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Collagen VI Is Upregulated in COPD and Serves Both as an Adhesive Target and a Bactericidal Barrier for Moraxella catarrhalis.

M Abdillahi, Suado LU ; Bober, Marta LU ; Nordin, Sara LU ; Hallgren, Oskar LU ; Baumgarten, Maria LU ; Erjefält, Jonas LU ; Westergren-Thorsson, Gunilla LU ; Bjermer, Leif LU ; Riesbeck, Kristian LU and Egesten, Arne LU , et al. (2015) In Journal of Innate Immunity 7(5). p.506-517
Abstract
Moraxella catarrhalis is a Gram-negative human mucosal commensal and pathogen. It is a common cause of exacerbation in chronic obstructive pulmonary disease (COPD). During the process of infection, host colonization correlates with recognition of host molecular patterns. Importantly, in COPD patients with compromised epithelial integrity the underlying extracellular matrix is exposed and provides potential adhesive targets. Collagen VI is a ubiquitous fibrillar component in the airway mucosa and has been attributed both adhesive and killing properties against Gram-positive bacteria. However, less is known regarding Gram-negative microorganisms. Therefore, in the present study, the interaction of M. catarrhalis with collagen VI was... (More)
Moraxella catarrhalis is a Gram-negative human mucosal commensal and pathogen. It is a common cause of exacerbation in chronic obstructive pulmonary disease (COPD). During the process of infection, host colonization correlates with recognition of host molecular patterns. Importantly, in COPD patients with compromised epithelial integrity the underlying extracellular matrix is exposed and provides potential adhesive targets. Collagen VI is a ubiquitous fibrillar component in the airway mucosa and has been attributed both adhesive and killing properties against Gram-positive bacteria. However, less is known regarding Gram-negative microorganisms. Therefore, in the present study, the interaction of M. catarrhalis with collagen VI was characterized. We found that collagen VI is upregulated in the airways of COPD patients and exposed upon epithelial desquamation. Ex vivo, we inoculated airway biopsies and fibroblasts from COPD patients with M. catarrhalis. The bacteria specifically adhered to collagen VI-containing matrix fibrils. In vitro, purified collagen VI microfibrils bound to bacterial surface structures. The primary adhesion target was mapped to the collagen VI α2-chain. Upon exposure to collagen VI, bacteria were killed by membrane destabilization in physiological conditions. These previously unknown properties of collagen VI provide novel insights into the extracellular matrix innate immunity by quickly entrapping and killing pathogen intruders. © 2015 S. Karger AG, Basel. (Less)
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Journal of Innate Immunity
volume
7
issue
5
pages
506 - 517
publisher
Karger
external identifiers
  • pmid:25925694
  • wos:000360469300008
  • scopus:84941994934
ISSN
1662-811X
DOI
10.1159/000381213
language
English
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yes
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a6862087-8bdd-427f-9a9b-0d4bdf235b46 (old id 5461779)
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http://www.ncbi.nlm.nih.gov/pubmed/25925694?dopt=Abstract
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2015-06-04 11:52:29
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2017-01-29 03:00:54
@article{a6862087-8bdd-427f-9a9b-0d4bdf235b46,
  abstract     = {Moraxella catarrhalis is a Gram-negative human mucosal commensal and pathogen. It is a common cause of exacerbation in chronic obstructive pulmonary disease (COPD). During the process of infection, host colonization correlates with recognition of host molecular patterns. Importantly, in COPD patients with compromised epithelial integrity the underlying extracellular matrix is exposed and provides potential adhesive targets. Collagen VI is a ubiquitous fibrillar component in the airway mucosa and has been attributed both adhesive and killing properties against Gram-positive bacteria. However, less is known regarding Gram-negative microorganisms. Therefore, in the present study, the interaction of M. catarrhalis with collagen VI was characterized. We found that collagen VI is upregulated in the airways of COPD patients and exposed upon epithelial desquamation. Ex vivo, we inoculated airway biopsies and fibroblasts from COPD patients with M. catarrhalis. The bacteria specifically adhered to collagen VI-containing matrix fibrils. In vitro, purified collagen VI microfibrils bound to bacterial surface structures. The primary adhesion target was mapped to the collagen VI α2-chain. Upon exposure to collagen VI, bacteria were killed by membrane destabilization in physiological conditions. These previously unknown properties of collagen VI provide novel insights into the extracellular matrix innate immunity by quickly entrapping and killing pathogen intruders. © 2015 S. Karger AG, Basel.},
  author       = {M Abdillahi, Suado and Bober, Marta and Nordin, Sara and Hallgren, Oskar and Baumgarten, Maria and Erjefält, Jonas and Westergren-Thorsson, Gunilla and Bjermer, Leif and Riesbeck, Kristian and Egesten, Arne and Mörgelin, Matthias},
  issn         = {1662-811X},
  language     = {eng},
  number       = {5},
  pages        = {506--517},
  publisher    = {Karger},
  series       = {Journal of Innate Immunity},
  title        = {Collagen VI Is Upregulated in COPD and Serves Both as an Adhesive Target and a Bactericidal Barrier for Moraxella catarrhalis.},
  url          = {http://dx.doi.org/10.1159/000381213},
  volume       = {7},
  year         = {2015},
}