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Chloroplast membranes retard fat digestion and induce satiety: effect of biological membranes on pancreatic lipase/co-lipase

Albertsson, Per-Åke LU ; Köhnke, Rickard LU ; Emek, Sinan Cem LU ; Mei, Jie LU ; Rehfeld, Jens F.; Åkerlund, Hans-Erik LU and Erlanson-Albertsson, Charlotte LU (2007) In Biochemical Journal 401. p.727-733
Abstract
Human obesity is a global epidemic, which causes a rapidly increased frequency of diabetes and cardiovascular disease. One reason for obesity is the ready availability of refined food products with high caloric density, an evolutionarily new event, which makes over-consumption of food inevitable. Fat is a food product with high caloric density. The mechanism for regulation of fat intake has therefore been studied to a great extent. Such studies have shown that, as long as fat stays in the intestine, satiety is promoted. This occurs through the fat-released peptide hormones, the best known being CCK (cholecystokinin), which is released by fatty acids. Hence, retarded fat digestion with prolonged time for delivery of fatty acids promotes... (More)
Human obesity is a global epidemic, which causes a rapidly increased frequency of diabetes and cardiovascular disease. One reason for obesity is the ready availability of refined food products with high caloric density, an evolutionarily new event, which makes over-consumption of food inevitable. Fat is a food product with high caloric density. The mechanism for regulation of fat intake has therefore been studied to a great extent. Such studies have shown that, as long as fat stays in the intestine, satiety is promoted. This occurs through the fat-released peptide hormones, the best known being CCK (cholecystokinin), which is released by fatty acids. Hence, retarded fat digestion with prolonged time for delivery of fatty acids promotes satiety. Pancreatic lipase, together with its protein cofactor, co-lipase, is the main enzymatic system responsible for intestinal fat digestion. We found that biological membranes, isolated from plants, animals or bacteria, inhibit the lipase/co-lipase-catalysed hydrolysis of triacylglycerols even in the presence of bile salt. We propose that the inhibition is due to binding of lipase/co-lipase to the membranes and adsorption of the membranes to the aqueous/triacylglycerol interface, thereby hindering lipase/co-lipase from acting on its lipid substrate. We also found that chloroplast membranes (thylakoids), when added to refined food, suppressed food intake in rats, lowered blood lipids and raised the satiety hormones, CCK and enterostatin. Consequently, the mechanism for satiety seems to be retardation of fat digestion allowing the fat products to stay longer in the intestine. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
co-lipase, enterostatin, cholecystokinin (CCK), blood lipid, chloroplast membrane, high-fat food intake, thylakoid
in
Biochemical Journal
volume
401
pages
727 - 733
publisher
Portland Press Limited
external identifiers
  • wos:000243982700013
  • scopus:33846869970
ISSN
0264-6021
DOI
10.1042/BJ20061463
language
English
LU publication?
yes
id
633ab708-0155-473b-91d3-a37767a4f10f (old id 675306)
date added to LUP
2007-12-04 17:17:45
date last changed
2017-04-09 04:15:33
@article{633ab708-0155-473b-91d3-a37767a4f10f,
  abstract     = {Human obesity is a global epidemic, which causes a rapidly increased frequency of diabetes and cardiovascular disease. One reason for obesity is the ready availability of refined food products with high caloric density, an evolutionarily new event, which makes over-consumption of food inevitable. Fat is a food product with high caloric density. The mechanism for regulation of fat intake has therefore been studied to a great extent. Such studies have shown that, as long as fat stays in the intestine, satiety is promoted. This occurs through the fat-released peptide hormones, the best known being CCK (cholecystokinin), which is released by fatty acids. Hence, retarded fat digestion with prolonged time for delivery of fatty acids promotes satiety. Pancreatic lipase, together with its protein cofactor, co-lipase, is the main enzymatic system responsible for intestinal fat digestion. We found that biological membranes, isolated from plants, animals or bacteria, inhibit the lipase/co-lipase-catalysed hydrolysis of triacylglycerols even in the presence of bile salt. We propose that the inhibition is due to binding of lipase/co-lipase to the membranes and adsorption of the membranes to the aqueous/triacylglycerol interface, thereby hindering lipase/co-lipase from acting on its lipid substrate. We also found that chloroplast membranes (thylakoids), when added to refined food, suppressed food intake in rats, lowered blood lipids and raised the satiety hormones, CCK and enterostatin. Consequently, the mechanism for satiety seems to be retardation of fat digestion allowing the fat products to stay longer in the intestine.},
  author       = {Albertsson, Per-Åke and Köhnke, Rickard and Emek, Sinan Cem and Mei, Jie and Rehfeld, Jens F. and Åkerlund, Hans-Erik and Erlanson-Albertsson, Charlotte},
  issn         = {0264-6021},
  keyword      = {co-lipase,enterostatin,cholecystokinin (CCK),blood lipid,chloroplast membrane,high-fat food intake,thylakoid},
  language     = {eng},
  pages        = {727--733},
  publisher    = {Portland Press Limited},
  series       = {Biochemical Journal},
  title        = {Chloroplast membranes retard fat digestion and induce satiety: effect of biological membranes on pancreatic lipase/co-lipase},
  url          = {http://dx.doi.org/10.1042/BJ20061463},
  volume       = {401},
  year         = {2007},
}