Optimisation of initial cell concentration enhances freeze-drying tolerance of Pseudomonas chlororaphis.
(2003) In Cryobiology 47(1). p.21-29- Abstract
- The freeze-drying tolerance of Pseudomonas chlororaphis, an antifungal bacterium used as biocontrol agent was investigated. P. chlororaphis is freeze-drying sensitive and the viability drops more than 3 log units in the absence of protective freeze-drying medium. Of the freeze-drying media tested, lactose, sucrose, trehalose, glutamate, sucrose with glutamate, skimmed milk, and skimmed milk with trehalose, skimmed milk gave the lowest survival (0.6 ± 0.2%) and sucrose the highest (6.4 ± 1.2%). Cellular accumulation of sucrose from the freeze-drying medium and the protective effect of sucrose were dependent on sucrose concentration. The effect of initial cell concentration, from 1 × 107 to 5 × 1010 CFU/ml, on survival after freeze-drying... (More)
- The freeze-drying tolerance of Pseudomonas chlororaphis, an antifungal bacterium used as biocontrol agent was investigated. P. chlororaphis is freeze-drying sensitive and the viability drops more than 3 log units in the absence of protective freeze-drying medium. Of the freeze-drying media tested, lactose, sucrose, trehalose, glutamate, sucrose with glutamate, skimmed milk, and skimmed milk with trehalose, skimmed milk gave the lowest survival (0.6 ± 0.2%) and sucrose the highest (6.4 ± 1.2%). Cellular accumulation of sucrose from the freeze-drying medium and the protective effect of sucrose were dependent on sucrose concentration. The effect of initial cell concentration, from 1 × 107 to 5 × 1010 CFU/ml, on survival after freeze-drying was studied for carbon starved cells with sucrose as freeze-drying medium. The highest freeze-drying survival values, 15–25%, were obtained for initial cell concentrations between 1 × 109 and 1 × 1010 CFU/ml. For cell concentrations outside this window more than 10 times lower survival values were observed. P. chlororaphis was cultivated to induce stress response that could confer protection against freeze-drying inactivation. Carbon starvation and, to a lesser extent, heat treatment enhanced freeze-drying tolerance. By combining optimal cell concentration, optimal sucrose concentration and carbon starvation the survival after freeze-drying was 26 ± 6%. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/132828
- author
- Palmfeldt, Johan LU ; Rådström, Peter LU and Hahn-Hägerdal, Bärbel LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Freeze-drying, Gram-negative bacteria, Sucrose, Cryoprotectant, Pseudomonas chlororaphis, Stress, Storage, Lyophilization
- in
- Cryobiology
- volume
- 47
- issue
- 1
- pages
- 21 - 29
- publisher
- Elsevier
- external identifiers
-
- wos:000220794400003
- pmid:12963409
- scopus:0042831405
- ISSN
- 0011-2240
- DOI
- 10.1016/S0011-2240(03)00065-8
- language
- English
- LU publication?
- yes
- id
- 5670d2e1-9c21-4004-9454-4dd7ecdf5e8e (old id 132828)
- date added to LUP
- 2016-04-01 12:28:13
- date last changed
- 2022-01-27 05:32:06
@article{5670d2e1-9c21-4004-9454-4dd7ecdf5e8e,
abstract = {{The freeze-drying tolerance of Pseudomonas chlororaphis, an antifungal bacterium used as biocontrol agent was investigated. P. chlororaphis is freeze-drying sensitive and the viability drops more than 3 log units in the absence of protective freeze-drying medium. Of the freeze-drying media tested, lactose, sucrose, trehalose, glutamate, sucrose with glutamate, skimmed milk, and skimmed milk with trehalose, skimmed milk gave the lowest survival (0.6 ± 0.2%) and sucrose the highest (6.4 ± 1.2%). Cellular accumulation of sucrose from the freeze-drying medium and the protective effect of sucrose were dependent on sucrose concentration. The effect of initial cell concentration, from 1 × 107 to 5 × 1010 CFU/ml, on survival after freeze-drying was studied for carbon starved cells with sucrose as freeze-drying medium. The highest freeze-drying survival values, 15–25%, were obtained for initial cell concentrations between 1 × 109 and 1 × 1010 CFU/ml. For cell concentrations outside this window more than 10 times lower survival values were observed. P. chlororaphis was cultivated to induce stress response that could confer protection against freeze-drying inactivation. Carbon starvation and, to a lesser extent, heat treatment enhanced freeze-drying tolerance. By combining optimal cell concentration, optimal sucrose concentration and carbon starvation the survival after freeze-drying was 26 ± 6%.}},
author = {{Palmfeldt, Johan and Rådström, Peter and Hahn-Hägerdal, Bärbel}},
issn = {{0011-2240}},
keywords = {{Freeze-drying; Gram-negative bacteria; Sucrose; Cryoprotectant; Pseudomonas chlororaphis; Stress; Storage; Lyophilization}},
language = {{eng}},
number = {{1}},
pages = {{21--29}},
publisher = {{Elsevier}},
series = {{Cryobiology}},
title = {{Optimisation of initial cell concentration enhances freeze-drying tolerance of Pseudomonas chlororaphis.}},
url = {{http://dx.doi.org/10.1016/S0011-2240(03)00065-8}},
doi = {{10.1016/S0011-2240(03)00065-8}},
volume = {{47}},
year = {{2003}},
}