Correlative imaging to resolve molecular structures in individual cells: Substrate validation study for super-resolution infrared microspectroscopy
Paulus, Agnes LU ; Yogarasa, Sahana ; Kansiz, Mustafa ; Martinsson, Isak LU ; Gouras, Gunnar K. LU
; Deierborg, Tomas
LU
; Engdahl, Anders
LU
; Borondics, Ferenc
and Klementieva, Oxana
LU
(2022)
In Nanomedicine: Nanotechnology, Biology and Medicine
43.
p.102563-102563
- Abstract
- Light microscopy has been a favorite tool of biological studies for almost a century, recently producing detailed images with exquisite molecular specificity achieving spatial resolution at nanoscale. However, light microscopy is insufficient to provide chemical information as a standalone technique. An increasing amount of evidence demonstrates that optical photothermal infrared microspectroscopy (O-PTIR) is a valuable imaging tool that can extract chemical information to locate molecular structures at submicron resolution. To further investigate the applicability of sub-micron infrared microspectroscopy for biomedical applications, we analyzed the contribution of substrate chemistry to the infrared spectra acquired from individual... (More)
- Light microscopy has been a favorite tool of biological studies for almost a century, recently producing detailed images with exquisite molecular specificity achieving spatial resolution at nanoscale. However, light microscopy is insufficient to provide chemical information as a standalone technique. An increasing amount of evidence demonstrates that optical photothermal infrared microspectroscopy (O-PTIR) is a valuable imaging tool that can extract chemical information to locate molecular structures at submicron resolution. To further investigate the applicability of sub-micron infrared microspectroscopy for biomedical applications, we analyzed the contribution of substrate chemistry to the infrared spectra acquired from individual neurons grown on various imaging substrates. To provide an example of correlative immunofluorescence/O-PTIR imaging, we used immunofluorescence to locate specific organelles for O-PTIR measurement, thus capturing molecular structures at the sub-cellular level directly in cells, which is not possible using traditional infrared microspectroscopy or immunofluorescence microscopy alone. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/6ab5e19f-9c4c-4af0-9b7b-6022a191adc0
- author
- Paulus, Agnes
LU
; Yogarasa, Sahana
; Kansiz, Mustafa
; Martinsson, Isak
LU
; Gouras, Gunnar K.
LU
; Deierborg, Tomas
LU
; Engdahl, Anders
LU
; Borondics, Ferenc
and Klementieva, Oxana
LU
- organization
- publishing date
- 2022
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Nanomedicine: Nanotechnology, Biology and Medicine
- volume
- 43
- article number
- 102563
- pages
- 102563 - 102563
- publisher
- Elsevier
- external identifiers
-
- pmid:35504462
- scopus:85129979695
- ISSN
- 1549-9642
- DOI
- 10.1016/j.nano.2022.102563
- language
- English
- LU publication?
- yes
- id
- 6ab5e19f-9c4c-4af0-9b7b-6022a191adc0
- date added to LUP
- 2022-05-13 16:49:05
- date last changed
- 2023-11-21 09:27:06
@article{6ab5e19f-9c4c-4af0-9b7b-6022a191adc0,
abstract = {{Light microscopy has been a favorite tool of biological studies for almost a century, recently producing detailed images with exquisite molecular specificity achieving spatial resolution at nanoscale. However, light microscopy is insufficient to provide chemical information as a standalone technique. An increasing amount of evidence demonstrates that optical photothermal infrared microspectroscopy (O-PTIR) is a valuable imaging tool that can extract chemical information to locate molecular structures at submicron resolution. To further investigate the applicability of sub-micron infrared microspectroscopy for biomedical applications, we analyzed the contribution of substrate chemistry to the infrared spectra acquired from individual neurons grown on various imaging substrates. To provide an example of correlative immunofluorescence/O-PTIR imaging, we used immunofluorescence to locate specific organelles for O-PTIR measurement, thus capturing molecular structures at the sub-cellular level directly in cells, which is not possible using traditional infrared microspectroscopy or immunofluorescence microscopy alone.}},
author = {{Paulus, Agnes and Yogarasa, Sahana and Kansiz, Mustafa and Martinsson, Isak and Gouras, Gunnar K. and Deierborg, Tomas and Engdahl, Anders and Borondics, Ferenc and Klementieva, Oxana}},
issn = {{1549-9642}},
language = {{eng}},
pages = {{102563--102563}},
publisher = {{Elsevier}},
series = {{Nanomedicine: Nanotechnology, Biology and Medicine}},
title = {{Correlative imaging to resolve molecular structures in individual cells: Substrate validation study for super-resolution infrared microspectroscopy}},
url = {{http://dx.doi.org/10.1016/j.nano.2022.102563}},
doi = {{10.1016/j.nano.2022.102563}},
volume = {{43}},
year = {{2022}},
}