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Exploring structure and interactions of the bacterial adaptor protein YjbH by crosslinking mass spectrometry

Al-Eryani, Yusra LU ; Ib Rasmussen, Morten; Kjellström, Sven LU ; Højrup, Peter; Emanuelsson, Cecilia LU and von Wachenfeldt, Claes LU (2016) In Proteins: Structure, Function and Genetics 84(9). p.1234-1245
Abstract

Adaptor proteins assist proteases in degrading specific proteins under appropriate conditions. The adaptor protein YjbH promotes the degradation of an important global transcriptional regulator Spx, which controls the expression of hundreds of genes and operons in response to thiol-specific oxidative stress in Bacillus subtilis. Under normal growth conditions, the transcription factor is bound to the adaptor protein and therefore degraded by the AAA+ protease ClpXP. If this binding is alleviated during stress, the transcription factor accumulates and turns on genes encoding stress-alleviating proteins. The adaptor protein YjbH is thus a key player involved in these interactions but its structure is unknown. To gain insight into its... (More)

Adaptor proteins assist proteases in degrading specific proteins under appropriate conditions. The adaptor protein YjbH promotes the degradation of an important global transcriptional regulator Spx, which controls the expression of hundreds of genes and operons in response to thiol-specific oxidative stress in Bacillus subtilis. Under normal growth conditions, the transcription factor is bound to the adaptor protein and therefore degraded by the AAA+ protease ClpXP. If this binding is alleviated during stress, the transcription factor accumulates and turns on genes encoding stress-alleviating proteins. The adaptor protein YjbH is thus a key player involved in these interactions but its structure is unknown. To gain insight into its structure and interactions we have used chemical crosslinking mass spectrometry. Distance constraints obtained from the crosslinked monomer were used to select and validate a structure model of YjbH and then to probe its interactions with other proteins. The core structure of YjbH is reminiscent of DsbA family proteins. One lysine residue in YjbH (K177), located in one of the α-helices outside the thioredoxin fold, crosslinked to both Spx K99 and Spx K117, thereby suggesting one side of the YjbH for the interaction with Spx. Another lysine residue that crosslinked to Spx was YjbH K5, located in the long and presumably very flexible N-terminal arm of YjbH. Our crosslinking data lend support to a model proposed based on site-directed mutagenesis where the YjbH interaction with Spx can stabilize and present the C-terminal region of Spx for protease recognition and proteolysis. Proteins 2016; 84:1234–1245.

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author
organization
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Contribution to journal
publication status
published
subject
keywords
Bacillus subtilis, Geobacillus kaustophilus, protein–protein interaction, proteolytic degradation, redox stress, regulatory proteins, stress response, structure prediction
in
Proteins: Structure, Function and Genetics
volume
84
issue
9
pages
12 pages
publisher
John Wiley & Sons
external identifiers
  • scopus:84977596568
  • wos:000382812900006
ISSN
0887-3585
DOI
10.1002/prot.25072
language
English
LU publication?
yes
id
6c75d308-c298-4a4a-8ab8-98efd118b530
date added to LUP
2016-11-23 09:00:24
date last changed
2017-01-08 06:02:19
@article{6c75d308-c298-4a4a-8ab8-98efd118b530,
  abstract     = {<p>Adaptor proteins assist proteases in degrading specific proteins under appropriate conditions. The adaptor protein YjbH promotes the degradation of an important global transcriptional regulator Spx, which controls the expression of hundreds of genes and operons in response to thiol-specific oxidative stress in Bacillus subtilis. Under normal growth conditions, the transcription factor is bound to the adaptor protein and therefore degraded by the AAA+ protease ClpXP. If this binding is alleviated during stress, the transcription factor accumulates and turns on genes encoding stress-alleviating proteins. The adaptor protein YjbH is thus a key player involved in these interactions but its structure is unknown. To gain insight into its structure and interactions we have used chemical crosslinking mass spectrometry. Distance constraints obtained from the crosslinked monomer were used to select and validate a structure model of YjbH and then to probe its interactions with other proteins. The core structure of YjbH is reminiscent of DsbA family proteins. One lysine residue in YjbH (K177), located in one of the α-helices outside the thioredoxin fold, crosslinked to both Spx K99 and Spx K117, thereby suggesting one side of the YjbH for the interaction with Spx. Another lysine residue that crosslinked to Spx was YjbH K5, located in the long and presumably very flexible N-terminal arm of YjbH. Our crosslinking data lend support to a model proposed based on site-directed mutagenesis where the YjbH interaction with Spx can stabilize and present the C-terminal region of Spx for protease recognition and proteolysis. Proteins 2016; 84:1234–1245.</p>},
  author       = {Al-Eryani, Yusra and Ib Rasmussen, Morten and Kjellström, Sven and Højrup, Peter and Emanuelsson, Cecilia and von Wachenfeldt, Claes},
  issn         = {0887-3585},
  keyword      = {Bacillus subtilis,Geobacillus kaustophilus,protein–protein interaction,proteolytic degradation,redox stress,regulatory proteins,stress response,structure prediction},
  language     = {eng},
  month        = {09},
  number       = {9},
  pages        = {1234--1245},
  publisher    = {John Wiley & Sons},
  series       = {Proteins: Structure, Function and Genetics},
  title        = {Exploring structure and interactions of the bacterial adaptor protein YjbH by crosslinking mass spectrometry},
  url          = {http://dx.doi.org/10.1002/prot.25072},
  volume       = {84},
  year         = {2016},
}