 h, whereas the more sensitive fluorescence imaging setup revealed significant selectivity only for the 4-h application time. Absorption data showed a significant correlation with extraction, whereas the results from the fluorescence imaging setup did not correlate with the other methods. Our results indicate that this sensitizer formulation and administration path could be interesting for topical mTHPC-PDT, decreasing the effects of extended skin photosensitivity associated with systemic mTHPC administration. (C) 2007 Society of Photo-Optical instrumentation Engineers.},
  author       = {Johansson, Ann and Svensson, Jenny and Bendsöe, Niels and Svanberg, Katarina and Alexandratou, Eleni and Kyriazi, Maria and Yova, Dido and Grafe, Susanna and Trebst, Tilmann and Andersson-Engels, Stefan},
  issn         = {1083-3668},
  keyword      = {mTHPC,photodynamic therapy,absorption spectroscopy,pharmacokinetics,fluorescence imaging},
  language     = {eng},
  number       = {3},
  pages        = {034026--034026},
  publisher    = {Published by SPIE--the International Society for Optical Engineering in cooperation with International Biomedical Optics Society},
  series       = {Journal of Biomedical Optics},
  title        = {Fluorescence and absorption assessment of a lipid mTHPC formulation following topical application in a non-melanotic skin tumor model.},
  url          = {http://dx.doi.org/10.1117/1.2743080},
  volume       = {12},
  year         = {2007},
}