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Mass spectrometric monitoring of in vitro formed ad ducts between hexahydrophthalic anhydride (HHPA) and cysteine and lysine

Kåredal, Monica LU orcid ; Ahlfors, Stefan LU ; Lindh, Christian LU orcid and Jönsson, Bo A LU (2002) Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics p.325-326
Abstract
The in vitro formed adducts between hexahydrophthalic anhydride (HHPA) and cysteine and lysine were monitored by mass spectrometry. Freshly synthesized N-acetyl-S-hexahydrophthaloyl-L-cysteine was dissolved in dry acetonitrile and added to a solution of N-acetyl-L-lysine in 0.1 M sodium phosphate buffer (pH 7.4) an incubated at 37°C. The reactions were monitored by LC/MS on a triple quadrupole mass spectrometer with electrospray ionization coupled to a HPLC system from Perkin Elmer. The results show that there was a rapid rearrangement of HHPA from most of the cysteines to the lysines, and that HHPA-adducts of cysteine will be transferred to lysine when encountered.
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Electrospray ionization (ESI), Multiple reaction monitoring (MRM), Hexahydrophthalic anhydride (HHPA), Ion spray voltage
host publication
Proceedings 50th ASMS Conference on Mass Spectrmetry and Allied Topics
pages
325 - 326
publisher
American Society for Mass Spectrometry, Santa Fe, NM, 87505, United States
conference name
Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
conference location
Orlando, FL, United States
conference dates
2002-06-02 - 2002-06-06
external identifiers
  • scopus:2442710423
language
English
LU publication?
yes
id
d920d204-3f2e-434a-bcf0-4fbed75f052e (old id 611306)
date added to LUP
2016-04-04 11:02:35
date last changed
2022-02-13 20:44:10
@inproceedings{d920d204-3f2e-434a-bcf0-4fbed75f052e,
  abstract     = {{The in vitro formed adducts between hexahydrophthalic anhydride (HHPA) and cysteine and lysine were monitored by mass spectrometry. Freshly synthesized N-acetyl-S-hexahydrophthaloyl-L-cysteine was dissolved in dry acetonitrile and added to a solution of N-acetyl-L-lysine in 0.1 M sodium phosphate buffer (pH 7.4) an incubated at 37°C. The reactions were monitored by LC/MS on a triple quadrupole mass spectrometer with electrospray ionization coupled to a HPLC system from Perkin Elmer. The results show that there was a rapid rearrangement of HHPA from most of the cysteines to the lysines, and that HHPA-adducts of cysteine will be transferred to lysine when encountered.}},
  author       = {{Kåredal, Monica and Ahlfors, Stefan and Lindh, Christian and Jönsson, Bo A}},
  booktitle    = {{Proceedings 50th ASMS Conference on Mass Spectrmetry and Allied Topics}},
  keywords     = {{Electrospray ionization (ESI); Multiple reaction monitoring (MRM); Hexahydrophthalic anhydride (HHPA); Ion spray voltage}},
  language     = {{eng}},
  pages        = {{325--326}},
  publisher    = {{American Society for Mass Spectrometry, Santa Fe, NM, 87505, United States}},
  title        = {{Mass spectrometric monitoring of in vitro formed ad ducts between hexahydrophthalic anhydride (HHPA) and cysteine and lysine}},
  year         = {{2002}},
}