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Haemophilus influenzae P4 interacts with extracellular matrix proteins promoting adhesion and serum resistance.

Su, Shanice YC LU ; Mukherjee, Oindrilla LU ; Singh, Birendra LU ; Hallgren, Oskar LU ; Westergren-Thorsson, Gunilla LU ; Hood, Derek and Riesbeck, Kristian LU (2016) In Journal of Infectious Diseases 213(2). p.314-323
Abstract
Interaction with the extracellular matrix (ECM) is one of the successful colonization strategies employed by non-typeable Haemophilus influenzae (NTHi). Here we identified Haemophilus lipoprotein 4 (P4) as a receptor for ECM proteins. Purified recombinant P4 displayed a high binding affinity for laminin (Kd=9.26 nM) and fibronectin (Kd=10.19 nM), but slightly less to vitronectin (Kd=16.51 nM). A P4-deficient NTHi mutant showed a significantly decreased binding to these ECM components. Vitronectin acquisition conferred serum resistance to both P4-expressing NTHi and Escherichia coli transformants. P4-mediated bacterial adherence to pharynx, type II alveolar and bronchial epithelial cells was mainly attributed to fibronectin. Importantly, a... (More)
Interaction with the extracellular matrix (ECM) is one of the successful colonization strategies employed by non-typeable Haemophilus influenzae (NTHi). Here we identified Haemophilus lipoprotein 4 (P4) as a receptor for ECM proteins. Purified recombinant P4 displayed a high binding affinity for laminin (Kd=9.26 nM) and fibronectin (Kd=10.19 nM), but slightly less to vitronectin (Kd=16.51 nM). A P4-deficient NTHi mutant showed a significantly decreased binding to these ECM components. Vitronectin acquisition conferred serum resistance to both P4-expressing NTHi and Escherichia coli transformants. P4-mediated bacterial adherence to pharynx, type II alveolar and bronchial epithelial cells was mainly attributed to fibronectin. Importantly, a significantly reduced bacterial infection was observed in the middle ear of the Junbo mouse model when NTHi was devoid of P4. In conclusion, our data provide new insight into the role of P4 as an important factor for Haemophilus colonization and subsequent respiratory tract infection. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Journal of Infectious Diseases
volume
213
issue
2
pages
314 - 323
publisher
Oxford University Press
external identifiers
  • PMID:26153407
  • Scopus:84959924526
  • WOS:000371237900019
ISSN
1537-6613
DOI
10.1093/infdis/jiv374
language
English
LU publication?
yes
id
d032a256-62ad-4355-bca0-c4b978846304 (old id 7750162)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26153407?dopt=Abstract
date added to LUP
2015-08-07 00:18:05
date last changed
2017-01-01 07:50:29
@article{d032a256-62ad-4355-bca0-c4b978846304,
  abstract     = {Interaction with the extracellular matrix (ECM) is one of the successful colonization strategies employed by non-typeable Haemophilus influenzae (NTHi). Here we identified Haemophilus lipoprotein 4 (P4) as a receptor for ECM proteins. Purified recombinant P4 displayed a high binding affinity for laminin (Kd=9.26 nM) and fibronectin (Kd=10.19 nM), but slightly less to vitronectin (Kd=16.51 nM). A P4-deficient NTHi mutant showed a significantly decreased binding to these ECM components. Vitronectin acquisition conferred serum resistance to both P4-expressing NTHi and Escherichia coli transformants. P4-mediated bacterial adherence to pharynx, type II alveolar and bronchial epithelial cells was mainly attributed to fibronectin. Importantly, a significantly reduced bacterial infection was observed in the middle ear of the Junbo mouse model when NTHi was devoid of P4. In conclusion, our data provide new insight into the role of P4 as an important factor for Haemophilus colonization and subsequent respiratory tract infection.},
  author       = {Su, Shanice YC and Mukherjee, Oindrilla and Singh, Birendra and Hallgren, Oskar and Westergren-Thorsson, Gunilla and Hood, Derek and Riesbeck, Kristian},
  issn         = {1537-6613},
  language     = {eng},
  number       = {2},
  pages        = {314--323},
  publisher    = {Oxford University Press},
  series       = {Journal of Infectious Diseases},
  title        = {Haemophilus influenzae P4 interacts with extracellular matrix proteins promoting adhesion and serum resistance.},
  url          = {http://dx.doi.org/10.1093/infdis/jiv374},
  volume       = {213},
  year         = {2016},
}