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mTHPC pharmacokinetics following topical administration

Johansson, Ann LU ; Svensson, Jenny LU ; Andersson-Engels, Stefan LU ; Bendsöe, Niels LU ; Svanberg, Katarina LU ; Bigio, Irving; Alexandratou, Eleni; Kyriazi, Maria; Yova, Dido and Grafe, Susanna, et al. (2006) Optical Diagnostics and Sensing VI 6094. p.940-940
Abstract
Measurements of concentration of sensitizers for photodynamic therapy can provide important information in the dosimetry planning and can also give input to the optimal time for treatment. There has been skepticism towards fluorescence techniques for this purpose, as the signal depends on the fluorescence yield and optical properties of the tissue. Absorption based techniques, lack on the other hand, often the sensitivity required for many sensitizers with relative weak absorption in a wavelength region where hemoglobin absorption is dominant. A direct comparison between absorption and fluorescence techniques for measuring mTHPC concentration after topical application on hairless SKH-1 mice bearing skin carcinomas has been performed. 20... (More)
Measurements of concentration of sensitizers for photodynamic therapy can provide important information in the dosimetry planning and can also give input to the optimal time for treatment. There has been skepticism towards fluorescence techniques for this purpose, as the signal depends on the fluorescence yield and optical properties of the tissue. Absorption based techniques, lack on the other hand, often the sensitivity required for many sensitizers with relative weak absorption in a wavelength region where hemoglobin absorption is dominant. A direct comparison between absorption and fluorescence techniques for measuring mTHPC concentration after topical application on hairless SKH-1 mice bearing skin carcinomas has been performed. 20 μl/cm<sup>2</sup> of m-THPC thermogel (0.5 mg m-THPC/ml) were applied on normal and tumor area and the concentration of mTHPC was measured at 4 and 6 hours after drug application by two methods: 1. A fluorescence imaging system capturing images at two wavelengths (500 and 650 nm) following 405 nm excitation. Signals from different regions of interest were averaged and the intensity ratio at 650 to 500 was calculated. 2. A diffuse reflectance spectroscopy system with a fiber separation of 2 mm, providing the absorbance at 652 nm. Both systems provided consistent results related to the photosensitizer concentration. The methods show a remarkable difference in the concentration of photosensitizer in normal skin and tumor. No significant difference in mTHPC concentration in tumor could be observed between the 4 and 6h groups after drug application. (Less)
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organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
MTHPC, Skin carcinomas, Fluorescence imaging, Fluorescence spectroscopy
host publication
Optical Diagnostics and Sensing VI
volume
6094
pages
940 - 940
publisher
International Society for Optical Engineering
conference name
Optical Diagnostics and Sensing VI
conference location
San Jose, CA, United States
conference dates
2006-01-24 - 2006-01-25
external identifiers
  • wos:000237703800008
  • scopus:33745298759
ISSN
0277-786X
1996-756X
DOI
10.1117/12.647602
language
English
LU publication?
yes
id
8053a720-5447-4bfb-a9f0-0cc2cf3cc9f4 (old id 617213)
date added to LUP
2007-11-24 10:39:37
date last changed
2019-02-20 04:15:20
@inproceedings{8053a720-5447-4bfb-a9f0-0cc2cf3cc9f4,
  abstract     = {Measurements of concentration of sensitizers for photodynamic therapy can provide important information in the dosimetry planning and can also give input to the optimal time for treatment. There has been skepticism towards fluorescence techniques for this purpose, as the signal depends on the fluorescence yield and optical properties of the tissue. Absorption based techniques, lack on the other hand, often the sensitivity required for many sensitizers with relative weak absorption in a wavelength region where hemoglobin absorption is dominant. A direct comparison between absorption and fluorescence techniques for measuring mTHPC concentration after topical application on hairless SKH-1 mice bearing skin carcinomas has been performed. 20 μl/cm&lt;sup&gt;2&lt;/sup&gt; of m-THPC thermogel (0.5 mg m-THPC/ml) were applied on normal and tumor area and the concentration of mTHPC was measured at 4 and 6 hours after drug application by two methods: 1. A fluorescence imaging system capturing images at two wavelengths (500 and 650 nm) following 405 nm excitation. Signals from different regions of interest were averaged and the intensity ratio at 650 to 500 was calculated. 2. A diffuse reflectance spectroscopy system with a fiber separation of 2 mm, providing the absorbance at 652 nm. Both systems provided consistent results related to the photosensitizer concentration. The methods show a remarkable difference in the concentration of photosensitizer in normal skin and tumor. No significant difference in mTHPC concentration in tumor could be observed between the 4 and 6h groups after drug application.},
  author       = {Johansson, Ann and Svensson, Jenny and Andersson-Engels, Stefan and Bendsöe, Niels and Svanberg, Katarina and Bigio, Irving and Alexandratou, Eleni and Kyriazi, Maria and Yova, Dido and Grafe, Susanna and Trebst, Tilmann},
  issn         = {0277-786X},
  keyword      = {MTHPC,Skin carcinomas,Fluorescence imaging,Fluorescence spectroscopy},
  language     = {eng},
  location     = {San Jose, CA, United States},
  pages        = {940--940},
  publisher    = {International Society for Optical Engineering},
  title        = {mTHPC pharmacokinetics following topical administration},
  url          = {http://dx.doi.org/10.1117/12.647602},
  volume       = {6094},
  year         = {2006},
}