mTHPC pharmacokinetics following topical administration
(2006) Optical Diagnostics and Sensing VI 6094. p.940-940- Abstract
- Measurements of concentration of sensitizers for photodynamic therapy can provide important information in the dosimetry planning and can also give input to the optimal time for treatment. There has been skepticism towards fluorescence techniques for this purpose, as the signal depends on the fluorescence yield and optical properties of the tissue. Absorption based techniques, lack on the other hand, often the sensitivity required for many sensitizers with relative weak absorption in a wavelength region where hemoglobin absorption is dominant. A direct comparison between absorption and fluorescence techniques for measuring mTHPC concentration after topical application on hairless SKH-1 mice bearing skin carcinomas has been performed. 20... (More)
- Measurements of concentration of sensitizers for photodynamic therapy can provide important information in the dosimetry planning and can also give input to the optimal time for treatment. There has been skepticism towards fluorescence techniques for this purpose, as the signal depends on the fluorescence yield and optical properties of the tissue. Absorption based techniques, lack on the other hand, often the sensitivity required for many sensitizers with relative weak absorption in a wavelength region where hemoglobin absorption is dominant. A direct comparison between absorption and fluorescence techniques for measuring mTHPC concentration after topical application on hairless SKH-1 mice bearing skin carcinomas has been performed. 20 μl/cm<sup>2</sup> of m-THPC thermogel (0.5 mg m-THPC/ml) were applied on normal and tumor area and the concentration of mTHPC was measured at 4 and 6 hours after drug application by two methods: 1. A fluorescence imaging system capturing images at two wavelengths (500 and 650 nm) following 405 nm excitation. Signals from different regions of interest were averaged and the intensity ratio at 650 to 500 was calculated. 2. A diffuse reflectance spectroscopy system with a fiber separation of 2 mm, providing the absorbance at 652 nm. Both systems provided consistent results related to the photosensitizer concentration. The methods show a remarkable difference in the concentration of photosensitizer in normal skin and tumor. No significant difference in mTHPC concentration in tumor could be observed between the 4 and 6h groups after drug application. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/617213
- author
- organization
- publishing date
- 2006
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- MTHPC, Skin carcinomas, Fluorescence imaging, Fluorescence spectroscopy
- host publication
- Optical Diagnostics and Sensing VI
- volume
- 6094
- pages
- 940 - 940
- publisher
- International Society for Optical Engineering
- conference name
- Optical Diagnostics and Sensing VI
- conference location
- San Jose, CA, United States
- conference dates
- 2006-01-24 - 2006-01-25
- external identifiers
-
- wos:000237703800008
- scopus:33745298759
- ISSN
- 1996-756X
- 0277-786X
- DOI
- 10.1117/12.647602
- language
- English
- LU publication?
- yes
- id
- 8053a720-5447-4bfb-a9f0-0cc2cf3cc9f4 (old id 617213)
- date added to LUP
- 2016-04-01 12:07:32
- date last changed
- 2025-01-02 06:38:42
@inproceedings{8053a720-5447-4bfb-a9f0-0cc2cf3cc9f4,
abstract = {{Measurements of concentration of sensitizers for photodynamic therapy can provide important information in the dosimetry planning and can also give input to the optimal time for treatment. There has been skepticism towards fluorescence techniques for this purpose, as the signal depends on the fluorescence yield and optical properties of the tissue. Absorption based techniques, lack on the other hand, often the sensitivity required for many sensitizers with relative weak absorption in a wavelength region where hemoglobin absorption is dominant. A direct comparison between absorption and fluorescence techniques for measuring mTHPC concentration after topical application on hairless SKH-1 mice bearing skin carcinomas has been performed. 20 μl/cm<sup>2</sup> of m-THPC thermogel (0.5 mg m-THPC/ml) were applied on normal and tumor area and the concentration of mTHPC was measured at 4 and 6 hours after drug application by two methods: 1. A fluorescence imaging system capturing images at two wavelengths (500 and 650 nm) following 405 nm excitation. Signals from different regions of interest were averaged and the intensity ratio at 650 to 500 was calculated. 2. A diffuse reflectance spectroscopy system with a fiber separation of 2 mm, providing the absorbance at 652 nm. Both systems provided consistent results related to the photosensitizer concentration. The methods show a remarkable difference in the concentration of photosensitizer in normal skin and tumor. No significant difference in mTHPC concentration in tumor could be observed between the 4 and 6h groups after drug application.}},
author = {{Johansson, Ann and Svensson, Jenny and Andersson-Engels, Stefan and Bendsöe, Niels and Svanberg, Katarina and Bigio, Irving and Alexandratou, Eleni and Kyriazi, Maria and Yova, Dido and Grafe, Susanna and Trebst, Tilmann}},
booktitle = {{Optical Diagnostics and Sensing VI}},
issn = {{1996-756X}},
keywords = {{MTHPC; Skin carcinomas; Fluorescence imaging; Fluorescence spectroscopy}},
language = {{eng}},
pages = {{940--940}},
publisher = {{International Society for Optical Engineering}},
title = {{mTHPC pharmacokinetics following topical administration}},
url = {{https://lup.lub.lu.se/search/files/2791849/2370306.pdf}},
doi = {{10.1117/12.647602}},
volume = {{6094}},
year = {{2006}},
}