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Correlative optical photothermal infrared and X-ray fluorescence for chemical imaging of trace elements and relevant molecular structures directly in neurons

Gustavsson, Nadja LU ; Paulus, Agnes LU ; Martinsson, Isak LU ; Engdahl, Anders LU ; Medjoubi, Kadda ; Klementiev, Konstantin LU ; Somogyi, Andrea ; Deierborg, Tomas LU ; Borondics, Ferenc and Keppler Gouras, Gunnar LU orcid , et al. (2021) In Light, science & applications 10. p.1-10
Abstract
Alzheimer’s disease (AD) is the most common cause of dementia, costing about 1% of the global economy. Failures ofclinical trials targeting amyloid-βprotein (Aβ), a key trigger of AD, have been explained by drug inefficiency regardlessof the mechanisms of amyloid neurotoxicity, which are very difficult to address by available technologies. Here, wecombine two imaging modalities that stand at opposite ends of the electromagnetic spectrum, and therefore, can beused as complementary tools to assess structural and chemical information directly in a single neuron. Combininglabel-free super-resolution microspectroscopy for sub-cellular imaging based on novel optical photothermal infrared(O-PTIR) and synchrotron-based X-rayfluorescence (S-XRF)... (More)
Alzheimer’s disease (AD) is the most common cause of dementia, costing about 1% of the global economy. Failures ofclinical trials targeting amyloid-βprotein (Aβ), a key trigger of AD, have been explained by drug inefficiency regardlessof the mechanisms of amyloid neurotoxicity, which are very difficult to address by available technologies. Here, wecombine two imaging modalities that stand at opposite ends of the electromagnetic spectrum, and therefore, can beused as complementary tools to assess structural and chemical information directly in a single neuron. Combininglabel-free super-resolution microspectroscopy for sub-cellular imaging based on novel optical photothermal infrared(O-PTIR) and synchrotron-based X-rayfluorescence (S-XRF) nano-imaging techniques, we capture elementaldistribution andfibrillary forms of amyloid-βproteins in the same neurons at an unprecedented resolution. Our resultsreveal that in primary AD-like neurons, iron clusters co-localize with elevated amyloidβ-sheet structures and oxidizedlipids. Overall, our O-PTIR/S-XRF results motivate using high-resolution multimodal microspectroscopic approaches tounderstand the role of molecular structures and trace elements within a single neuronal cell. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Light, science & applications
volume
10
article number
151
pages
10 pages
publisher
Nature Publishing Group
external identifiers
  • scopus:85111086933
  • pmid:34294676
ISSN
2047-7538
DOI
10.1038/s41377-021-00590-x
language
English
LU publication?
yes
id
7a9efb1b-aac8-47fa-8db1-414073399f59
date added to LUP
2021-07-23 12:14:16
date last changed
2023-11-08 16:40:09
@article{7a9efb1b-aac8-47fa-8db1-414073399f59,
  abstract     = {{Alzheimer’s disease (AD) is the most common cause of dementia, costing about 1% of the global economy. Failures ofclinical trials targeting amyloid-βprotein (Aβ), a key trigger of AD, have been explained by drug inefficiency regardlessof the mechanisms of amyloid neurotoxicity, which are very difficult to address by available technologies. Here, wecombine two imaging modalities that stand at opposite ends of the electromagnetic spectrum, and therefore, can beused as complementary tools to assess structural and chemical information directly in a single neuron. Combininglabel-free super-resolution microspectroscopy for sub-cellular imaging based on novel optical photothermal infrared(O-PTIR) and synchrotron-based X-rayfluorescence (S-XRF) nano-imaging techniques, we capture elementaldistribution andfibrillary forms of amyloid-βproteins in the same neurons at an unprecedented resolution. Our resultsreveal that in primary AD-like neurons, iron clusters co-localize with elevated amyloidβ-sheet structures and oxidizedlipids. Overall, our O-PTIR/S-XRF results motivate using high-resolution multimodal microspectroscopic approaches tounderstand the role of molecular structures and trace elements within a single neuronal cell.}},
  author       = {{Gustavsson, Nadja and Paulus, Agnes and Martinsson, Isak and Engdahl, Anders and Medjoubi, Kadda and Klementiev, Konstantin and Somogyi, Andrea and Deierborg, Tomas and Borondics, Ferenc and Keppler Gouras, Gunnar and Klementieva, Oxana}},
  issn         = {{2047-7538}},
  language     = {{eng}},
  month        = {{07}},
  pages        = {{1--10}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Light, science & applications}},
  title        = {{Correlative optical photothermal infrared and X-ray fluorescence for chemical imaging of trace elements and relevant molecular structures directly in neurons}},
  url          = {{http://dx.doi.org/10.1038/s41377-021-00590-x}},
  doi          = {{10.1038/s41377-021-00590-x}},
  volume       = {{10}},
  year         = {{2021}},
}