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Identification of high beta-glucan oat lines and localization and chemical characterization of their seed kernel beta-glucans

Sikora, Per; Tosh, Susan M.; Brummer, Yolanda and Olsson, Olof LU (2013) In Food Chemistry 137(1-4). p.83-91
Abstract
Oat, (Avena sativa) is an excellent source of mixed linkage beta-glucans ((1 -> 3)(1 -> 4)-beta-D-glucan), a dietary fibre with cholesterol lowering properties. Using a mutagenized oat-population we screened 1700 different lines and identified ten lines that displayed beta-glucan levels above 6.7% and 10 below 3.6%. The extreme values were 1.8% and 7.5%. We chose six lines with an increased- and four lines with a reduced beta-glucan content for further study. By longitudinal- and cross-sections of seeds it was shown that localization of beta-glucan varied between the different lines. In addition, beta-glucan quality parameters like molecular weight and solubility were also determined. Although the selection was designed for... (More)
Oat, (Avena sativa) is an excellent source of mixed linkage beta-glucans ((1 -> 3)(1 -> 4)-beta-D-glucan), a dietary fibre with cholesterol lowering properties. Using a mutagenized oat-population we screened 1700 different lines and identified ten lines that displayed beta-glucan levels above 6.7% and 10 below 3.6%. The extreme values were 1.8% and 7.5%. We chose six lines with an increased- and four lines with a reduced beta-glucan content for further study. By longitudinal- and cross-sections of seeds it was shown that localization of beta-glucan varied between the different lines. In addition, beta-glucan quality parameters like molecular weight and solubility were also determined. Although the selection was designed for quantitative differences, qualitative differences between the beta-glucans from the different lines were found. The high and low beta-glucan lines will now be used as a model system to study molecular regulation of beta-glucan biosynthesis as well as possible links between fibre quality and biological activity. (C) 2012 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Beta-glucan, Calcofluor staining, Fibre quality, Histology, Oat, Screening
in
Food Chemistry
volume
137
issue
1-4
pages
83 - 91
publisher
Elsevier
external identifiers
  • wos:000313386400012
  • scopus:84870448692
ISSN
1873-7072
DOI
10.1016/j.foodchem.2012.10.007
language
English
LU publication?
yes
id
6865b81e-7a33-4b8f-8630-2a7ca9671bf4 (old id 3470434)
date added to LUP
2013-02-25 08:10:23
date last changed
2019-02-20 05:43:19
@article{6865b81e-7a33-4b8f-8630-2a7ca9671bf4,
  abstract     = {Oat, (Avena sativa) is an excellent source of mixed linkage beta-glucans ((1 -> 3)(1 -> 4)-beta-D-glucan), a dietary fibre with cholesterol lowering properties. Using a mutagenized oat-population we screened 1700 different lines and identified ten lines that displayed beta-glucan levels above 6.7% and 10 below 3.6%. The extreme values were 1.8% and 7.5%. We chose six lines with an increased- and four lines with a reduced beta-glucan content for further study. By longitudinal- and cross-sections of seeds it was shown that localization of beta-glucan varied between the different lines. In addition, beta-glucan quality parameters like molecular weight and solubility were also determined. Although the selection was designed for quantitative differences, qualitative differences between the beta-glucans from the different lines were found. The high and low beta-glucan lines will now be used as a model system to study molecular regulation of beta-glucan biosynthesis as well as possible links between fibre quality and biological activity. (C) 2012 Elsevier Ltd. All rights reserved.},
  author       = {Sikora, Per and Tosh, Susan M. and Brummer, Yolanda and Olsson, Olof},
  issn         = {1873-7072},
  keyword      = {Beta-glucan,Calcofluor staining,Fibre quality,Histology,Oat,Screening},
  language     = {eng},
  number       = {1-4},
  pages        = {83--91},
  publisher    = {Elsevier},
  series       = {Food Chemistry},
  title        = {Identification of high beta-glucan oat lines and localization and chemical characterization of their seed kernel beta-glucans},
  url          = {http://dx.doi.org/10.1016/j.foodchem.2012.10.007},
  volume       = {137},
  year         = {2013},
}