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Immune delineation of laryngeal papilloma reveals enhanced neutrophil associated gene profile

Sobti, Aastha LU ; Sakellariou, Christina LU orcid ; Nilsson, Maile ; Schwartz, Stefan LU ; Olofsson, Katarina ; Rydell, Roland LU ; Lindstedt, Malin LU orcid and Forslund, Ola LU (2021) In European Journal of Immunology 51(10). p.2535-2539
Abstract

Laryngeal papilloma (LP) is a rare benign disease, caused by recurrent multisite papillomas that are referred to as recurrent respiratory papillomatosis (RRP). RRP is caused primarily by two types of human papillomavirus (HPV): HPV6 and HPV11. The immune dysregulation within the microenvironment of the lesions has been shown to likely play a role in the development of RRP. The present study aimed at analyzing the transcriptional profile of immune response genes and cancer-related genes in the LP microenvironment. We used the NanoString® nCounter® analysis system to study expression of 730 genes among seven paired samples of LP and healthy laryngeal (HL) tissue. qRT-PCR and flow cytometric analysis was performed to... (More)

Laryngeal papilloma (LP) is a rare benign disease, caused by recurrent multisite papillomas that are referred to as recurrent respiratory papillomatosis (RRP). RRP is caused primarily by two types of human papillomavirus (HPV): HPV6 and HPV11. The immune dysregulation within the microenvironment of the lesions has been shown to likely play a role in the development of RRP. The present study aimed at analyzing the transcriptional profile of immune response genes and cancer-related genes in the LP microenvironment. We used the NanoString® nCounter® analysis system to study expression of 730 genes among seven paired samples of LP and healthy laryngeal (HL) tissue. qRT-PCR and flow cytometric analysis was performed to confirm identified transcripts and follow-up scores of infiltrating immune cells, respectively. In total, 113 differentially expressed transcripts were detected of which 37 showed increased expression levels and 76 decreased expression levels in the LP samples compared to the HL samples (fold change ≥ 2). Transcripts with increased expression levels included S100As (A7, A8, and A12), CEACAM1, neutrophil activation associated cytokines (IL8), chemokines (CXCL6), and IL receptors, e.g., IL4R. Transcripts with decreased expression in LP were associated with innate and adaptive immunity. Overall, HPV6 and 11 were present in 67% and 33% of the patients, respectively. There was a significant increase in neutrophils and a significant decrease in CD8+ T cells in LP. LP samples display an immune profile characterized by enhanced expression of neutrophilic markers and significantly reduced T cell-associated markers.

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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
B cells, CD8 T cells, immune profile, laryngeal papilloma, neutrophils
in
European Journal of Immunology
volume
51
issue
10
pages
2535 - 2539
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:85112438146
  • pmid:34337750
ISSN
0014-2980
DOI
10.1002/eji.202149202
language
English
LU publication?
yes
additional info
Funding Information: We thank Susanna Tarasco, Andy Forreryd and Tim Lindberg for technical assistance, and Dr. Malin Josefsson and Dr. Henrik Widegren for the samples collection. This work was financially supported by grants from the Marie Skłodowska‐Curie program CanFaster‐ Translational cancer faster forward doctoral program (744299), the Faculty of Engineering, Lund University, Lions Research Foundation Skåne (Grant ID 81204), Laryngfonden (Grant ID 40‐16), Lions Research Foundation Norrland (Grant ID 1818–925), AMP (Grant ID 19–973), Västerbotten county council C‐ALF (Grant ID 7003128) and PR2017‐0163 Swedish Childhood Cancer Fund. Graphical abstract created with BioRender.com. Publisher Copyright: © 2021 The Authors. European Journal of Immunology published by Wiley-VCH GmbH Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
id
6e5e6a8a-742e-4b68-ac8b-52217762dd00
date added to LUP
2021-09-17 10:59:47
date last changed
2024-11-17 09:09:27
@article{6e5e6a8a-742e-4b68-ac8b-52217762dd00,
  abstract     = {{<p>Laryngeal papilloma (LP) is a rare benign disease, caused by recurrent multisite papillomas that are referred to as recurrent respiratory papillomatosis (RRP). RRP is caused primarily by two types of human papillomavirus (HPV): HPV6 and HPV11. The immune dysregulation within the microenvironment of the lesions has been shown to likely play a role in the development of RRP. The present study aimed at analyzing the transcriptional profile of immune response genes and cancer-related genes in the LP microenvironment. We used the NanoString<sup>®</sup> nCounter<sup>®</sup> analysis system to study expression of 730 genes among seven paired samples of LP and healthy laryngeal (HL) tissue. qRT-PCR and flow cytometric analysis was performed to confirm identified transcripts and follow-up scores of infiltrating immune cells, respectively. In total, 113 differentially expressed transcripts were detected of which 37 showed increased expression levels and 76 decreased expression levels in the LP samples compared to the HL samples (fold change ≥ 2). Transcripts with increased expression levels included S100As (A7, A8, and A12), CEACAM1, neutrophil activation associated cytokines (IL8), chemokines (CXCL6), and IL receptors, e.g., IL4R. Transcripts with decreased expression in LP were associated with innate and adaptive immunity. Overall, HPV6 and 11 were present in 67% and 33% of the patients, respectively. There was a significant increase in neutrophils and a significant decrease in CD8<sup>+</sup> T cells in LP. LP samples display an immune profile characterized by enhanced expression of neutrophilic markers and significantly reduced T cell-associated markers.</p>}},
  author       = {{Sobti, Aastha and Sakellariou, Christina and Nilsson, Maile and Schwartz, Stefan and Olofsson, Katarina and Rydell, Roland and Lindstedt, Malin and Forslund, Ola}},
  issn         = {{0014-2980}},
  keywords     = {{B cells; CD8 T cells; immune profile; laryngeal papilloma; neutrophils}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{2535--2539}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{European Journal of Immunology}},
  title        = {{Immune delineation of laryngeal papilloma reveals enhanced neutrophil associated gene profile}},
  url          = {{http://dx.doi.org/10.1002/eji.202149202}},
  doi          = {{10.1002/eji.202149202}},
  volume       = {{51}},
  year         = {{2021}},
}