Identification of high beta-glucan oat lines and localization and chemical characterization of their seed kernel beta-glucans
(2013) In Food Chemistry 137(1-4). p.83-91- Abstract
- Oat, (Avena sativa) is an excellent source of mixed linkage beta-glucans ((1 -> 3)(1 -> 4)-beta-D-glucan), a dietary fibre with cholesterol lowering properties. Using a mutagenized oat-population we screened 1700 different lines and identified ten lines that displayed beta-glucan levels above 6.7% and 10 below 3.6%. The extreme values were 1.8% and 7.5%. We chose six lines with an increased- and four lines with a reduced beta-glucan content for further study. By longitudinal- and cross-sections of seeds it was shown that localization of beta-glucan varied between the different lines. In addition, beta-glucan quality parameters like molecular weight and solubility were also determined. Although the selection was designed for... (More)
- Oat, (Avena sativa) is an excellent source of mixed linkage beta-glucans ((1 -> 3)(1 -> 4)-beta-D-glucan), a dietary fibre with cholesterol lowering properties. Using a mutagenized oat-population we screened 1700 different lines and identified ten lines that displayed beta-glucan levels above 6.7% and 10 below 3.6%. The extreme values were 1.8% and 7.5%. We chose six lines with an increased- and four lines with a reduced beta-glucan content for further study. By longitudinal- and cross-sections of seeds it was shown that localization of beta-glucan varied between the different lines. In addition, beta-glucan quality parameters like molecular weight and solubility were also determined. Although the selection was designed for quantitative differences, qualitative differences between the beta-glucans from the different lines were found. The high and low beta-glucan lines will now be used as a model system to study molecular regulation of beta-glucan biosynthesis as well as possible links between fibre quality and biological activity. (C) 2012 Elsevier Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3470434
- author
- Sikora, Per ; Tosh, Susan M. ; Brummer, Yolanda and Olsson, Olof LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Beta-glucan, Calcofluor staining, Fibre quality, Histology, Oat, Screening
- in
- Food Chemistry
- volume
- 137
- issue
- 1-4
- pages
- 83 - 91
- publisher
- Elsevier
- external identifiers
-
- wos:000313386400012
- scopus:84870448692
- pmid:23199994
- ISSN
- 1873-7072
- DOI
- 10.1016/j.foodchem.2012.10.007
- language
- English
- LU publication?
- yes
- id
- 6865b81e-7a33-4b8f-8630-2a7ca9671bf4 (old id 3470434)
- date added to LUP
- 2016-04-01 13:36:17
- date last changed
- 2022-04-14 02:04:31
@article{6865b81e-7a33-4b8f-8630-2a7ca9671bf4,
abstract = {{Oat, (Avena sativa) is an excellent source of mixed linkage beta-glucans ((1 -> 3)(1 -> 4)-beta-D-glucan), a dietary fibre with cholesterol lowering properties. Using a mutagenized oat-population we screened 1700 different lines and identified ten lines that displayed beta-glucan levels above 6.7% and 10 below 3.6%. The extreme values were 1.8% and 7.5%. We chose six lines with an increased- and four lines with a reduced beta-glucan content for further study. By longitudinal- and cross-sections of seeds it was shown that localization of beta-glucan varied between the different lines. In addition, beta-glucan quality parameters like molecular weight and solubility were also determined. Although the selection was designed for quantitative differences, qualitative differences between the beta-glucans from the different lines were found. The high and low beta-glucan lines will now be used as a model system to study molecular regulation of beta-glucan biosynthesis as well as possible links between fibre quality and biological activity. (C) 2012 Elsevier Ltd. All rights reserved.}},
author = {{Sikora, Per and Tosh, Susan M. and Brummer, Yolanda and Olsson, Olof}},
issn = {{1873-7072}},
keywords = {{Beta-glucan; Calcofluor staining; Fibre quality; Histology; Oat; Screening}},
language = {{eng}},
number = {{1-4}},
pages = {{83--91}},
publisher = {{Elsevier}},
series = {{Food Chemistry}},
title = {{Identification of high beta-glucan oat lines and localization and chemical characterization of their seed kernel beta-glucans}},
url = {{http://dx.doi.org/10.1016/j.foodchem.2012.10.007}},
doi = {{10.1016/j.foodchem.2012.10.007}},
volume = {{137}},
year = {{2013}},
}