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A simplified hollow-fibre supported liquid membrane extraction method for quantification of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in urine and plasma samples

Lezamiz, Jon LU ; Barri, Thaer LU ; Jönsson, Jan Åke LU and Skog, Kerstin LU (2008) In Analytical and Bioanalytical Chemistry 390. p.689-696
Abstract
A simple and easy-to-use extraction procedure has been optimised, validated, and applied for extraction of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in urine and spiked plasma samples. PhIP is a carcinogenic and mutagenic heterocyclic aromatic amine that is formed during cooking of meat and fish. The novelty of the extraction procedure lies in using a short piece of narrow capillary-like microporous hollow-fibre (HF) membrane as extraction device. The HF membrane was filled with a few microlitres of acidic solution and the membrane pores were impregnated with an organic extraction solvent. Therefore, the technique was called hollow-fibre supported liquid membrane (HF-SLM) extraction. The HF extraction device was then supported... (More)
A simple and easy-to-use extraction procedure has been optimised, validated, and applied for extraction of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in urine and spiked plasma samples. PhIP is a carcinogenic and mutagenic heterocyclic aromatic amine that is formed during cooking of meat and fish. The novelty of the extraction procedure lies in using a short piece of narrow capillary-like microporous hollow-fibre (HF) membrane as extraction device. The HF membrane was filled with a few microlitres of acidic solution and the membrane pores were impregnated with an organic extraction solvent. Therefore, the technique was called hollow-fibre supported liquid membrane (HF-SLM) extraction. The HF extraction device was then supported by a syringe needle and directly immersed in urine (1.4 mL) or plasma (0.3 mL) previously made alkaline by adding 0.5 mol L−1 NaOH solution to give a final volume of 1.6 mL. The operation of the HF-SLM extraction at the optimal conditions resulted in a PhIP extraction efficiency of 74% from both spiked urine and plasma, corresponding to enrichment factors of 126 and 27, respectively. For 90 min extraction time, limits of detection and quantification were, respectively, 8 and 25 pg mL−1 for urine and 6 and 11 pg mL−1 for plasma. Within-day repeatability (n = 6) and between-day reproducibility (n = 3) were, respectively, 5% and 13% for urine and 6% and 7% for plasma. Analysis of urine samples collected for 12 h after a volunteer had eaten 250 g well-done chicken showed the PhIP concentration was 124 ± 21 pg mL−1, calculated assuming an extraction efficiency of 74%. (Less)
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publication status
published
subject
keywords
Heterocyclic aromatic amines - 2-Amino-1-methyl-6-phenylimidazo[4, 5-b]pyridine (PhIP) - Plasma - Urine - Hollow-fibre supported liquid membrane extraction
in
Analytical and Bioanalytical Chemistry
volume
390
pages
689 - 696
publisher
Springer
external identifiers
  • wos:000252170400025
  • scopus:37749044494
ISSN
1618-2642
DOI
10.1007/s00216-007-1702-4
language
English
LU publication?
yes
id
9e97f1e3-24ba-4239-84d7-f31dab4b2789 (old id 779512)
date added to LUP
2007-12-20 18:51:23
date last changed
2017-01-01 04:26:28
@article{9e97f1e3-24ba-4239-84d7-f31dab4b2789,
  abstract     = {A simple and easy-to-use extraction procedure has been optimised, validated, and applied for extraction of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in urine and spiked plasma samples. PhIP is a carcinogenic and mutagenic heterocyclic aromatic amine that is formed during cooking of meat and fish. The novelty of the extraction procedure lies in using a short piece of narrow capillary-like microporous hollow-fibre (HF) membrane as extraction device. The HF membrane was filled with a few microlitres of acidic solution and the membrane pores were impregnated with an organic extraction solvent. Therefore, the technique was called hollow-fibre supported liquid membrane (HF-SLM) extraction. The HF extraction device was then supported by a syringe needle and directly immersed in urine (1.4 mL) or plasma (0.3 mL) previously made alkaline by adding 0.5 mol L−1 NaOH solution to give a final volume of 1.6 mL. The operation of the HF-SLM extraction at the optimal conditions resulted in a PhIP extraction efficiency of 74% from both spiked urine and plasma, corresponding to enrichment factors of 126 and 27, respectively. For 90 min extraction time, limits of detection and quantification were, respectively, 8 and 25 pg mL−1 for urine and 6 and 11 pg mL−1 for plasma. Within-day repeatability (n = 6) and between-day reproducibility (n = 3) were, respectively, 5% and 13% for urine and 6% and 7% for plasma. Analysis of urine samples collected for 12 h after a volunteer had eaten 250 g well-done chicken showed the PhIP concentration was 124 ± 21 pg mL−1, calculated assuming an extraction efficiency of 74%.},
  author       = {Lezamiz, Jon and Barri, Thaer and Jönsson, Jan Åke and Skog, Kerstin},
  issn         = {1618-2642},
  keyword      = {Heterocyclic aromatic amines - 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) - Plasma - Urine - Hollow-fibre supported liquid membrane extraction},
  language     = {eng},
  pages        = {689--696},
  publisher    = {Springer},
  series       = {Analytical and Bioanalytical Chemistry},
  title        = {A simplified hollow-fibre supported liquid membrane extraction method for quantification of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in urine and plasma samples},
  url          = {http://dx.doi.org/10.1007/s00216-007-1702-4},
  volume       = {390},
  year         = {2008},
}