Gut microbiota composition in relation to intake of added sugar, sugar-sweetened beverages and artificially sweetened beverages in the Malmö Offspring Study
Ramne, Stina LU
; Brunkwall, Louise
LU
; Ericson, Ulrika
LU
; Gray, Nicola
; Kuhnle, Gunter G C
; Nilsson, Peter M
LU
; Orho-Melander, Marju
LU
and Sonestedt, Emily
LU
(2021)
In European Journal of Nutrition
60(4).
p.2087-2097
- Abstract
PURPOSE: It has been suggested that a high intake of sugar or sweeteners may result in an unfavorable microbiota composition; however, evidence is lacking. Hence, in this exploratory epidemiological study, we aim to examine if intake of added sugar, sugar-sweetened beverages (SSBs) or artificially sweetened beverages (ASBs) associate with the gut microbiota composition.
METHODS: Participants (18-70 years) in the Malmö Offspring Study have provided blood, urine, and fecal samples and completed both web-based 4 day food records and short food frequency questionnaires. The gut microbiota was assessed by 16S rRNA sequencing, processed in QIIME and matched to Greengenes (v.13.8), giving 64 included genera after filtering. Intake of... (More)
PURPOSE: It has been suggested that a high intake of sugar or sweeteners may result in an unfavorable microbiota composition; however, evidence is lacking. Hence, in this exploratory epidemiological study, we aim to examine if intake of added sugar, sugar-sweetened beverages (SSBs) or artificially sweetened beverages (ASBs) associate with the gut microbiota composition.
METHODS: Participants (18-70 years) in the Malmö Offspring Study have provided blood, urine, and fecal samples and completed both web-based 4 day food records and short food frequency questionnaires. The gut microbiota was assessed by 16S rRNA sequencing, processed in QIIME and matched to Greengenes (v.13.8), giving 64 included genera after filtering. Intake of added sugar (n = 1371) (also supported by the overnight urinary sugar biomarker in a subgroup n = 577), SSBs (n = 1086) and ASBs (n = 1085) were examined as exposures in negative binomial regressions.
RESULTS: Various genera nominally associated with intake of added sugar, SSBs, and ASBs. Only the negative association between SSB intake and Lachnobacterium remained significant after multiple testing correction. A positive association between SSB intake and the Firmicutes:Bacteroidetes ratio was also observed.
CONCLUSION: In this wide population, the cross-sectional associations between added sugar and sweet beverage intake and the gut microbiota are modest, but the results suggest that SSB intake is associated negatively with the genus Lachnobacterium and positively with the Firmicutes:Bacteroidetes ratio. Larger studies, preferably using metagenomic sequencing, are needed to further evaluate if a link exists between intake of sugars and sweeteners and the human gut microbiota.
(Less)
- author
- Ramne, Stina
LU
; Brunkwall, Louise
LU
; Ericson, Ulrika
LU
; Gray, Nicola
; Kuhnle, Gunter G C
; Nilsson, Peter M
LU
; Orho-Melander, Marju
LU
and Sonestedt, Emily
LU
- organization
- publishing date
- 2021-06-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- European Journal of Nutrition
- volume
- 60
- issue
- 4
- pages
- 11 pages
- publisher
- Springer
- external identifiers
-
- pmid:33030577
- scopus:85092365816
- ISSN
- 1436-6215
- DOI
- 10.1007/s00394-020-02392-0
- project
- Carbohydrate quality, microbiota and cardiometabolic risk
- Sugar consumption and cardiometabolic risk
- language
- English
- LU publication?
- yes
- id
- 65dd1b4c-b74a-45e0-848c-d205bf2267c8
- date added to LUP
- 2020-10-17 15:00:59
- date last changed
- 2024-06-12 21:28:00
@article{65dd1b4c-b74a-45e0-848c-d205bf2267c8,
abstract = {{<p>PURPOSE: It has been suggested that a high intake of sugar or sweeteners may result in an unfavorable microbiota composition; however, evidence is lacking. Hence, in this exploratory epidemiological study, we aim to examine if intake of added sugar, sugar-sweetened beverages (SSBs) or artificially sweetened beverages (ASBs) associate with the gut microbiota composition.</p><p>METHODS: Participants (18-70 years) in the Malmö Offspring Study have provided blood, urine, and fecal samples and completed both web-based 4 day food records and short food frequency questionnaires. The gut microbiota was assessed by 16S rRNA sequencing, processed in QIIME and matched to Greengenes (v.13.8), giving 64 included genera after filtering. Intake of added sugar (n = 1371) (also supported by the overnight urinary sugar biomarker in a subgroup n = 577), SSBs (n = 1086) and ASBs (n = 1085) were examined as exposures in negative binomial regressions.</p><p>RESULTS: Various genera nominally associated with intake of added sugar, SSBs, and ASBs. Only the negative association between SSB intake and Lachnobacterium remained significant after multiple testing correction. A positive association between SSB intake and the Firmicutes:Bacteroidetes ratio was also observed.</p><p>CONCLUSION: In this wide population, the cross-sectional associations between added sugar and sweet beverage intake and the gut microbiota are modest, but the results suggest that SSB intake is associated negatively with the genus Lachnobacterium and positively with the Firmicutes:Bacteroidetes ratio. Larger studies, preferably using metagenomic sequencing, are needed to further evaluate if a link exists between intake of sugars and sweeteners and the human gut microbiota.</p>}},
author = {{Ramne, Stina and Brunkwall, Louise and Ericson, Ulrika and Gray, Nicola and Kuhnle, Gunter G C and Nilsson, Peter M and Orho-Melander, Marju and Sonestedt, Emily}},
issn = {{1436-6215}},
language = {{eng}},
month = {{06}},
number = {{4}},
pages = {{2087--2097}},
publisher = {{Springer}},
series = {{European Journal of Nutrition}},
title = {{Gut microbiota composition in relation to intake of added sugar, sugar-sweetened beverages and artificially sweetened beverages in the Malmö Offspring Study}},
url = {{http://dx.doi.org/10.1007/s00394-020-02392-0}},
doi = {{10.1007/s00394-020-02392-0}},
volume = {{60}},
year = {{2021}},
}