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The new cell irradiation facility at the Lund nuclear probe

Arteaga, Natalia LU ; Pallon, Jan LU ; Gram, Magnus LU ; Auzelyte, Vaida LU ; Elfman, Mikael LU ; Kristiansson, Per LU ; Malmqvist, Klas LU ; Nilsson, Christer LU and Wegdén, Marie LU (2007) 10th International Conference on Nuclear Microprobe Technology and Applications and 2nd International Workshop on Proton Beam Writing In Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms 260(1). p.91-96
Abstract
The CELLION project is directed towards the studies on cellular response to targeted single ions. This paper gives an account of the modifications made at the Lund sub-micron beam line in order to create a Single Ion Hit Facility for biological applications within the CELLION project. The most relevant new feature is the specially developed software used to locate the cells. A program for cell recognition and localization that gives the coordinates of the centre of the cells has been designed. The recognition is made online. A picture taken by a microscope is used as input parameter for the recognition program. Using V79 Chinese hamster cells, the recognition procedure can be done in less than 0.5 s for a picture size of 800 x 600 pixels... (More)
The CELLION project is directed towards the studies on cellular response to targeted single ions. This paper gives an account of the modifications made at the Lund sub-micron beam line in order to create a Single Ion Hit Facility for biological applications within the CELLION project. The most relevant new feature is the specially developed software used to locate the cells. A program for cell recognition and localization that gives the coordinates of the centre of the cells has been designed. The recognition is made online. A picture taken by a microscope is used as input parameter for the recognition program. Using V79 Chinese hamster cells, the recognition procedure can be done in less than 0.5 s for a picture size of 800 x 600 pixels with approximately 96% efficiency. This paper also reports target accuracy test results and the first non-targeted irradiation procedure performed at the Lund sub-micron beam line. (c) 2007 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
irradiation, single ions, cell, CELLION
in
Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms
volume
260
issue
1
pages
91 - 96
publisher
Elsevier
conference name
10th International Conference on Nuclear Microprobe Technology and Applications and 2nd International Workshop on Proton Beam Writing
external identifiers
  • wos:000248264900018
  • scopus:34249882436
ISSN
0168-583X
DOI
10.1016/j.nimb.2007.01.255
language
English
LU publication?
yes
id
f72bfcd6-e5aa-41e7-9a7d-35ddc5cd5a06 (old id 647975)
date added to LUP
2007-12-06 13:20:11
date last changed
2017-01-01 06:42:00
@article{f72bfcd6-e5aa-41e7-9a7d-35ddc5cd5a06,
  abstract     = {The CELLION project is directed towards the studies on cellular response to targeted single ions. This paper gives an account of the modifications made at the Lund sub-micron beam line in order to create a Single Ion Hit Facility for biological applications within the CELLION project. The most relevant new feature is the specially developed software used to locate the cells. A program for cell recognition and localization that gives the coordinates of the centre of the cells has been designed. The recognition is made online. A picture taken by a microscope is used as input parameter for the recognition program. Using V79 Chinese hamster cells, the recognition procedure can be done in less than 0.5 s for a picture size of 800 x 600 pixels with approximately 96% efficiency. This paper also reports target accuracy test results and the first non-targeted irradiation procedure performed at the Lund sub-micron beam line. (c) 2007 Elsevier B.V. All rights reserved.},
  author       = {Arteaga, Natalia and Pallon, Jan and Gram, Magnus and Auzelyte, Vaida and Elfman, Mikael and Kristiansson, Per and Malmqvist, Klas and Nilsson, Christer and Wegdén, Marie},
  issn         = {0168-583X},
  keyword      = {irradiation,single ions,cell,CELLION},
  language     = {eng},
  number       = {1},
  pages        = {91--96},
  publisher    = {Elsevier},
  series       = {Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms},
  title        = {The new cell irradiation facility at the Lund nuclear probe},
  url          = {http://dx.doi.org/10.1016/j.nimb.2007.01.255},
  volume       = {260},
  year         = {2007},
}