Advanced

Sutures alone are sufficient to support regeneration across a gap in the continuity of the sciatic nerve in rats

Scherman, Peter LU ; Lundborg, Göran LU ; Kanje, Martin LU and Dahlin, Lars B. LU (2000) In Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery 34(1). p.1-8
Abstract

We have proposed that it is sufficient to provide a simple substratum on which regenerating axone may traverse a gap in a peripheral nerve. To test this hypothesis we set up a new experimental model in which sutures where used to bridge a 10 mm long defect in a peripheral nerve. A defect was created bilaterally in 25 rat sciatic nerves. The cut ends of the nerve were joined by three laps of a continuous suture, on one side with 8/0 polyamide (non-absorbable) and on the other with 8/0 polyglactin (absorbable), leaving a 7 mm gap. At two weeks a matrix that contained capillaries, fibroblast-like cells, and mononuclear cells had formed between the nerve endings, and the sutures were surrounded by foreign-body-like tissue reactions. At four... (More)

We have proposed that it is sufficient to provide a simple substratum on which regenerating axone may traverse a gap in a peripheral nerve. To test this hypothesis we set up a new experimental model in which sutures where used to bridge a 10 mm long defect in a peripheral nerve. A defect was created bilaterally in 25 rat sciatic nerves. The cut ends of the nerve were joined by three laps of a continuous suture, on one side with 8/0 polyamide (non-absorbable) and on the other with 8/0 polyglactin (absorbable), leaving a 7 mm gap. At two weeks a matrix that contained capillaries, fibroblast-like cells, and mononuclear cells had formed between the nerve endings, and the sutures were surrounded by foreign-body-like tissue reactions. At four weeks axone had grown into the distal nerve segment on both sides in 65%-90% of the cases as indicated by a response to the pinch reflex test and immunocytochemistry for presence of neurofilament protein. Axons were organised in minifascicles and these tended to grow larger as the demarcation of the entire regenerated segment by a perineurial-like structure improved with time. At 12 weeks axonal counts of cross-sections of the distal tibial trunk showed many myelinated nerve fibres but no significant difference in axonal counts or degree of myelination between the polyamide and polyglactin sides. The results show that conventional sutures alone are sufficient to support regeneration across a short gap in a peripheral nerve, a method that may be of potential clinical value.

(Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Absorbable sutures, Axonal growth, Fibrin matrix, Nerve regeneration, Nerve repair, Non-absorbable sutures, Sciatic nerve
in
Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery
volume
34
issue
1
pages
1 - 8
publisher
Taylor & Francis
external identifiers
  • scopus:0033930253
  • pmid:10756569
ISSN
0284-4311
DOI
10.1080/02844310050160105
language
English
LU publication?
yes
id
7dd7c777-50cd-40de-868f-48a53b3877ce
date added to LUP
2019-06-17 13:50:20
date last changed
2021-07-13 03:54:14
@article{7dd7c777-50cd-40de-868f-48a53b3877ce,
  abstract     = {<p>We have proposed that it is sufficient to provide a simple substratum on which regenerating axone may traverse a gap in a peripheral nerve. To test this hypothesis we set up a new experimental model in which sutures where used to bridge a 10 mm long defect in a peripheral nerve. A defect was created bilaterally in 25 rat sciatic nerves. The cut ends of the nerve were joined by three laps of a continuous suture, on one side with 8/0 polyamide (non-absorbable) and on the other with 8/0 polyglactin (absorbable), leaving a 7 mm gap. At two weeks a matrix that contained capillaries, fibroblast-like cells, and mononuclear cells had formed between the nerve endings, and the sutures were surrounded by foreign-body-like tissue reactions. At four weeks axone had grown into the distal nerve segment on both sides in 65%-90% of the cases as indicated by a response to the pinch reflex test and immunocytochemistry for presence of neurofilament protein. Axons were organised in minifascicles and these tended to grow larger as the demarcation of the entire regenerated segment by a perineurial-like structure improved with time. At 12 weeks axonal counts of cross-sections of the distal tibial trunk showed many myelinated nerve fibres but no significant difference in axonal counts or degree of myelination between the polyamide and polyglactin sides. The results show that conventional sutures alone are sufficient to support regeneration across a short gap in a peripheral nerve, a method that may be of potential clinical value.</p>},
  author       = {Scherman, Peter and Lundborg, Göran and Kanje, Martin and Dahlin, Lars B.},
  issn         = {0284-4311},
  language     = {eng},
  number       = {1},
  pages        = {1--8},
  publisher    = {Taylor & Francis},
  series       = {Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery},
  title        = {Sutures alone are sufficient to support regeneration across a gap in the continuity of the sciatic nerve in rats},
  url          = {http://dx.doi.org/10.1080/02844310050160105},
  doi          = {10.1080/02844310050160105},
  volume       = {34},
  year         = {2000},
}