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Mechanisms of adaptation to nitrosative stress in Bacillus subtilis

Rogstam, Annika LU ; Larsson, Jonas LU ; Kjelgaard, Peter LU and von Wachenfeldt, Claes LU (2007) In Journal of Bacteriology 189(8). p.3063-3071
Abstract
Bacteria use a number of mechanisms for coping with the toxic effects exerted by nitric oxide (NO) and its derivatives. Here we show that the flavohemoglobin encoded by the hmp gene has a vital role in an adaptive response to protect the soil bacterium Bacillus subtilis from nitrosative stress. We further show that nitrosative stress induced by the nitrosonium cation donor sodium nitroprusside (SNP) leads to deactivation of the transcriptional repressor NsrR, resulting in derepression of hmp. Nitrosative stress induces the sigma B-controlled general stress regulon. However, a sigB null mutant did not show increased sensitivity to SNP, suggesting that the sigma B-dependent stress proteins are involved in a nonspecific protection against... (More)
Bacteria use a number of mechanisms for coping with the toxic effects exerted by nitric oxide (NO) and its derivatives. Here we show that the flavohemoglobin encoded by the hmp gene has a vital role in an adaptive response to protect the soil bacterium Bacillus subtilis from nitrosative stress. We further show that nitrosative stress induced by the nitrosonium cation donor sodium nitroprusside (SNP) leads to deactivation of the transcriptional repressor NsrR, resulting in derepression of hmp. Nitrosative stress induces the sigma B-controlled general stress regulon. However, a sigB null mutant did not show increased sensitivity to SNP, suggesting that the sigma B-dependent stress proteins are involved in a nonspecific protection against stress whereas the Hmp flavohemoglobin plays a central role in detoxification. Mutations in the yjbIH operon, which encodes a truncated hemoglobin (YjbI) and a predicted 34-kDa cytosolic protein of unknown function (YjbH), rendered B. subtilis hypersensitive to SNP, suggesting roles in nitrosative stress management. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Bacteriology
volume
189
issue
8
pages
3063 - 3071
publisher
American Society for Microbiology
external identifiers
  • wos:000246029300013
  • scopus:34247893903
ISSN
0021-9193
DOI
10.1128/JB.01782-06
language
English
LU publication?
yes
id
b934b2b3-a5c6-4edc-b815-40c3fe1ef9c9 (old id 764769)
date added to LUP
2007-12-18 11:25:39
date last changed
2017-05-07 03:28:45
@article{b934b2b3-a5c6-4edc-b815-40c3fe1ef9c9,
  abstract     = {Bacteria use a number of mechanisms for coping with the toxic effects exerted by nitric oxide (NO) and its derivatives. Here we show that the flavohemoglobin encoded by the hmp gene has a vital role in an adaptive response to protect the soil bacterium Bacillus subtilis from nitrosative stress. We further show that nitrosative stress induced by the nitrosonium cation donor sodium nitroprusside (SNP) leads to deactivation of the transcriptional repressor NsrR, resulting in derepression of hmp. Nitrosative stress induces the sigma B-controlled general stress regulon. However, a sigB null mutant did not show increased sensitivity to SNP, suggesting that the sigma B-dependent stress proteins are involved in a nonspecific protection against stress whereas the Hmp flavohemoglobin plays a central role in detoxification. Mutations in the yjbIH operon, which encodes a truncated hemoglobin (YjbI) and a predicted 34-kDa cytosolic protein of unknown function (YjbH), rendered B. subtilis hypersensitive to SNP, suggesting roles in nitrosative stress management.},
  author       = {Rogstam, Annika and Larsson, Jonas and Kjelgaard, Peter and von Wachenfeldt, Claes},
  issn         = {0021-9193},
  language     = {eng},
  number       = {8},
  pages        = {3063--3071},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Bacteriology},
  title        = {Mechanisms of adaptation to nitrosative stress in Bacillus subtilis},
  url          = {http://dx.doi.org/10.1128/JB.01782-06},
  volume       = {189},
  year         = {2007},
}