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Determination of short-chain fatty acids in serum by hollow fiber supported liquid membrane extraction coupled with gas chromatography

Zhao, Guohua LU ; Liu, Jing-Fu; Nyman, Margareta LU and Jönsson, Jan Åke LU (2007) In Journal of Chromatography. B 846(1-2). p.202-208
Abstract
A method based on hollow fiber supported liquid membrane extraction coupled with a gas chromatograph equipped with flame ionization detector (GC-FID) was developed for the determination of six short-chain fatty acids including acetic acid, propionic acid, i-butyric acid, n-butyric acid, i-valeric acid and n-valeric acid in serum. Hollow fiber supported liquid membrane extraction was employed for preconcentration and clean-up of the samples. The fatty acids were extracted from the acidic donor (diluted serum) into a liquid membrane formed in the wall of the hollow fiber with 10% tri-n-octylphoshphine oxide (TOPO) in di-n-hexyl ether, and then extracted back into a basic acceptor solution filled in the lumen of the hollow fiber. After being... (More)
A method based on hollow fiber supported liquid membrane extraction coupled with a gas chromatograph equipped with flame ionization detector (GC-FID) was developed for the determination of six short-chain fatty acids including acetic acid, propionic acid, i-butyric acid, n-butyric acid, i-valeric acid and n-valeric acid in serum. Hollow fiber supported liquid membrane extraction was employed for preconcentration and clean-up of the samples. The fatty acids were extracted from the acidic donor (diluted serum) into a liquid membrane formed in the wall of the hollow fiber with 10% tri-n-octylphoshphine oxide (TOPO) in di-n-hexyl ether, and then extracted back into a basic acceptor solution filled in the lumen of the hollow fiber. After being acidified with HCl, the acceptor was directly analyzed by GC-FID. The acceptor concentration, donor pH, membrane liquid and extracting time were optimized giving an enrichment factor up to 155 times. The good linearity (r2 > 0.980), reasonable recovery (87.2-121%), and satisfactory intra-assay (8.2-11.5%) and inter-assay (6.1-11.6%) precision illustrated the good performance of the present method. Limits of detection (LOD) ranged from 0.04 to 0.24 μM and limits of quantification (LOQ) varied from 0.13 to 0.80 μM. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Quantitative analysis, Gas chromatography, Short chain, Determination, Fatty acids, Lipids, Biological fluid
in
Journal of Chromatography. B
volume
846
issue
1-2
pages
202 - 208
publisher
Elsevier
external identifiers
  • wos:000244281100027
  • scopus:33846439032
ISSN
1873-376X
DOI
10.1016/j.jchromb.2006.09.027
language
English
LU publication?
yes
id
028908bb-f577-4ce1-86f2-d3745518c45f (old id 757476)
date added to LUP
2007-12-21 12:58:48
date last changed
2017-08-27 04:18:41
@article{028908bb-f577-4ce1-86f2-d3745518c45f,
  abstract     = {A method based on hollow fiber supported liquid membrane extraction coupled with a gas chromatograph equipped with flame ionization detector (GC-FID) was developed for the determination of six short-chain fatty acids including acetic acid, propionic acid, i-butyric acid, n-butyric acid, i-valeric acid and n-valeric acid in serum. Hollow fiber supported liquid membrane extraction was employed for preconcentration and clean-up of the samples. The fatty acids were extracted from the acidic donor (diluted serum) into a liquid membrane formed in the wall of the hollow fiber with 10% tri-n-octylphoshphine oxide (TOPO) in di-n-hexyl ether, and then extracted back into a basic acceptor solution filled in the lumen of the hollow fiber. After being acidified with HCl, the acceptor was directly analyzed by GC-FID. The acceptor concentration, donor pH, membrane liquid and extracting time were optimized giving an enrichment factor up to 155 times. The good linearity (r2 > 0.980), reasonable recovery (87.2-121%), and satisfactory intra-assay (8.2-11.5%) and inter-assay (6.1-11.6%) precision illustrated the good performance of the present method. Limits of detection (LOD) ranged from 0.04 to 0.24 μM and limits of quantification (LOQ) varied from 0.13 to 0.80 μM.},
  author       = {Zhao, Guohua and Liu, Jing-Fu and Nyman, Margareta and Jönsson, Jan Åke},
  issn         = {1873-376X},
  keyword      = {Quantitative analysis,Gas chromatography,Short chain,Determination,Fatty acids,Lipids,Biological fluid},
  language     = {eng},
  number       = {1-2},
  pages        = {202--208},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography. B},
  title        = {Determination of short-chain fatty acids in serum by hollow fiber supported liquid membrane extraction coupled with gas chromatography},
  url          = {http://dx.doi.org/10.1016/j.jchromb.2006.09.027},
  volume       = {846},
  year         = {2007},
}