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Analysis of nanoparticle-protein coronas formed in vitro between nanosized welding particles and nasal lavage proteins.

Ali, Neserin LU ; Mattsson, Karin; Rissler, Jenny; Karlsson, Helen Marg; Svensson, Christian R; Gudmundsson, Anders; Lindh, Christian LU ; Jönsson, Bo A LU ; Cedervall, Tommy and Kåredal, Monica LU (2015) In Nanotoxicology 10(2). p.226-234
Abstract
Welding fumes include agglomerated particles built up of primary nanoparticles. Particles inhaled through the nose will to some extent be deposited in the protein-rich nasal mucosa, and a protein corona will be formed around the particles. The aim was to identify the protein corona formed between nasal lavage proteins and four types of particles with different parameters. Two of the particles were formed and collected during welding and two were manufactured iron oxides. When nasal lavage proteins were added to the particles, differences were observed in the sizes of the aggregates that were formed. Measurements showed that the amount of protein bound to particles correlated with the relative size increase of the aggregates, suggesting... (More)
Welding fumes include agglomerated particles built up of primary nanoparticles. Particles inhaled through the nose will to some extent be deposited in the protein-rich nasal mucosa, and a protein corona will be formed around the particles. The aim was to identify the protein corona formed between nasal lavage proteins and four types of particles with different parameters. Two of the particles were formed and collected during welding and two were manufactured iron oxides. When nasal lavage proteins were added to the particles, differences were observed in the sizes of the aggregates that were formed. Measurements showed that the amount of protein bound to particles correlated with the relative size increase of the aggregates, suggesting that the surface area was associated with the binding capacity. However, differences in aggregate sizes were detected when nasal proteins were added to UFWF and Fe2O3 particles (having similar agglomerated size) suggesting that yet parameters other than size determine the binding. Relative quantitative mass spectrometric and gel-based analyses showed differences in the protein content of the coronas. High-affinity proteins were further assessed for network interactions. Additional experiments showed that the inhibitory function of secretory leukocyte peptidase inhibitor, a highly abundant nasal protein, was influenced by particle binding suggesting that an understanding of protein function following particle binding is necessary to properly evaluate pathophysiological events. Our results underscore the importance of including particles collected from real working environments when studying the toxic effects of particles because these effects might be mediated by the protein corona. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Nanotoxicology
volume
10
issue
2
pages
226 - 234
publisher
Informa Healthcare
external identifiers
  • pmid:26186033
  • scopus:84959319846
  • wos:000371822800010
ISSN
1743-5404
DOI
10.3109/17435390.2015.1048324
language
English
LU publication?
yes
id
9b750bc0-b603-45c1-bf9c-688493d97db5 (old id 7749259)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26186033?dopt=Abstract
date added to LUP
2015-08-10 00:21:58
date last changed
2017-04-30 15:34:56
@article{9b750bc0-b603-45c1-bf9c-688493d97db5,
  abstract     = {Welding fumes include agglomerated particles built up of primary nanoparticles. Particles inhaled through the nose will to some extent be deposited in the protein-rich nasal mucosa, and a protein corona will be formed around the particles. The aim was to identify the protein corona formed between nasal lavage proteins and four types of particles with different parameters. Two of the particles were formed and collected during welding and two were manufactured iron oxides. When nasal lavage proteins were added to the particles, differences were observed in the sizes of the aggregates that were formed. Measurements showed that the amount of protein bound to particles correlated with the relative size increase of the aggregates, suggesting that the surface area was associated with the binding capacity. However, differences in aggregate sizes were detected when nasal proteins were added to UFWF and Fe2O3 particles (having similar agglomerated size) suggesting that yet parameters other than size determine the binding. Relative quantitative mass spectrometric and gel-based analyses showed differences in the protein content of the coronas. High-affinity proteins were further assessed for network interactions. Additional experiments showed that the inhibitory function of secretory leukocyte peptidase inhibitor, a highly abundant nasal protein, was influenced by particle binding suggesting that an understanding of protein function following particle binding is necessary to properly evaluate pathophysiological events. Our results underscore the importance of including particles collected from real working environments when studying the toxic effects of particles because these effects might be mediated by the protein corona.},
  author       = {Ali, Neserin and Mattsson, Karin and Rissler, Jenny and Karlsson, Helen Marg and Svensson, Christian R and Gudmundsson, Anders and Lindh, Christian and Jönsson, Bo A and Cedervall, Tommy and Kåredal, Monica},
  issn         = {1743-5404},
  language     = {eng},
  number       = {2},
  pages        = {226--234},
  publisher    = {Informa Healthcare},
  series       = {Nanotoxicology},
  title        = {Analysis of nanoparticle-protein coronas formed in vitro between nanosized welding particles and nasal lavage proteins.},
  url          = {http://dx.doi.org/10.3109/17435390.2015.1048324},
  volume       = {10},
  year         = {2015},
}