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Fluorescence monitoring of a topically applied liposomal temoporfin formulation and photodynamic therapy of nonpigmented skin malignancies.

Bendsoe, Niels; Persson, Linda LU ; Johansson, Ann LU ; Axelsson, Johan LU ; Svensson, Jenny LU ; Grafe, Susanna; Trebst, Tilmann; Andersson-Engels, Stefan LU ; Svanberg, Sune LU and Svanberg, Katarina LU (2007) In Journal of Environmental Pathology and Toxicology 26(2). p.117-126
Abstract
Meso-tetra(hydroxyphenyl)chlorin (mTHPC) (INN: Temoporfin) is a potent photodynamically active substance in clinical use today. Usually, the substance is given systemically and a known drawback with this administration route is a prolonged skin light sensitization. For the first time to our knowledge, a liposomal Temoporfin gel formulation for topical application was studied in connection with photodynamic therapy (PDT) of nonpigmented skin malignancies in humans. Intervals of 4 hr between drug administration and light irradiation were used. Sensitizer distribution within tumor and surrounding normal skin was investigated by means of point monitoring and imaging fluorescence spectroscopy before, during, and after PDT, showing high tumor... (More)
Meso-tetra(hydroxyphenyl)chlorin (mTHPC) (INN: Temoporfin) is a potent photodynamically active substance in clinical use today. Usually, the substance is given systemically and a known drawback with this administration route is a prolonged skin light sensitization. For the first time to our knowledge, a liposomal Temoporfin gel formulation for topical application was studied in connection with photodynamic therapy (PDT) of nonpigmented skin malignancies in humans. Intervals of 4 hr between drug administration and light irradiation were used. Sensitizer distribution within tumor and surrounding normal skin was investigated by means of point monitoring and imaging fluorescence spectroscopy before, during, and after PDT, showing high tumor selectivity. Furthermore, the bleaching of Temoporfin was studied during the PDT procedure by monitoring the fluorescence following excitation by using a therapeutic light. A 30−35% light-induced photometabolization was shown. No pain occurred during or after treatment. It was also observed that the treated area did not show any swollen tissue or reddening, as is often seen in PDT using topical δ-aminolevulinic acid. On controlling the patients one week after treatment, healing progress was observed in several patients and no complications were registered. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
in
Journal of Environmental Pathology and Toxicology
volume
26
issue
2
pages
117 - 126
publisher
Begell House
external identifiers
  • wos:000248597200006
  • scopus:34548129673
ISSN
2162-6537
language
English
LU publication?
yes
id
112e5100-71b8-4152-8879-5fb991593660 (old id 606601)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17725537&dopt=Abstract
date added to LUP
2007-11-21 14:44:12
date last changed
2017-09-03 04:46:50
@article{112e5100-71b8-4152-8879-5fb991593660,
  abstract     = {Meso-tetra(hydroxyphenyl)chlorin (mTHPC) (INN: Temoporfin) is a potent photodynamically active substance in clinical use today. Usually, the substance is given systemically and a known drawback with this administration route is a prolonged skin light sensitization. For the first time to our knowledge, a liposomal Temoporfin gel formulation for topical application was studied in connection with photodynamic therapy (PDT) of nonpigmented skin malignancies in humans. Intervals of 4 hr between drug administration and light irradiation were used. Sensitizer distribution within tumor and surrounding normal skin was investigated by means of point monitoring and imaging fluorescence spectroscopy before, during, and after PDT, showing high tumor selectivity. Furthermore, the bleaching of Temoporfin was studied during the PDT procedure by monitoring the fluorescence following excitation by using a therapeutic light. A 30−35% light-induced photometabolization was shown. No pain occurred during or after treatment. It was also observed that the treated area did not show any swollen tissue or reddening, as is often seen in PDT using topical δ-aminolevulinic acid. On controlling the patients one week after treatment, healing progress was observed in several patients and no complications were registered.},
  author       = {Bendsoe, Niels and Persson, Linda and Johansson, Ann and Axelsson, Johan and Svensson, Jenny and Grafe, Susanna and Trebst, Tilmann and Andersson-Engels, Stefan and Svanberg, Sune and Svanberg, Katarina},
  issn         = {2162-6537},
  language     = {eng},
  number       = {2},
  pages        = {117--126},
  publisher    = {Begell House},
  series       = {Journal of Environmental Pathology and Toxicology},
  title        = {Fluorescence monitoring of a topically applied liposomal temoporfin formulation and photodynamic therapy of nonpigmented skin malignancies.},
  volume       = {26},
  year         = {2007},
}