Glutamate oxidase advances the selective bioanalytical detection of the neurotoxic amino acid beta-ODAP in grass pea: A decade of progress
(2004) In Pure and Applied Chemistry 76(4). p.765-775- Abstract
- The search for an enzyme as a reagent for selective bioanalytical detection of the neurotoxic amino acid, beta-N-oxalyl-L-,alpha,beta-diaminopropionic acid, beta-ODAP (found in grass pea, Lathyrus sativus) led to its redox catalytic reaction by glutamate oxidase (GluOx). Homogeneous kinetic studies and an immobilized GluOx reactor-based flow-injection assay were initially made for beta-ODAP with small immobilized GluOx/catalase glutamate-destroying prereactors. The method was applied to examine the toxin content in processed grass pea. The kinetics and the equilibrium of the thermal isomerization of beta-ODAP to the nontoxic isomer alpha-ODAP established that GluOx is specific to the neurotoxin. The first ever GluOx-based amperometric... (More)
- The search for an enzyme as a reagent for selective bioanalytical detection of the neurotoxic amino acid, beta-N-oxalyl-L-,alpha,beta-diaminopropionic acid, beta-ODAP (found in grass pea, Lathyrus sativus) led to its redox catalytic reaction by glutamate oxidase (GluOx). Homogeneous kinetic studies and an immobilized GluOx reactor-based flow-injection assay were initially made for beta-ODAP with small immobilized GluOx/catalase glutamate-destroying prereactors. The method was applied to examine the toxin content in processed grass pea. The kinetics and the equilibrium of the thermal isomerization of beta-ODAP to the nontoxic isomer alpha-ODAP established that GluOx is specific to the neurotoxin. The first ever GluOx-based amperometric biosensor for liquid chromatography (LC) detection was reported in 1997. This biosensor coupled with a refractive index detector improved LC performance. The most recent work with GluOx resulted in MnO2-based screen-printed amperometric biosensor, with offline elimination of glutamate interference by glutamate decarboxylase. A single-shot chemiluminescent sensor developed for hydrogen peroxide is also proposed for beta-ODAP with GluOx application. This decade of progress resulted from studies that included four Ph.D. (Ethiopia, Sweden, Austria), four M.Sc. (Ethiopia, Sweden) and Licentiate (Sweden) theses projects, plus one collaborative project in Sweden. The advances in grass pea research may be regarded as a model north-south cooperation for research and education. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/138494
- author
- Moges, G ; Wodajo, N ; Gorton, Lo LU ; Yigzaw, Yirgalem LU ; Kalcher, K ; Belay, A ; Akalu, G ; Nair, Baboo M LU and Solomon, T
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Pure and Applied Chemistry
- volume
- 76
- issue
- 4
- pages
- 765 - 775
- publisher
- IUPAC
- external identifiers
-
- wos:000222017100006
- scopus:2942672697
- ISSN
- 0033-4545
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Applied Nutrition and Food Chemistry (011001300), Analytical Chemistry (S/LTH) (011001004)
- id
- 815a2427-20d3-4207-b49d-f6e9056a2bc7 (old id 138494)
- alternative location
- http://www.iupac.org/publications/pac/2004/7604/7604x0765.html
- date added to LUP
- 2016-04-01 17:16:03
- date last changed
- 2023-09-19 10:28:23
@article{815a2427-20d3-4207-b49d-f6e9056a2bc7, abstract = {{The search for an enzyme as a reagent for selective bioanalytical detection of the neurotoxic amino acid, beta-N-oxalyl-L-,alpha,beta-diaminopropionic acid, beta-ODAP (found in grass pea, Lathyrus sativus) led to its redox catalytic reaction by glutamate oxidase (GluOx). Homogeneous kinetic studies and an immobilized GluOx reactor-based flow-injection assay were initially made for beta-ODAP with small immobilized GluOx/catalase glutamate-destroying prereactors. The method was applied to examine the toxin content in processed grass pea. The kinetics and the equilibrium of the thermal isomerization of beta-ODAP to the nontoxic isomer alpha-ODAP established that GluOx is specific to the neurotoxin. The first ever GluOx-based amperometric biosensor for liquid chromatography (LC) detection was reported in 1997. This biosensor coupled with a refractive index detector improved LC performance. The most recent work with GluOx resulted in MnO2-based screen-printed amperometric biosensor, with offline elimination of glutamate interference by glutamate decarboxylase. A single-shot chemiluminescent sensor developed for hydrogen peroxide is also proposed for beta-ODAP with GluOx application. This decade of progress resulted from studies that included four Ph.D. (Ethiopia, Sweden, Austria), four M.Sc. (Ethiopia, Sweden) and Licentiate (Sweden) theses projects, plus one collaborative project in Sweden. The advances in grass pea research may be regarded as a model north-south cooperation for research and education.}}, author = {{Moges, G and Wodajo, N and Gorton, Lo and Yigzaw, Yirgalem and Kalcher, K and Belay, A and Akalu, G and Nair, Baboo M and Solomon, T}}, issn = {{0033-4545}}, language = {{eng}}, number = {{4}}, pages = {{765--775}}, publisher = {{IUPAC}}, series = {{Pure and Applied Chemistry}}, title = {{Glutamate oxidase advances the selective bioanalytical detection of the neurotoxic amino acid beta-ODAP in grass pea: A decade of progress}}, url = {{http://www.iupac.org/publications/pac/2004/7604/7604x0765.html}}, volume = {{76}}, year = {{2004}}, }