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Rapid determination of short-chain fatty acids in colonic contents and faeces of humans and rats by acidified water-extraction and direct-injection gas chromatography

Zhao, Guohua LU ; Nyman, Margareta LU and Jönsson, Jan Åke LU (2006) In Biomedical Chromatography 20(8). p.674-682
Abstract
Short-chain fatty acids (SCFAs) have attracted much attention recently because of their positive physiological effects. In this work, a rapid and reliable gas chromatographic method for determination of eight SCFAs, in colonic and faecal samples from rats and humans has been developed and validated. The methodology involves extraction of the SCFAs in water before a direct injection procedure on a FFAP capillary column. A stock standard solution containing acetic acid, propionic acid, n-butyric acid, i-butyric acid, n-valeric acid, i-valeric acid, n-caproic acid and n-heptanoic acid was prepared and used. A high linearity (r(2) > 0.9990), low quantification limit (2.38-30.14 mu M) and high recovery for most acids were obtained.... (More)
Short-chain fatty acids (SCFAs) have attracted much attention recently because of their positive physiological effects. In this work, a rapid and reliable gas chromatographic method for determination of eight SCFAs, in colonic and faecal samples from rats and humans has been developed and validated. The methodology involves extraction of the SCFAs in water before a direct injection procedure on a FFAP capillary column. A stock standard solution containing acetic acid, propionic acid, n-butyric acid, i-butyric acid, n-valeric acid, i-valeric acid, n-caproic acid and n-heptanoic acid was prepared and used. A high linearity (r(2) > 0.9990), low quantification limit (2.38-30.14 mu M) and high recovery for most acids were obtained. Acidification of faecal samples was found to be crucial for quantitative determination of the SCFAs, and adjustment of pH to 2-3 was regarded as necessary. Glass wool inserted in the glass liner of the injection port proved effective in preventing the contamination of the column by non-volatiles, and 12% formic acid reduced the ghost peak that appeared gradually after several injections. After validation, the methodology was applied on two faecal samples from rats fed diets containing different amount of dietary fibre and one faecal sample from human fed a normal diet to test the accuracy of the developed method. Copyright (c) 2005 John Wiley & Sons, Ltd. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
water extraction, direct injection, short-chain fatty acids (SCFAs), gas chromatography, faeces, colonic contents
in
Biomedical Chromatography
volume
20
issue
8
pages
674 - 682
publisher
John Wiley & Sons
external identifiers
  • wos:000239541300002
  • pmid:16206138
  • scopus:33746936566
ISSN
0269-3879
DOI
10.1002/bmc.580
language
English
LU publication?
yes
id
563d5fe3-d911-4011-910f-61952db97660 (old id 399227)
date added to LUP
2007-10-21 08:53:40
date last changed
2018-07-08 03:59:51
@article{563d5fe3-d911-4011-910f-61952db97660,
  abstract     = {Short-chain fatty acids (SCFAs) have attracted much attention recently because of their positive physiological effects. In this work, a rapid and reliable gas chromatographic method for determination of eight SCFAs, in colonic and faecal samples from rats and humans has been developed and validated. The methodology involves extraction of the SCFAs in water before a direct injection procedure on a FFAP capillary column. A stock standard solution containing acetic acid, propionic acid, n-butyric acid, i-butyric acid, n-valeric acid, i-valeric acid, n-caproic acid and n-heptanoic acid was prepared and used. A high linearity (r(2) > 0.9990), low quantification limit (2.38-30.14 mu M) and high recovery for most acids were obtained. Acidification of faecal samples was found to be crucial for quantitative determination of the SCFAs, and adjustment of pH to 2-3 was regarded as necessary. Glass wool inserted in the glass liner of the injection port proved effective in preventing the contamination of the column by non-volatiles, and 12% formic acid reduced the ghost peak that appeared gradually after several injections. After validation, the methodology was applied on two faecal samples from rats fed diets containing different amount of dietary fibre and one faecal sample from human fed a normal diet to test the accuracy of the developed method. Copyright (c) 2005 John Wiley & Sons, Ltd.},
  author       = {Zhao, Guohua and Nyman, Margareta and Jönsson, Jan Åke},
  issn         = {0269-3879},
  keyword      = {water extraction,direct injection,short-chain fatty acids (SCFAs),gas chromatography,faeces,colonic contents},
  language     = {eng},
  number       = {8},
  pages        = {674--682},
  publisher    = {John Wiley & Sons},
  series       = {Biomedical Chromatography},
  title        = {Rapid determination of short-chain fatty acids in colonic contents and faeces of humans and rats by acidified water-extraction and direct-injection gas chromatography},
  url          = {http://dx.doi.org/10.1002/bmc.580},
  volume       = {20},
  year         = {2006},
}