Activating mutations remodel the chromatin accessibility landscape to drive distinct regulatory networks in KMT2A-rearranged acute leukemia
(2024) In HemaSphere 8(9).- Abstract
Activating FLT3 and RAS mutations commonly occur in leukemia with KMT2A-gene rearrangements (KMT2A-r). However, how these mutations cooperate with the KMT2A-r to remodel the epigenetic landscape is unknown. Using a retroviral acute myeloid leukemia (AML) mouse model driven by KMT2A::MLLT3, we show that FLT3ITD, FLT3N676K, and NRASG12D remodeled the chromatin accessibility landscape and associated transcriptional networks. Although the activating mutations shared a common core of chromatin changes, each mutation exhibits unique profiles with most opened peaks associating with enhancers in intronic or intergenic regions. Specifically, FLT3N676K and NRASG12D rewired similar chromatin... (More)
Activating FLT3 and RAS mutations commonly occur in leukemia with KMT2A-gene rearrangements (KMT2A-r). However, how these mutations cooperate with the KMT2A-r to remodel the epigenetic landscape is unknown. Using a retroviral acute myeloid leukemia (AML) mouse model driven by KMT2A::MLLT3, we show that FLT3ITD, FLT3N676K, and NRASG12D remodeled the chromatin accessibility landscape and associated transcriptional networks. Although the activating mutations shared a common core of chromatin changes, each mutation exhibits unique profiles with most opened peaks associating with enhancers in intronic or intergenic regions. Specifically, FLT3N676K and NRASG12D rewired similar chromatin and transcriptional networks, distinct from those mediated by FLT3ITD. Motif analysis uncovered a role for the AP-1 family of transcription factors in KMT2A::MLLT3 leukemia with FLT3N676K and NRASG12D, whereas Runx1 and Stat5a/Stat5b were active in the presence of FLT3ITD. Furthermore, transcriptional programs linked to immune cell regulation were activated in KMT2A-r AML expressing NRASG12D or FLT3N676K, and the expression of NKG2D-ligands on KMT2A-r cells rendered them sensitive to CAR T cell-mediated killing. Human KMT2A-r AML cells could be pharmacologically sensitized to NKG2D-CAR T cells by treatment with the histone deacetylase inhibitor LBH589 (panobinostat) which caused upregulation of NKG2D-ligand levels. Co-treatment with LBH589 and NKG2D-CAR T cells enabled robust AML cell killing, and the strongest effect was observed for cells expressing NRASG12D. Finally, the results were validated and extended to acute leukemia in infancy. Combined, activating mutations induced mutation-specific changes in the epigenetic landscape, leading to changes in transcriptional programs orchestrated by specific transcription factor networks.
(Less)
- author
- organization
-
- The pathogenetic mechanisms behind MLL-rearranged acute leukemia in infancy (research group)
- Division of Clinical Genetics
- LUCC: Lund University Cancer Centre
- Medicine/Emergency Medicine, Lund
- Diabetes (research group)
- Translational Genomic and Functional Studies of Leukemia (research group)
- LTH Profile Area: Engineering Health
- publishing date
- 2024-09
- type
- Contribution to journal
- publication status
- published
- subject
- in
- HemaSphere
- volume
- 8
- issue
- 9
- article number
- e70006
- publisher
- Wolters Kluwer
- external identifiers
-
- pmid:39329074
- scopus:85205054305
- ISSN
- 2572-9241
- DOI
- 10.1002/hem3.70006
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2024 The Author(s). HemaSphere published by John Wiley & Sons Ltd on behalf of European Hematology Association.
- id
- 6348848f-6f3b-47dc-abd7-a5a0669016d6
- date added to LUP
- 2024-12-18 14:32:08
- date last changed
- 2025-07-03 06:52:22
@article{6348848f-6f3b-47dc-abd7-a5a0669016d6, abstract = {{<p>Activating FLT3 and RAS mutations commonly occur in leukemia with KMT2A-gene rearrangements (KMT2A-r). However, how these mutations cooperate with the KMT2A-r to remodel the epigenetic landscape is unknown. Using a retroviral acute myeloid leukemia (AML) mouse model driven by KMT2A::MLLT3, we show that FLT3<sup>ITD</sup>, FLT3<sup>N676K</sup>, and NRAS<sup>G12D</sup> remodeled the chromatin accessibility landscape and associated transcriptional networks. Although the activating mutations shared a common core of chromatin changes, each mutation exhibits unique profiles with most opened peaks associating with enhancers in intronic or intergenic regions. Specifically, FLT3<sup>N676K</sup> and NRAS<sup>G12D</sup> rewired similar chromatin and transcriptional networks, distinct from those mediated by FLT3<sup>ITD</sup>. Motif analysis uncovered a role for the AP-1 family of transcription factors in KMT2A::MLLT3 leukemia with FLT3<sup>N676K</sup> and NRAS<sup>G12D</sup>, whereas Runx1 and Stat5a/Stat5b were active in the presence of FLT3<sup>ITD</sup>. Furthermore, transcriptional programs linked to immune cell regulation were activated in KMT2A-r AML expressing NRAS<sup>G12D</sup> or FLT3<sup>N676K</sup>, and the expression of NKG2D-ligands on KMT2A-r cells rendered them sensitive to CAR T cell-mediated killing. Human KMT2A-r AML cells could be pharmacologically sensitized to NKG2D-CAR T cells by treatment with the histone deacetylase inhibitor LBH589 (panobinostat) which caused upregulation of NKG2D-ligand levels. Co-treatment with LBH589 and NKG2D-CAR T cells enabled robust AML cell killing, and the strongest effect was observed for cells expressing NRAS<sup>G12D</sup>. Finally, the results were validated and extended to acute leukemia in infancy. Combined, activating mutations induced mutation-specific changes in the epigenetic landscape, leading to changes in transcriptional programs orchestrated by specific transcription factor networks.</p>}}, author = {{Zhang, Qirui and Falqués-Costa, Ton and Pilheden, Mattias and Sturesson, Helena and Ovlund, Tina and Rissler, Vendela and Castor, Anders and Marquart, Hanne V.H. and Lausen, Birgitte and Fioretos, Thoas and Hyrenius-Wittsten, Axel and Hagström-Andersson, Anna K.}}, issn = {{2572-9241}}, language = {{eng}}, number = {{9}}, publisher = {{Wolters Kluwer}}, series = {{HemaSphere}}, title = {{Activating mutations remodel the chromatin accessibility landscape to drive distinct regulatory networks in KMT2A-rearranged acute leukemia}}, url = {{http://dx.doi.org/10.1002/hem3.70006}}, doi = {{10.1002/hem3.70006}}, volume = {{8}}, year = {{2024}}, }