Inhibition of c-Jun phosphorylation reduces axonal outgrowth of adult rat nodose ganglia and dorsal root ganglia sensory neurons.
(2004) In Molecular and Cellular Neuroscience 27(3). p.267-279- Abstract
- The role of c-Jun activation for survival and regeneration of sensory neurons is unclear. Here we report that c-Jun N-terminal kinase (JNK)-mediated c-Jun activation is important for axonal outgrowth of sensory neurons in rat nodose and dorsal root ganglia (DRG). Peripheral severance of the vagus or the sciatic nerve resulted in a massive and rapid, but transient increase of the activated JNK (p-JNK) in neuronal nuclei, followed by c-Jun phosphorylation and activating transcription factor-3 (ATF3) induction. JNK inhibition by the selective JNK inhibitors SP600125 and (D)-JNKI1 did not affect neuronal survival in explanted or dissociated ganglia, but dramatically reduced axonal outgrowth, c-Jun activation, and ATF3 induction. Using... (More)
- The role of c-Jun activation for survival and regeneration of sensory neurons is unclear. Here we report that c-Jun N-terminal kinase (JNK)-mediated c-Jun activation is important for axonal outgrowth of sensory neurons in rat nodose and dorsal root ganglia (DRG). Peripheral severance of the vagus or the sciatic nerve resulted in a massive and rapid, but transient increase of the activated JNK (p-JNK) in neuronal nuclei, followed by c-Jun phosphorylation and activating transcription factor-3 (ATF3) induction. JNK inhibition by the selective JNK inhibitors SP600125 and (D)-JNKI1 did not affect neuronal survival in explanted or dissociated ganglia, but dramatically reduced axonal outgrowth, c-Jun activation, and ATF3 induction. Using retrograde labeling, we demonstrated that activated c-Jun (p-c-Jun) and ATF3 were associated with regenerative neurons. Taken together, our results suggest that JNK-mediated c-Jun activation is one of the first cell body reactions in response to nerve injury and that this activation and subsequent ATF3 induction are associated with axonal outgrowth. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/131015
- author
- Lindwall, Charlotta LU ; Dahlin, Lars LU ; Lundborg, Göran LU and Kanje, Martin LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Molecular and Cellular Neuroscience
- volume
- 27
- issue
- 3
- pages
- 267 - 279
- publisher
- Elsevier
- external identifiers
-
- wos:000224950000006
- pmid:15519242
- scopus:7244240786
- ISSN
- 1044-7431
- DOI
- 10.1016/j.mcn.2004.07.001
- project
- Nerve regeneration - signal transduktion mechanisms, timing and alternatives to nerve grafts
- language
- English
- LU publication?
- yes
- id
- 70074cdd-3a50-4ee9-93a4-4b35315add12 (old id 131015)
- date added to LUP
- 2016-04-01 11:41:32
- date last changed
- 2022-05-18 19:30:53
@article{70074cdd-3a50-4ee9-93a4-4b35315add12, abstract = {{The role of c-Jun activation for survival and regeneration of sensory neurons is unclear. Here we report that c-Jun N-terminal kinase (JNK)-mediated c-Jun activation is important for axonal outgrowth of sensory neurons in rat nodose and dorsal root ganglia (DRG). Peripheral severance of the vagus or the sciatic nerve resulted in a massive and rapid, but transient increase of the activated JNK (p-JNK) in neuronal nuclei, followed by c-Jun phosphorylation and activating transcription factor-3 (ATF3) induction. JNK inhibition by the selective JNK inhibitors SP600125 and (D)-JNKI1 did not affect neuronal survival in explanted or dissociated ganglia, but dramatically reduced axonal outgrowth, c-Jun activation, and ATF3 induction. Using retrograde labeling, we demonstrated that activated c-Jun (p-c-Jun) and ATF3 were associated with regenerative neurons. Taken together, our results suggest that JNK-mediated c-Jun activation is one of the first cell body reactions in response to nerve injury and that this activation and subsequent ATF3 induction are associated with axonal outgrowth.}}, author = {{Lindwall, Charlotta and Dahlin, Lars and Lundborg, Göran and Kanje, Martin}}, issn = {{1044-7431}}, language = {{eng}}, number = {{3}}, pages = {{267--279}}, publisher = {{Elsevier}}, series = {{Molecular and Cellular Neuroscience}}, title = {{Inhibition of c-Jun phosphorylation reduces axonal outgrowth of adult rat nodose ganglia and dorsal root ganglia sensory neurons.}}, url = {{http://dx.doi.org/10.1016/j.mcn.2004.07.001}}, doi = {{10.1016/j.mcn.2004.07.001}}, volume = {{27}}, year = {{2004}}, }